Tissue Ablation by a Synergistic Combination of Electroporation and Electrolysis Delivered by a Single Pulse

Phillips, Mary; Krishnan, Hanush; Raju, Narayan; Rubinsky, Boris

doi:10.1007/s10439-016-1624-4

Cited by 30 publications

(33 citation statements)

References 29 publications

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“…Mechanistic understanding of the cell death induced by electrolytic ablation is required to enable its further development for widespread clinical application. Furthermore, understanding cellular changes induced by electrolytic ablation will enhance parameter selection for related electrochemical therapies, including IRE, electrochemotherapy, and gene electrotransfer, which may have synergistic effects when combined with electrolytic ablation 39,40 .…”

Section: Introductionmentioning

confidence: 99%

Electrolytic ablation enables cancer cell targeting through pH modulation

Perkons¹,

Stein²,

Nwaezeapu

et al. 2018

Commun Biol

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Minimally invasive ablation strategies enable locoregional treatment of tumors. One such strategy, electrolytic ablation, functions through the local delivery of direct current without thermal effects, facilitating enhanced precision. However, the clinical application of electrolytic ablation is limited by an incompletely characterized mechanism of action. Here we show that acid and base production at the electrodes precipitates local pH changes causing the rapid cell death that underlies macroscopic tumor necrosis at pH > 10.6 or < 4.8. The extent of cell death can be modulated by altering the local buffering capacity and antioxidant availability. These data demonstrate that electrolytic ablation is distinguished from other ablation strategies via its ability to induce cellular necrosis by directly altering the tumor microenvironment. These findings may enable further development of electrolytic ablation as a curative therapy for primary, early stage tumors.

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Section: Introductionmentioning

confidence: 99%

Electrolytic ablation enables cancer cell targeting through pH modulation

Perkons¹,

Stein²,

Nwaezeapu

et al. 2018

Commun Biol

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“…A preliminary, acute study, in a small rodent animal model, has demonstrated the feasibility of tissue ablation with EDW. The results have shown that a very short, single millisecond EDW can ablate large volumes of tissue without attendant muscle contraction (12). We found that the EDW pulse combines the advantages of electrolytic ablation, reversible electroporation and irreversible electroporation, without the disadvantages.…”

Section: Introduction Introductionmentioning

confidence: 78%

“…For the analyzed lesion (lesion no 6), induced on the diaphragmatic surface of the medial left lobe, this was followed by an exponential decay wave form with the following parameters: peak voltage 960V, capacitance charge -122 pulse time constant 97 ms. The parameters chosen for this study were drawn from the experimental results of (15).…”

Section: Results and Discussion Results And Discussionmentioning

confidence: 99%

Minimally Invasive, Non-Thermal Tissue Ablation with a Single Exponential Decay Electrolytic Electroporation Waveform

Rubinsky¹,

Günther²,

Botea³

et al. 2016

JTMR

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A new minimally invasive tissue ablation technique, that combines the biophysical processes of electroporation and electrolysis, is introduced. The technology employs electrode needles inserted in the target tissue to deliver an Electrolytic Electroporation Waveform (EEW) in the form of an exponential decay voltage, several tens of microsecond long. A case study in a large animal model demonstrates that clinically significant size lesions can be achieved with a single, milliseconds long EEW. Ablation with EEW has major advantages over the comparable tissue ablation techniques of electrolysis, reversible electroporation and irreversible electroporation. EEW is orders of magnitude faster than conventional electrolytic ablation. EEW does not require the use of muscle relaxant as irreversible electroporation and EEW does not require injection of drugs as reversible electroporation. This new technology is simple to use and may become an important addition to the minimally invasive surgery armamentarium.

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“…In several recent papers we have shown that combining electroporation and electrolysis (E2) sagaciously yields a new technology of tissue ablation with certain advantages over tissue ablation by electroporation (reversible or irreversible) or electrolysis alone (Phillips et al, 2015; Phillips et al, 2016; Stehling et al, 2016). We have developed several possible synergistic electroporation and electrolysis (E2) protocols.…”

Section: Introductionmentioning

confidence: 99%

Single exponential decay waveform; a synergistic combination of electroporation and electrolysis (E2) for tissue ablation

Klein

Guenther

Mikus³

et al. 2017

PeerJ

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BackgroundElectrolytic ablation and electroporation based ablation are minimally invasive, non-thermal surgical technologies that employ electrical currents and electric fields to ablate undesirable cells in a volume of tissue. In this study, we explore the attributes of a new tissue ablation technology that simultaneously delivers a synergistic combination of electroporation and electrolysis (E2).MethodA new device that delivers a controlled dose of electroporation field and electrolysis currents in the form of a single exponential decay waveform (EDW) was applied to the pig liver, and the effect of various parameters on the extent of tissue ablation was examined with histology.ResultsHistological analysis shows that E2 delivered as EDW can produce tissue ablation in volumes of clinical significance, using electrical and temporal parameters which, if used in electroporation or electrolysis separately, cannot ablate the tissue.DiscussionThe E2 combination has advantages over the three basic technologies of non-thermal ablation: electrolytic ablation, electrochemical ablation (reversible electroporation with injection of drugs) and irreversible electroporation. E2 ablates clinically relevant volumes of tissue in a shorter period of time than electrolysis and electroporation, without the need to inject drugs as in reversible electroporation or use paralyzing anesthesia as in irreversible electroporation.

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Tissue Ablation by a Synergistic Combination of Electroporation and Electrolysis Delivered by a Single Pulse

Cited by 30 publications

References 29 publications

Electrolytic ablation enables cancer cell targeting through pH modulation

Electrolytic ablation enables cancer cell targeting through pH modulation

Minimally Invasive, Non-Thermal Tissue Ablation with a Single Exponential Decay Electrolytic Electroporation Waveform

Single exponential decay waveform; a synergistic combination of electroporation and electrolysis (E2) for tissue ablation

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