Time-Resolved Visible and Infrared Study of the Cyano Complexes of Myoglobin and of Hemoglobin I from Lucina pectinata

Helbing, Jan; Bonacina, Luigi; Pietri, Ruth; Bredenbeck, Jens; Hamm, Peter; Mourik, Frank van; Chaussard, Frédéric; González-González, Alejandro; Chergui, Majed; Ramos-Alvarez, Cacimar; Ruiz, Carlos; López‐Garriga, Juan

doi:10.1529/biophysj.103.036236

Cited by 72 publications

(135 citation statements)

References 46 publications

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“…Excited-state spectra were obtained as described previously [26,27,52]. In short, spectra in the visible spectral range were measured using 400 nm pumping ($100 fs, 1 kHz) and white-light continuum probe beam (340-680 nm) that was generated by focusing a small part of the 800 nm fundamental output of the Ti:S laser into a 1 mm thick CaF 2 window.…”

Section: Time-resolved Uv-vis Absorption Spectra (Ta)mentioning

confidence: 99%

Origin of electronic absorption spectra of MLCT-excited and one-electron reduced 2,2′-bipyridine and 1,10-phenanthroline complexes

Záliš

Consani

Al‐Nahhas

et al. 2011

Inorganica Chimica Acta

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, respectively. UV-Vis spectra of the reduced complexes are dominated by IL transitions, plus weaker MLCT contributions. Excited-state spectra show an intense band in the UV region of $50% IL origin mixed with LMCT (bpy, 373 nm) or MLCT (phen, 307 nm) excitations. Because of the significant IL contribution, this spectral feature is akin to the principal IL band of the anions. In contrast, the excited-state visible spectral pattern arises from predominantly LMCT transitions, any resemblance with the reduced-state visible spectra being coincidental.The Re complexes studied herein are representatives of a broad class of metal a-diimines, for which similar spectroscopic behavior can be expected.

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Section: Time-resolved Uv-vis Absorption Spectra (Ta)mentioning

confidence: 99%

Origin of electronic absorption spectra of MLCT-excited and one-electron reduced 2,2′-bipyridine and 1,10-phenanthroline complexes

Záliš

Consani

Al‐Nahhas

et al. 2011

Inorganica Chimica Acta

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“…By contrast, only a few experiments have been carried out on the rebinding dynamics of ionic molecules to heme proteins. 15 …”

Section: Introductionmentioning

confidence: 99%

Picosecond Dynamics of CN^--Ligated Ferric Cytochrome c after Photoexcitation Using Time-resolved Vibrational Spectroscopy

Kim¹,

Park²,

Chowdhury³

et al. 2010

Bulletin of the Korean Chemical Society

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The dynamics of the CN --ligated ferric cytochrome c (CytcCN) in D2O at 283 K following Q-band photoexcitation at 575 nm was observed using femtosecond time-resolved vibrational spectroscopy. The equilibrium vibrational spectrum of the CN stretching mode of CytcCN shows two overlapping bands: one main band (82%) at 2122 cmwith 23 cm -1 full width at half maximum (fwhm) and the other band (18%) at 2116 cm -1 with 7 cm -1 fwhm. The timeresolved spectra show bleaching of the CN fundamental mode of CytcCN and two absorption features at lower energies. The bleach signal and both absorption features are all formed within the time resolution of the experiment (< 200 fs) and decay with a life time of 1.9 ps. One transient absorption feature, appearing immediately red to the bleach signal, results from the thermal excitation of low-frequency modes of the heme that anharmonically couple to the CN fundamental mode, thereby shifting the CN mode to lower energies. The shift of the CN mode decays with a lifetime of 2 ps, equivalent to the time scale for vibrational cooling of the low-frequency heme modes. The other transient absorption feature, which is 3.3 times weaker than the bleach signal and shifted 27 cm -1 toward lower energies, is attributed to the CN mode in an electronically excited state where the CN bond is weakened with a lowered extinction coefficient. These observations suggest that photoexcited CytcCN mainly undergoes ultrafast radiationless relaxation, causing photo-deligation of CN -from CytcCN highly inefficient. As also observed in CN --ligated myoglobin, inefficient ligand photodissociation might be a general property of CN --ligated ferric hemes.

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“…18. The computation of the amplitude of each exponential decay in function of the wavelength results in the Decay Associated Spectra (DAS).…”

Section: Methodsmentioning

confidence: 99%

A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems

Chauvet

Tibiletti

Caffarri

et al. 2014

Review of Scientific Instruments

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The study of biochemical dynamics by ultrafast spectroscopic methods is often restricted by the limited amount of liquid sample available, while the high repetition rate of light sources can induce photodamage. In order to overcome these limitations, we designed a high flux, sub-ml, capillary flow-cell. While the 0.1 mm thin window of the 0.5 mm cross-section capillary ensures an optimal temporal resolution and a steady beam deviation, the cell-pump generates flows up to ∼0.35 ml/s that are suitable to pump laser repetition rates up to ∼14 kHz, assuming a focal spot-diameter of 100 μm. In addition, a decantation chamber efficiently removes bubbles and allows, via septum, for the addition of chemicals while preserving the closed atmosphere. The minimal useable amount of sample is ∼250 μl.

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Time-Resolved Visible and Infrared Study of the Cyano Complexes of Myoglobin and of Hemoglobin I from Lucina pectinata

Cited by 72 publications

References 46 publications

Origin of electronic absorption spectra of MLCT-excited and one-electron reduced 2,2′-bipyridine and 1,10-phenanthroline complexes

Origin of electronic absorption spectra of MLCT-excited and one-electron reduced 2,2′-bipyridine and 1,10-phenanthroline complexes

Picosecond Dynamics of CN^--Ligated Ferric Cytochrome c after Photoexcitation Using Time-resolved Vibrational Spectroscopy

A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems

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Time-Resolved Visible and Infrared Study of the Cyano Complexes of Myoglobin and of Hemoglobin I from Lucina pectinata

Cited by 72 publications

References 46 publications

Origin of electronic absorption spectra of MLCT-excited and one-electron reduced 2,2′-bipyridine and 1,10-phenanthroline complexes

Origin of electronic absorption spectra of MLCT-excited and one-electron reduced 2,2′-bipyridine and 1,10-phenanthroline complexes

Picosecond Dynamics of CN--Ligated Ferric Cytochrome c after Photoexcitation Using Time-resolved Vibrational Spectroscopy

A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems

Contact Info

Product

Resources

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Picosecond Dynamics of CN^--Ligated Ferric Cytochrome c after Photoexcitation Using Time-resolved Vibrational Spectroscopy