Tissue Adhesive, Self-Healing, Biocompatible, Hemostasis, and Antibacterial Properties of Fungal-Derived Carboxymethyl Chitosan-Polydopamine Hydrogels

Abstract

In this work, fungal mushroom-derived carboxymethyl chitosan-polydopamine hydrogels (FCMCS-PDA) with multifunctionality (tissue adhesive, hemostasis, self-healing, and antibacterial properties) were developed for wound dressing applications. The hydrogel is obtained through dynamic Schiff base cross-linking and hydrogen bonds between FCMCS-PDA and covalently cross-linked polyacrylamide (PAM) networks. The FCMCS-PDA-PAM hydrogels have a good swelling ratio, biodegradable properties, excellent mechanical properties, and a highly interconnected porous structure with PDA microfibrils. Interestingly, the PDA microfibrils were formed along with FCMCS fibers in the hydrogel networks, which has a high impact on the biological performance of hydrogels. The maximum adhesion strength of the hydrogel to porcine skin was achieved at about 29.6 ± 2.9 kPa. The hydrogel had good self-healing and recoverable properties. The PDA-containing hydrogels show good antibacterial properties on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. Moreover, the adhesive hydrogels depicted good viability and attachment of skin fibroblasts and keratinocyte cells. Importantly, FCMCS and PDA combined resulted in fast blood coagulation within 60 s. Hence, the adhesive hydrogel with multifunctionality has excellent potential as a wound dressing material for infected wounds.

Stretchable Semi-Interpenetrating Carboxymethyl Guar Gum-Based Composite Hydrogel for Moisture-Proof Wearable Strain Sensor

“…The peak of CC stretching vibration disappeared. The peak at 1654.5 cm –1 was the CO stretching vibration, and at the same time, the peak CH 2 in-plane shear vibration at 1467.2 cm –1 was observed . This absorption peak was not shown in the spectra of CMGG.…”

“…The peak at 1654.5 cm −1 was the C�O stretching vibration, and at the same time, the peak CH 2 inplane shear vibration at 1467.2 cm −1 was observed. 52 This absorption peak was not shown in the spectra of CMGG. The UV−vis absorption spectrum curve of Au@PDA nanoparticles was exhibited in Figure 1b.…”

“…The main CMGG molecules contain hydrogen bonds that strengthen their effect when combined with the concentration of CMGG, the oxygen-containing group (−COOH) of CMGG, which was cross-linked with borax, and the amino group (−NH 2 ) of PAM. These hydrogen bonds benefit hydrogels’ mechanical properties. ,, In addition, the quantified toughness data is presented in Figure b. The CMGG 1.5 /PAM-Au@PDA 1 hydrogel’s toughness could reach 1.76 MJ m –3 , which was around four times that of the PAM hydrogel (0.44 MJ m –3 ).…”

“…These hydrogen bonds benefit hydrogels' mechanical properties. 23,51,52 In addition, the quantified toughness data is presented in Figure 3b. The CMGG 1.5 /PAM-Au@PDA 1 hydrogel's toughness could reach 1.76 MJ m −3 , which was around four times that of the PAM hydrogel (0.44 MJ m −3 ).…”

Application of chitosan-based materials in surgical or postoperative hemostasis

“…In addition, polydopamine has good cell affinity and adhesion properties and is widely used in biomedicine (Barros et al, 2021). A fungal mushroom-derived CMCS-PDA hydrogel (FCMCS-PDA) was developed in a previous study (Rao et al, 2022). FCMCS also exhibited good antibacterial and procoagulant effects.…”