2016
DOI: 10.24075/brsmu.2016-01-09
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Tissue chemiluminescence as a method of evaluation of superoxide radical producing ability of mitochondria

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Cited by 7 publications
(5 citation statements)
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“…We assessed the antioxidant capacity (AC) of brain tissues by Fe-activated chemiluminescence method on day 7 of experimental stroke modeling [ 38 , 39 ]. Damaged lobes of animals’ brains were excised, rinsed in cold saline, and then homogenized with 0.5 mL PBS per 100 mg of cerebral tissue on ice (0–4 °C).…”
Section: Methodsmentioning
confidence: 99%
“…We assessed the antioxidant capacity (AC) of brain tissues by Fe-activated chemiluminescence method on day 7 of experimental stroke modeling [ 38 , 39 ]. Damaged lobes of animals’ brains were excised, rinsed in cold saline, and then homogenized with 0.5 mL PBS per 100 mg of cerebral tissue on ice (0–4 °C).…”
Section: Methodsmentioning
confidence: 99%
“…There is a large number of various modifications of the chemiluminescent method [ 109 , 110 , 111 , 112 , 113 , 114 ]. The most common modification of the chemiluminescent method for determining total antioxidant capacity is based on the use of luminol (LH 2 ) as an activator of chemiluminescence.…”
Section: Methods To Evaluate Integrated Antioxidant Propertiesmentioning
confidence: 99%
“…Lucigenin is one of the most frequently used reagents to detect the superoxide radical [ 6 , 48 ]. It can also be applied to the study of xanthine and hypoxanthine oxidation by xanthine oxidase [ 49 ] to detect the superoxide radical formed as the result of NADPH oxidase activity [ 49 , 50 , 51 , 52 ] and in ether mitochondria of intact cells [ 53 ] or an isolated mitochondrial suspension [ 54 , 55 ]. Lucigenin-based techniques have recently been developed to detect dopamine [ 56 ] and glutathione [ 57 ].…”
Section: Examples Of Substances That Enhance Chemiluminescencementioning
confidence: 99%