Time-kill profiles and cell-surface morphological effects of crude Polycephalomyces nipponicus Cod-MK1201 mycelial extract against antibiotic-sensitive and -resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

Thammawat, Sutthiwan; Sangdee, Kusavadee; Sangdee, Aphidech

doi:10.4314/tjpr.v16i2.20

Cited by 6 publications

(6 citation statements)

References 21 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low MIC of fungal chloroformic extract (0.05 mg/ml to 0.5 mg/ml) marks its importance and corroborates the earlier reports where MIC of endophytic fungi ranged between 0.156 to 0.625 mg/ml (Mahadevamurthy et al 2016 ). The time kill studies revealed the bactericidal nature of the chloroformic extract, which will be useful for pharmaceutical purposes (Thammawat et al 2017 ). PAE studies are finding importance to work out the dosing schedule of antimicrobial administration in a more scientific way (Devi and Joshi 2014 ) and in the present studies, the chloroformic extract retained its effectiveness for variable times, K .…”

Section: Discussionmentioning

confidence: 99%

Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera

Kaur

Arora

2020

AMB Expr

View full text Add to dashboard Cite

The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial potential and is optimized for physiochemical parameters to enhance the antimicrobial metabolites production. In the classical optimization yeast peptone dextrose medium, inoculum size of two discs, incubation period of 6 days, production temperature of 25 ºC and pH 7 was best supportive for optimal growth and antimicrobial activity whereas maltose and ammonium nitrate were the best carbon and nitrogen sources, respectively. The statistical optimization resulted in up to 1.33 fold increase in antimicrobial activity. Chloroform was found to be the best extractant. The chloroformic extract showed minimum inhibitory concentration ranging from 0.05 to 5 mg/ml and its microbicidal nature was established by viable cell count studies. The efficacy of the extract was also established in terms of post antibiotic effect which ranged from 2 to 20 h. The chloroformic extract exhibited the good antibiofilm potential and was also found to be biosafe. The clinical relevance of the study was justified as it showed good antimicrobial efficacy against some resistant clinical isolates, too.

show abstract

Section: Discussionmentioning

confidence: 99%

Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera

Kaur

Arora

2020

AMB Expr

View full text Add to dashboard Cite

show abstract

“…The bactericidal or bacteriostatic activity of the culture filtrate extract against MRSA DMST 20651 and MSSA DMST 2933 were performed by time -kill assays using a method modified from Thammawat et al [17]. Briefly, bacterial cultures in mid -logarithmic growth phase were standardized to the 0.5 McFarland standard and then they were inoculated into several tubes of MHB containing an equal volume of various concentrations of extract (0.78, 0.39 and 0.195 mg/mL) so as to give final concentrations of 0.39 mg/mL (2x MIC), 0.195 mg/mL (1x MIC), and 0.09 mg/mL (0.5x MIC) extract, respectively.…”

Section: Time-kill Assaymentioning

confidence: 99%

Investigation of antibacterial and anti-cancer activities of Streptomyces sp SRF1 culture filtrate

Sangdee¹,

Buranrat²,

Seephonkai³

et al. 2018

Trop. J. Pharm Res

Self Cite

View full text Add to dashboard Cite

“…SEM and TEM imaging were performed to evaluate the treatment effects on both the bacterial surface and within bacterial cells. The previously described method was used for the preparation of MRSA bacterial samples for scanning electron microscopy (SEM) [36]. Treated bacterial cultures as well as untreated samples that served as controls were incubated in a shaker incubator at 37 • C for 24 h. The resulting samples were centrifuged and prefixed in a 2.5% glutaraldehyde solution at 4 • C for 24 h, after which they were rinsed in PBS and post-fixed with 100% acetone applied at the last stage.…”

Section: Molecular Assay By Sem and Tem Microscopymentioning

confidence: 99%

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

2021

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) infection is detrimental to hospitalized patients. With diminishing choices of antibiotics and the worry about resistance to colistin in synergistic combined therapy, there are suggestions for the use of herbal derivatives. This investigation evaluated the synergistic effects of Nigella sativa (NS) in combination with beta-lactam (β-lactam) antibiotics on extreme drug-resistant (XDR) MRSA isolates. NS concentrations of 10, 7.5, 5.0, 2.5, 1.0, and 0.1 µg/mL, alone and in combination with β-lactam antibiotics, were used to determine the antimicrobial susceptibility of MRSA isolates by the well diffusion method. Time–kill assays were performed using a spectrophotometer, with time–kill curves plotted and synergism ascertained by the fractional inhibitory concentration (FIC). Scanning and transmission electron microscopy were used to gain insight into the mechanism of action of treated groups. Isolates were inhibited by the NS concentrations, with differences in the zones of inhibition being statistically insignificant at p < 0.05. There were statistically significant differences in the time–kill assay for the MRSA isolates. In addition, NS combined with augmentin showed better killing than oxacillin and cefuroxime. The mechanism of action shown by the SEM and TEM results revealed cell wall disruption, which probably created interference that led to bacterial lysis.

show abstract

Time-kill profiles and cell-surface morphological effects of crude Polycephalomyces nipponicus Cod-MK1201 mycelial extract against antibiotic-sensitive and -resistant <i>Staphylococcus aureus</i>

Cited by 6 publications

References 21 publications

Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera

Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera

Investigation of antibacterial and anti-cancer activities of Streptomyces sp SRF1 culture filtrate

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Contact Info

Product

Resources

About