Tip-enhanced ablation and ionization mass spectrometry for nanoscale chemical analysis

Liang, Zhou; Zhang, Shudi; Li, Xiaoping; Wang, Tongtong; Huang, Yan‐Hua; Yang, Zhilin; Tian, Zhong‐Qun

doi:10.1126/sciadv.aaq1059

Cited by 39 publications

(32 citation statements)

References 64 publications

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“…Nanoscale resolution chemical analysis is performed via the formation of 50 to 80 nm craters via laser ablation, and the sputtered material is analyzed. () For decades, researchers have attempted to miniaturize these instruments for security applications, detecting toxic compounds in transportation systems, cancer cells in medicine, environmental field and material science measurements, and travelling to Mars. ()…”

Section: Discussionmentioning

confidence: 99%

Experimental Methods in Chemical Engineering: Mass Spectrometry—MS

2019

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Mass spectrometry identifies the atomic mass of molecules and fragments in the gas phase. The spectrometer ionizes the molecules that then pass through an electric or magnetic field towards a detector. The field modifies the molecule's trajectory and we infer mass from its direction and velocity in a static field or from the stability of its path in a dynamic field. The electric current is amplified and a mass spectrum is generated from the location or timing of the signal from the detector, translated into a plot of the intensity as a function of the mass-over-charge ratio. It is field deployable, measures concentrations in real time with a temporal resolution better than 100 ms, and detection limits of fg. However, the signal drifts with time so we have to calibrate it as frequently as every hour. Calibrating requires multiple mixtures with varying concentrations to map the non-linear response. The Web of Science Core Collection indexed over 60 000 articles that refer to MS (2016 and 2017) with applications ranging from permanent gas analysis, to identifying protein, forensic science, and natural products. The bibliometric software VOSViewer [1] identified four clusters of research related to MS: (1) proteomics, proteins, plasma, and metabolomics; (2) solid phase extraction together with gas chromatography; (3) tandem mass spectrometry and liquid chromatography; and (4) waste water and toxicity. We expect that the technique will continue to evolve with increased sensitivity, lower drift, and greater specificity. Miniaturization efforts should also continue in order to develop faster field deployable instruments.

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Section: Discussionmentioning

confidence: 99%

Experimental Methods in Chemical Engineering: Mass Spectrometry—MS

2019

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“…25 Recent achievements in bio-imaging have led to improved lateral resolutions down to 1 μm. 23,26,27 Small spatial resolution for biological samples is essential to obtain effective information on elemental distribution because the diameter of a single mammalian cell is about or less than 20 μm. However, low lateral resolution of micron scale is not extremely necessary for surface imaging of large bulk materials in centimeter scale to study the uniformity of elemental distribution, such as doped ceramics or crystal materials.…”

Section: Optimization Of La-icp-ms Parametersmentioning

confidence: 99%

Elemental Imaging Of Alumina Ceramic Tube Using Laser Ablation- Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS)

Wang¹

2021

At.Spectrosc.

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Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is an attractive analytical technique for the direct analysis of samples found in the geology, biology, and the environmental and material sciences. However, few reports have discussed the difficulty found in the analysis of curved surface samples by LA-ICP-MS where the main focus is on the curved surface. In this work, LA-ICP-MS was used to map the elemental images of curved surface samples by segmentation and recombination. In addition, the influence of parameters, such as laser spot size, laser fluence, repetition rate, scan speed, dwell time and washout time on lateral resolution were investigated. The developed method was applied to the imaging analysis of lanthanum in an alumina ceramic tube, and the results showed that lanthanum was not uniformly distributed in the tube.

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“…Low‐micrometer‐resolution and high‐throughput studies have been demonstrated with a microscope mode MSI . Near‐field‐based MSI techniques have reportedly achieved a lateral resolution of 350 nm for intact molecules and 50 nm for elements, respectively . Recently, a technique that combines transmission‐mode MALDI and post‐ionization was used to successfully image tissue and cell samples on a scale of approximately one micrometer .…”

Section: Introductionmentioning

confidence: 99%

Micro‐Lensed Fiber Laser Desorption Mass Spectrometry Imaging Reveals Subcellular Distribution of Drugs within Single Cells

et al. 2020

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The visualization of temporal and spatial changes in the intracellular environment has great significance for chemistry and bioscience research. Mass spectrometry imaging (MSI) plays an important role because of its unique advantages, such as being label‐free and high throughput, yet it is a challenge for laser‐based techniques due to limited lateral resolution. Here, we develop a simple, reliable, and economic nanoscale MSI approach by introducing desorption laser with a micro‐lensed fiber. Using this integrated platform, we achieved 300 nm resolution MSI and successfully visualized the distribution of various small‐molecule drugs in subcellular locations. Exhaustive dynamic processes of anticancer drugs, including releasing from nanoparticle carriers entering nucleus of cells, can be readily acquired on an organelle scale. Considering the simplicity and universality of this nanoscale desorption device, it could be easily adapted to most of laser‐based mass spectrometry applications.

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Tip-enhanced ablation and ionization mass spectrometry for nanoscale chemical analysis

Abstract: Plasmon properties of metal tips can be used for ablation, ionization, and mass spectrometry imaging with nanoscale resolution.

Cited by 39 publications

References 64 publications

Experimental Methods in Chemical Engineering: Mass Spectrometry—MS

Experimental Methods in Chemical Engineering: Mass Spectrometry—MS

Elemental Imaging Of Alumina Ceramic Tube Using Laser Ablation- Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS)

Micro‐Lensed Fiber Laser Desorption Mass Spectrometry Imaging Reveals Subcellular Distribution of Drugs within Single Cells

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