Tissue Collection for Systematic Phenotyping in the Mouse

Antal, Cristina; Teletin, Marius; Wendling, Olivia; Dgheem, Mounzer; Auwerx, Johan; Mark, Manuel

doi:10.1002/0471142727.mb29a04s80

Cited by 10 publications

(10 citation statements)

References 1 publication

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“…Following the sacrifice of the mice, various tissues (large intestine, lung, liver, testis, ovary, brain, spleen, and kidney, small intestine and heart) were collected as previous described (30). The backs of mice were shaved, the skin was wiped with alcohol prior to its removal, then subcutaneous fat was removed and was cut into small pieces convenient for digestion and separation, and the samples (~0.3 cm) were then incubated in dispase II (2.5 U/ml; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at 4°C overnight followed by immersion in DMEM containing 50% (v/v) FBS to inactivate the dispase II.…”

Section: Methodsmentioning

confidence: 99%

Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes

et al. 2018

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Psoriasis is a chronic inflammatory skin disease. Keratinocytes (KCs), as skin-specific cells, serve an important role in the immunopathogenesis of psoriasis. In the present study, transcriptome data derived from psoriasis-like KCs were used together with the reported transcriptome data from the skin/epidermis of patient with psoriasis, excluding known psoriasis-associated genes that have been well described in the previous studies according to GeneCards database, to screen for novel psoriasis-associated genes. According to the human expressed sequence tag of UniGene dataset, six genes that are located near psoriasis-associated loci were highly expressed in skin. Among these six genes, four genes (epiregulin, NIPA like domain containing 4, serpin family B member 7 and WAP four-disulfide core domain 12) were highly expressed in normal mouse epidermis (mainly KCs) and mouse psoriatic epidermis cells, but not in psoriatic dermis cells, which further emphasized the specificity of these genes. Furthermore, in systemic inflammatory response syndrome (SIRS), SERPINB7 showed no difference in expression in immune-activated tissues from SIRS and control mice. It was also found that the mRNA expression levels of SERPINB in lesional skin of patients with psoriasis were significantly higher than in non-lesional psoriatic skin from the same patients. SERPINB7 may be a valuable candidate for further studies. In the present study, a method for identifying novel key pathogenic skin-specific molecules is presented, which may be used for investigating and treating psoriasis.

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Section: Methodsmentioning

confidence: 99%

Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes

et al. 2018

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“…Histology, in Situ Hybridization, Immunohistochemistry, and ␤-Galactosidase Activity Analysis-Tissue collection and systematic histopathology were as described previously (29). Briefly, samples were fixed in 10% (v/v) formalin for 16 h, except for testis, which was fixed in Bouin's fluid for 16 h. All samples were embedded in paraffin, and 5-m-thick sections were stained with either hematoxylin and eosin or periodic acidSchiff.…”

Section: Methodsmentioning

confidence: 99%

The STRA6 Receptor Is Essential for Retinol-binding Protein-induced Insulin Resistance but Not for Maintaining Vitamin A Homeostasis in Tissues Other Than the Eye

Berry

Jacobs

Marwarha³

et al. 2013

Journal of Biological Chemistry

Self Cite

124

148

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“…Tissues were isolated from 10-to 16-wk-old C57Bl/6J male mice. Mouse pancreatic islets and hepatocytes were isolated according to the method described by Luco et al (2008); other tissues, according to the method described by Antal et al (2007). Murine ES cells (CGR8) were maintained as described previously (Skoudy et al 2004).…”

Section: Cell Preparationmentioning

confidence: 99%

Derepression of Polycomb targets during pancreatic organogenesis allows insulin-producing beta-cells to adopt a neural gene activity program

Arensbergen¹,

García-Hurtado²,

Morán³

et al. 2010

Genome Res.

152

158

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The epigenome changes that underlie cellular differentiation in developing organisms are poorly understood. To gain insights into how pancreatic beta-cells are programmed, we profiled key histone methylations and transcripts in embryonic stem cells, multipotent progenitors of the nascent embryonic pancreas, purified beta-cells, and 10 differentiated tissues. We report that despite their endodermal origin, beta-cells show a transcriptional and active chromatin signature that is most similar to ectoderm-derived neural tissues. In contrast, the beta-cell signature of trimethylated H3K27, a mark of Polycomb-mediated repression, clusters with pancreatic progenitors, acinar cells and liver, consistent with the epigenetic transmission of this mark from endoderm progenitors to their differentiated cellular progeny. We also identified two H3K27 methylation events that arise in the beta-cell lineage after the pancreatic progenitor stage. One is a wave of cell-selective de novo H3K27 trimethylation in non-CpG island genes. Another is the loss of bivalent and H3K27me3-repressed chromatin in a core program of neural developmental regulators that enables a convergence of the gene activity state of beta-cells with that of neural cells. These findings reveal a dynamic regulation of Polycomb repression programs that shape the identity of differentiated beta-cells.

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Tissue Collection for Systematic Phenotyping in the Mouse

Cited by 10 publications

References 1 publication

Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes

Systematic screening and identification of novel psoriasis‑specific genes from the transcriptome of psoriasis‑like keratinocytes

The STRA6 Receptor Is Essential for Retinol-binding Protein-induced Insulin Resistance but Not for Maintaining Vitamin A Homeostasis in Tissues Other Than the Eye

Derepression of Polycomb targets during pancreatic organogenesis allows insulin-producing beta-cells to adopt a neural gene activity program

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