Time-resolved chemiluminescence strategy for multiplexed immunoassay of clenbuterol and ractopamine

Han, Jing; Gao, Hongfei; Wang, Wenwen; Wang, Zhenxing; Fu, Zhifeng

doi:10.1016/j.bios.2013.03.033

Cited by 34 publications

(13 citation statements)

References 32 publications

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“…A series of immunoassay methods such as ELISA [ 12 ], electrochemical sensors [ 14 ], time-resolved chemiluminescence [ 28 ] and LFIA [ 29 ] have been reported for multi-residue analysis of β-agonists. LFIA method is very convenient and more suitable for field screening.…”

Section: Resultsmentioning

confidence: 99%

“…Most of the reported LFIAs for β-agonist can analyze only one target analyte [ 22 , 25 ]. Some multi-component LFIAs have been developed, which can analyze two or three kinds of β-agonist simultaneously [ 26 , 28 ]. But none of the LFIA based method can provide broad screening for seven kinds of β-agonist with lowest LODs (0.01 ng·mL −1 for CMT).…”

Section: Resultsmentioning

confidence: 99%

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A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

et al. 2018

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An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free β-agonists compete for the binding sites. This enables rapid screening for the 7 β-agonists in a single run that takes about 8 min. Detection limits for the seven β-agonists are <50 pg g−1 of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were ±15%. The assay was applied to the analysis of both using spiked and unspiked pork for β-agonists, and the results compare well to those obtained by HPLC-MS. Graphical abstractSchematic presentation of an ultra sensitive fluorescent nanoparticle based paper based assay for rapid detection of multi β-agonists in pork tissue. Electronic supplementary materialThe online version of this article (10.1007/s00604-018-2730-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

et al. 2018

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“…Moreover, it has better analytical performance, i.e. better analytical sensitivity with lower limit of detection (LOD), than our previously developed competitive direct CL-ELISA formats for CAP [24] and newly reported time-resolved chemiluminescence immunoassay for CLE [17] , where the anti-CAP PAbs and anti-CLE MAb were passively adsorbed to the MTP, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Monitoring the growing number of antibiotics, hormones, endocrine disrupting chemicals, toxins in animal-derived food samples places a high demand on rapid, cheap and reliable screening methods. Recently, increasing attention has been paid to the development of various multiplexed immunoassay (MIA) in a single run to achieve multi-residue determination and serve specific analytical purposes, which has been demonstrated to be promising in food safety, clinical diagnosis and environmental monitoring [14] – [17] . In this study, the goat anti-rabbit and goat anti-mouse immunoglobulins were bound covalently to the 3-aminopropyltriethoxysilane (APTES)-functionalized microtiter plates (MTP) in a leach-proof fashion using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (SNHS) to enhance the sensitivity of the MIA and reduce assay duration [18] – [20] .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Forbidden Chemical Residues in Milk Using Dual-Label Time-Resolved Reverse Competitive Chemiluminescent Immunoassay Based on Amine Group Functionalized Surface

et al. 2014

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In this study, a sensitive dual-label time-resolved reverse competitive chemiluminescent immunoassay was developed for simultaneous detection of chloramphenicol (CAP) and clenbuterol (CLE) in milk. The strategy was performed based on the distinction of the kinetic characteristics of horseradish peroxidase (HRP) and alkaline phosphatase (ALP) in chemiluminesecence (CL) systems and different orders of magnitude in HRP CL value for CAP and ALP CL value for CLE in the chemiluminescent immunoassay. Capture antibodies were covalently bound to the amine group functionalized chemiluminescent microtiter plate (MTP) for efficient binding of detection antibodies for the enzymes labeled CAP (HRP-CAP) and CLE (ALP-CLE). The CL signals were recorded at different time points by the automatic luminometers with significant distinction in the dynamic curves. When we considered the ALP CL value (about 105) of CLE as background for HRP CL signal value (about 107) of CAP, there was no interaction from ALP CL background of CLE and the differentiation of CAP and CLE can be easily achieved. The 50% inhibition concentration (IC50) values of CAP and CLE in milk samples were 0.00501 µg L−1 and 0.0128 µg L−1, with the ranges from 0.0003 µg L−1 to 0.0912 µg L−1 and from 0.00385 µg L−1 to 0.125 µg L−1, respectively. The developed method is more sensitive and of less duration than the commercial ELISA kits, suitable for simultaneous screening of CAP and CLE.

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“…A lot of immunoassay methods such as ELISA (Posyniak et al, 2003), ICA sensors , electrochemical sensors (He et al, 2009) and time-resolved chemiluminescence (Han et al, 2013) have been reported for multi-residue analysis of β-agonizts.…”

Section: Comparison Of Analytical Performance With the Published Methodsmentioning

confidence: 99%

A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues

Wang

2015

Biosensors and Bioelectronics

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Time-resolved chemiluminescence strategy for multiplexed immunoassay of clenbuterol and ractopamine

Cited by 34 publications

References 32 publications

A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

Simultaneous Detection of Forbidden Chemical Residues in Milk Using Dual-Label Time-Resolved Reverse Competitive Chemiluminescent Immunoassay Based on Amine Group Functionalized Surface

A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues

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