Time-Domain-Based Methyl Proton NMR with Absolute Quantitation Ability for Targeted Metabolomics

Zou, Denglang; Chen, Tao; He, Liangliang; Wang, Qi; Wang, Qiqi; La, Mencuo; Li, Yulin; Jiang, Ren‐Wang

doi:10.1021/acs.analchem.2c00599

Cited by 2 publications

(1 citation statement)

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“…Recent developments in the fields of metabolomics have benefited from advancements in CryoProbe technology with high-field magnets, which drastically lowered the limit of detection, medicines, and natural products the chance to quantify samples at low concentrations. [17,18]. Besides the precise quantitation ability, NMR also exhibits a unique structure discrimination ability in terms of structure elucidation [19].…”

Section: Introductionmentioning

confidence: 99%

Nuclear magnetic resonance‐based solvent system selection for counter‐current chromatography separation of compounds present in the same high‐performance liquid chromatography peak: Flavonoids in barley seedlings as an example

Chen,

Jia,

Zou

et al. 2023

J of Separation Science

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Partition coefficient is a key parameter for counter-current chromatography separation. High-performance liquid chromatography (HPLC) is the most commonly used tool for the screening of partition coefficients. However, HPLC technology is not applicable to the compounds present in the same chromatographic peak. Nuclear magnetic resonance (NMR) technology could easily distinguish compounds according to their characteristic absorption even if they exist in the same HPLC peak. In this study, two flavonoids present in the same HPLC peak were successfully purified by counter-current chromatography with a solvent system screened by NMR to show the great potential of NMR technology in the screening of the partition coefficient of co-efflux compounds. Through NMR screening, an optimized ethyl acetate/n-buthanol/water (7:3:10, v/v/v) system was applied in this study. As a result, two flavonoids, including 4.8 mg of 3′-methoxyl-6′''-O-feruloylsaponarin and 9.8 mg of 6′''-O-feruloylsaponarin were separated from 15 mg of the mixture. There is only one methoxy group difference between the two flavonoids. This study provides a new strategy for the screening of counter-current chromatography solvent systems and broadens the application scope of counter-current chromatography.

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Section: Introductionmentioning

confidence: 99%