Time-lapse systems for embryo incubation and assessment in assisted reproduction

Armstrong, Sarah; Bhide, Priya; Jordan, Vanessa; Pacey, Allan; Marjoribanks, Jane; Farquhar, Cindy

doi:10.1002/14651858.cd011320.pub4

Cited by 98 publications

(52 citation statements)

References 53 publications

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“…Several studies investigated the clinical outcomes of medically assisted reproductive (MAR) cycles, comparing TLT to SIs. 4 A prospective randomized study analyzed blastocyst development and clinical outcomes of 64 single-embryo transfers performed in good prognosis patients to assess if the use of TLT compared to SI incubators can be advantageous in the clinical use. No statistical differences were found in terms of blastocyst quality, implantation, and pregnancy rates between the two groups.…”

Section: Time-lapse Systemmentioning

confidence: 99%

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The clinical use of time-lapse in human-assisted reproduction

Minasi

Greco

Varricchio

et al. 2020

Clin Med�Insights�Reprod�Health

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A major challenge in the assisted reproduction laboratory is to set up reproducible and efficient criteria to identify the embryo with the highest developmental potential. Over the years, several methods have been used worldwide with this purpose. Initially, standard morphology assessment was the only available strategy. It is now universally recognized that besides being a very subjective embryo selection strategy, morphology evaluation alone has a very poor prognostic value. More recently, the availability of time-lapse incubators allowed a continuous monitoring of human embryo development. This technology has spread quickly and many fertility clinics over the world produced a remarkable amount of data. To date, however, a general consensus on which variables, or combination of variables, should play a central role in embryo selection is still lacking. Many confounding factors, concerning both patient features and clinical and biological procedures, have been observed to influence embryo development. In addition, several studies have reported unexpected positive outcomes, even in the presence of abnormal developmental criteria. While it does not seem that time-lapse technology is ready to entirely replace the more invasive preimplantation genetic testing in identifying the embryo with the highest implantation potential, it is certainly true that its application is rapidly growing, becoming progressively more accurate. Studies involving artificial intelligence and deep-learning models as well as combining morphokinetic with other non-invasive markers of embryo development, are currently ongoing, raising hopes for its successful applicability for clinical purpose in the near future. The present review mainly focuses on data published starting from the first decade of 2000, when time-lapse technology was introduced as a routine clinical practice in the infertility centers.

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Section: Time-lapse Systemmentioning

confidence: 99%

“…Despite the ever-growing interest in this field, the use of this technology to improve clinical outcomes remains controversial. 4 – 6 …”

Section: Introductionmentioning

confidence: 99%

The clinical use of time-lapse in human-assisted reproduction

Minasi

Greco

Varricchio

et al. 2020

Clin Med�Insights�Reprod�Health

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“…To improve the efficacy and safety of ART, more precise approaches to embryo quality evaluation are needed, especially because a consensus was reached on single ET in ART cycles that elevated the urgency for accurate assessment of an embryo's implantation potential. Numerous studies reported the application of new tools in research, as well as in clinical practice, such as embryo biopsy and genetic diagnosis, 21 morphokinetic monitoring 22 and omics technology. 23 We believe searching for biomarkers in ECM is a more promising approach from a noninvasive and physiological perspective.…”

Section: Discussionmentioning

confidence: 99%

Comparative secretome profile analysis of cultured immortalized human endometrial stromal cells supplemented with implanted versus nonimplanted blastocyst‐conditioned medium: A preliminary analysis

Xiong

Yao

Chen

et al. 2020

J of Obstet and Gynaecol

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Aim Human endometrial stromal cells (HESCs) were previously shown to be capable of discriminating embryos with different qualities. Here we aimed to compare the specific response of the HESC secretome to implanted blastocyst‐conditioned medium (BCM) versus nonimplanted medium and identify cytokine candidates useful for the assessment of blastocyst implantation. Methods Cleavage embryos were individually cultured in one microdrop of medium for blastocyst formation. The BCM was collected after fresh blastocyst transfer on day 5 and used to supplement HESC culture medium. A high‐throughput antibody array covering 440 cytokines was used to detect the secretory proteins of HESCs supplemented with implanted or nonimplanted BCM. Results A total of 22 differentially expressed proteins were found out of 440 cytokines in the supernatant of HESCs supplemented with BCM from the implanted group compared to the nonimplanted group, including seven upregulated and 15 downregulated proteins. Gene Ontology enrichment analysis showed that the differentially expressed proteins were mainly involved in cell chemotaxis and motility, and ERK1/2 cascade regulation. Kyoto Encyclopedia of Genes and Genomes analysis suggested that the mitogen‐activated protein kinase and phosphatidylinositol 3 kinase/Akt pathways were mainly involved. Conclusion HESCs specifically responded to BCM from different quality blastocysts, a finding that can be used to develop a novel approach for blastocyst quality assessment.

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“…Undoubtedly, the advent of TLM has enabled collection of detailed images dynamically associated with a clinical endpoint. Albeit morphokinetics drive the way forward, further data may be required in supporting their beneficial role 62 leading to the conclusion that static observations may still have a lot to offer. It may be possible that an integration of both observation types, with clearer cut-off points and parameters, will improve clinical pregnancy and live birth rates.…”

Section: Future Research Recommendationsmentioning

confidence: 99%

Evaluating the value of day 0 of an ICSI cycle on indicating laboratory outcome

Maziotis

Sfakianoudis²,

Giannelou

et al. 2020

Sci Rep

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A number of oocyte characteristics have been associated with fertilization, implantation and live-birth rates, albeit without reaching a consensus. This study aims to delineate possible associations between oocyte characteristics, oocyte behavior during intracytoplasmic sperm injection (ICSI), fertilization potential, and laboratory outcomes. Four-hundred and seventy-seven patients, yielding 3452 oocytes, were enrolled in this prospective observational study from 2015 to 2018. Οoplasm granularity was associated with poor embryo quality and higher probabilities of post-ICSI oocytes and embryos discarded in any developmental stage and never selected for embryo transfer or cryopreservation (p < 0.001). Both sudden or difficult ooplasm aspiration, and high or lack of resistance during ICSI were associated with either a poor Zygote-Score or fertilization failure (p < 0.001). Sudden or difficult ooplasm aspiration and high resistance during ICSI penetration were positively associated with resulting to a post-ICSI oocyte or embryo that would be selected for discard. Evaluation of oocyte characteristics and oocyte behavior during ICSI may provide early information regarding laboratory and cycle outcomes. Particularly, ooplasm granularity, and fragmentation of polar body, along with sudden or difficult ooplasm aspiration and high or lack of resistance during ICSI penetration may hinder the outcome of an ICSI cycle. The associations presented herein may contribute towards development of a grading system or a prediction model. Taking into account information on oocytes and ICSI behavior may effectively assist in enhancing IVF outcome rates.

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Time-lapse systems for embryo incubation and assessment in assisted reproduction

Abstract: Analysis 2.1. Comparison 2 TLS utilising embryo selection so ware versus TLS with conventional morphological assessment of still TLS images (trial design 2), Outcome

Cited by 98 publications

References 53 publications

The clinical use of time-lapse in human-assisted reproduction

The clinical use of time-lapse in human-assisted reproduction

Comparative secretome profile analysis of cultured immortalized human endometrial stromal cells supplemented with implanted versus nonimplanted blastocyst‐conditioned medium: A preliminary analysis

Evaluating the value of day 0 of an ICSI cycle on indicating laboratory outcome

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