Time-lapse deselection model for human day 3 in vitro fertilization embryos: the combination of qualitative and quantitative measures of embryo growth

Liu, Yanhe; Chapple, Vincent; Feenan, Katie; Roberts, Peter; Matson, Phillip

doi:10.1016/j.fertnstert.2015.11.003

Cited by 107 publications

(77 citation statements)

References 42 publications

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“…Such technology has captured the imagination of practitioners as both a research tool (Costa-Borges et al, 2016;Hardarson et al, 2015;Mölder et al, 2015), comparing embryos resulting from various clinical conditions (Balakier et al, 2016;Gurbuz et al, 2016;Hashimoto et al, 2016;Kaihola et al, 2016;Lindgren et al, 2016), and as a potential prognosticator in clinical practice (Adamson et al, 2016;Chen et al, 2016;Kong et al, 2016;Liu et al, 2016;Milewski et al, 2015;Mizobe et al, 2016;Rubio et al, 2014;VerMilyea et al, 2014;Wu et al, 2016aWu et al, , 2016bYang et al, 2014), although some studies negate this (Freour et al, 2015;Wu et al, 2016aWu et al, , 2016b. Bronet et al (2015) even found distinctive morphokinetic differences between male and female embryos.…”

Section: Discussionmentioning

confidence: 99%

Live births after embryo selection using morphokinetics versus conventional morphology: a retrospective analysis

Fishel¹,

Campbell²,

Montgomery³

et al. 2017

Reproductive BioMedicine Online

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The increasing corpus of clinical studies using time-lapse imaging for embryo selection demonstrates considerable variation in study protocols and only limited-sized study cohorts. Outcome measures are based on implantation or clinical pregnancy; some predict blastulation from early cleavage-stage data, and few have evaluated live birth. Erroneously, most studies treat the embryos as independent variables and do not include patient or treatment variables in the statistical analyses. In this study, cohort size was 14,793 patients and 23,762 cycles. The incidence of live birth (n = 973 deliveries) after embryo selection by objective morphokinetic algorithms was compared with conventional embryology selection parameters (n = 6948 deliveries). A 19% increase in the incidence of live birth was observed when morphokinetic data were used to select embryos for the patient cohort aged younger than 38 years (OR 1.19 with 95% CI 1.06 to 1.34) using their own eggs, and an increase of 37% for oocyte recipients aged over 37 years (OR 1.370; 95% Cl 0.763 to 2.450). This is the largest study of the prospective use of time-lapse imaging algorithms in IVF reporting on live birth outcome, although the nature of purely a closed system versus standard incubation could not be assessed.

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Section: Discussionmentioning

confidence: 99%

Live births after embryo selection using morphokinetics versus conventional morphology: a retrospective analysis

Fishel¹,

Campbell²,

Montgomery³

et al. 2017

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…In addition to these morphokinetic variables other studies (11)(12)(13)(14)(15)(16) focused on qualitative markers of cleavage-stage embryos that were associated with a reduced implantation potential and could be used as deselection criteria such as direct cleavage, multinucleation, and abnormal cleavage patterns. In a recently published study (17) another negative prognostic marker emerged, but in a blastocyst-stage embryo.…”

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confidence: 99%

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Bodri¹,

Sugimoto²,

Serna³

et al. 2016

Fertility and Sterility

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“…Quantitative parameters were the time from pronuclear fading (PNF) to ive-cell stage and duration of three-cell stage. In conclusion this deselecting method reported as a reliable tool for embryo selection [39]. In a retrospective multicentric study, 1664 intracytoplasmic sperm injection (ICSI) cycles were analyzed.…”

Section: Morphokinetic Algorithms For Prediction Of Embryo Implantatimentioning

confidence: 99%

Embryo Morphokinetics Based on Time-Lapse Observation

Tabibnejad¹

2017

Embryo Cleavage

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Embryo incubation and evaluation are critical steps in assisted reproductive technology (ART). Conventionally, embryo assessment has been done by embryologists through removing embryos from a conventional incubator during the culture period. Over recent years, time-lapse systems (TLS) have been established which can take digital images of embryos at key points and time intervals. This technique allows embryologists to assess the embryo quality in the steady culture environment. According to TLS studies and prepared algorithm models, it seems that TLS alone or in combination with conventional morphology can be considered as a useful diagnostic tool to determine high-quality embryos and improve embryonic implantation and pregnancy rates. In addition, there were remarkable diferences between embryo developmental time points and intervals regarding embryo gender, embryo fragmentation, and type of ovarian stimulation protocol. For conident conclusion, time-lapse imaging should be evaluated in further studies, and the system should be evaluated for cost/beneit ratio efectiveness in individual laboratory.Keywords: embryo cleavage, embryo morphokinetics, embryoscope, time-lapse imaging IntroductionAssisted reproductive technology (ART) may help infertile couples to realize their dream to have a child in their family, but pregnancy and live birth rates following in vitro fertilization (IVF) still remain low. It is ideal to identify viable embryos with the highest implantation potential to raise IVF success rates. In the traditional IVF practice, embryo assessments are mainly based on the morphologic observation and grade of embryologists at each stage of oocyte and embryonic development. Some features including oocyte and embryo quality, blastomere numbers and regularity, the percentage of fragmentation, and cytoplasmic granularity have been deined as prognostic indicators of successful pregnancy. This traditional embryo assessment method may have some detrimental efects on embryo growth because frequently opening and closing of incubators often cause to the change of embryo culture environmental steadiness. In order to reduce the inter-and intra-observer diference change, the time-lapse imaging (TLI) has been introduced into in vitro fertilization (IVF) laboratory. The application of time-lapse technology to the clinical IVF laboratory has supported more detailed observations on the embryo development researches quickly.The aim of this chapter is to determine whether TLI is useful for selection of "top quality" embryos for transfer to improve ART outcome rather than conventional morphological evaluation. The possible correlation between embryos' sex, embryo fragmentation, treatment protocols, diferent culture media, and embryo morphokinetics will be examined based on some new researches of TLI facilities. Furthermore, various algorithms and predictive models designed in ART cycles with TLI will be discussed.

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Time-lapse deselection model for human day 3 in vitro fertilization embryos: the combination of qualitative and quantitative measures of embryo growth

Abstract: The proposed model involving both qualitative and quantitative deselection effectively predicts day 3 embryo implantation potential and is applicable to all IVF embryos regardless of insemination method by using PNF as the reference starting time point.

Cited by 107 publications

References 42 publications

Live births after embryo selection using morphokinetics versus conventional morphology: a retrospective analysis

Live births after embryo selection using morphokinetics versus conventional morphology: a retrospective analysis

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Embryo Morphokinetics Based on Time-Lapse Observation

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