Tissue distribution and acute toxicity of silver after single intravenous administration in mice: nano-specific and size-dependent effects

Recordati, Camilla; Maglie, Marcella De; Bianchessi, Silvia; Argentiere, Simona; Cella, Claudia; Mattiello, S.; Cubadda, Francesco; Aureli, Federica; D’Amato, Marilena; Raggi, Andrea; Lenardi, Cristina; Milani, P.; Scanziani, Eugenio

doi:10.1186/s12989-016-0124-x

Cited by 242 publications

(194 citation statements)

References 65 publications

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“…61,62 However, it has been concluded that deposition of AgNPs in tissue samples is sizedependent in the liver and kidneys of male and female rats. 63,64 In this study, Se treatment of AgNPs exposed rats showed alleviation of the histopathological changes in rat livers.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and hepatoprotective role of selenium against silver nanoparticles

Ansar

Alshehri

Abudawood

et al. 2017

IJN

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Silver nanoparticles (AgNPs) have attracted the most interest in terms of their potential biomedical and industrial applications. However, these nanoparticles have shown their toxic behavior toward environment, living tissues, and organisms. Selenium (Se), an essential trace element, is necessary for various metabolic processes, including protection against oxidative stress and immune function. The present study was undertaken to evaluate the effect of Se against AgNP-induced hepatic oxidative stress. AgNPs were synthesized and then prepared nanoparticles were characterized using various analytical techniques such as UV-visible spectroscopy, X-ray diffraction, and transmission electron microscopy. Rats were administered AgNPs intraperitoneally (5 mg/kg/day) and Se (0.2 mg/kg) was given by gavage. AgNP administration induced hepatic damage as indicated by the serum marker enzymes with reduction in levels of glutathione, and decrease in activities of SOD, CAT, and GSH-peroxidase ( P <0.05). Decrease in levels of total antioxidant capacity (TAC) and increase in level of C-reactive protein (CRP) was also observed in AgNP-treated group compared to control group. However, Se markedly attenuated AgNP-induced biochemical alterations, levels of TAC, CRP, and serum transaminases (AST, ALT) ( P <0.05). Taken together, these findings suggest that administration of AgNPs produces hepatotoxicity in rats, whereas Se supplementation attenuates these effects.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and hepatoprotective role of selenium against silver nanoparticles

Ansar

Alshehri

Abudawood

et al. 2017

IJN

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“…In addition, coating has been reported to play an important role in NPs toxicity. In this context, in vivo studies have reported that polyvinylpyrrolidone (PVP) and citrate (CT) coated AgNPs induce tissue specific toxicity and aggravate cardiac ischemic reperfusion injury [20,21]. Moreover, a previous in vitro study has shown that PVPand CT-AgNPs induce toxicity in J774A.1 macrophage and HT29 epithelial cells [22].…”

Section: The In Vitro Effect Of Polyvinylpyrrolidone and Citrate Coatmentioning

confidence: 99%

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Ferdous

Beegam

Tariq

et al. 2018

Cell Physiol Biochem

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Background/Aims: Silver nanoparticles (AgNPs) are increasingly used as antimicrobial agents and drug carriers in various biomedical fields. AgNPs can encounter erythrocytes either directly following intravenous injection, or indirectly via translocation from the site of administration. However, information regarding the pathophysiological effects and possible mechanism of action of AgNPs on the erythrocytes are still inadequately studied. Thus, the aim of our study was to investigate the mechanism underlying the effect of coating and concentration of AgNPs on mouse erythrocytes in vitro. Methods: We studied the interaction of polyvinylpyrrolidone (PVP) and citrate (CT) coated AgNPs (10 nm) at various concentrations (2.5, 10, 40 µg/ml) with mouse erythrocytes in vitro using various techniques including transmission electron microscopy (TEM), hemolysis, and colorimetric measurement of markers of oxidative stress comprising malondialdehyde (MDA), reduced glutathione (GSH), and catalase (CAT). Intracellular calcium (Ca²⁺) was determined using Fura 2AM fluorescence. Annexin V was quantified using ELISA and the caspase 3 was determined both flurometrically and by western blot technique. Results: Following incubation of the erythrocytes with AgNPs, both PVP- and CT- AgNPs induced significant and dose - dependent increase in hemolysis. TEM revealed that both PVP- and CT- AgNPs were taken up by erythrocytes. The erythrocyte susceptibility to lipid peroxidation measured by MDA was significantly increased in both PVP-and CT- AgNPs. The concentration of GSH and CAT activity were significantly decreased by both types of AgNPs. Additionally, PVP- and CT- AgNPs significantly increased intracellular Ca²⁺ in a dose -dependent manner. Likewise, the concentration of the cellular protein annexin V was significantly and dose - dependently enhanced by both types of AgNPs. Furthermore, PVP- and CT- AgNPs induced significant increase in calpain activity in incubated erythrocytes. Conclusion: We conclude that both PVP- and CT- AgNPs causes hemolysis, and are taken up by erythrocytes. Moreover, we demonstrated that AgNPs induces oxidative stress and eryptosis. These findings provide evidence for the potential pathophysiological effect of PVP-and CT- AgNPs on erythrocyte physiology.

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“…The largest share of silver that reaches the blood is filtered by the liver and excreted through the gall. 7,9,15 The scheme of silver treatment has been described depending on the size in the liver and it has been suggested that absorption of silver by the Kupffer cells hardly occurs after administration of 100 nm AgNPs, while the introduction of 10 nm and 40 nm AgNPs led to the absorption of silver both endothelial cells and hepatocytes, in addition to the Kupffer cells. Only occasionally, silver was identified in gallbladder of 10 nm AgNP-treated mice (within the epithelial cells of the gallbladder and endothelial cells of blood vessels).…”

Section: Discussionmentioning

confidence: 99%

“…In lungs silver was found in the alveolar walls, capillaries, and interstitial tissue. 15 In literature, there are data about the impact of nanoparticles on oocytes. [16][17][18] There have been published data about the effect nanoparticles of gold, silver, and gold-silver alloy, which were covered with bovine serum albumin (BSA) on in vitro cumulus-oocytes-cellcomplexes of pigs.…”

Section: Discussionmentioning

confidence: 99%

Functional status of reproductive system under treatment of silver nanoparticles in female mice

Lytvynenko

Rieznichenko

Sribna

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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INTRODUCTIONThe unique desired physico-chemical properties of NPs, such as higher stability in the aquatic environment, decreased size and increased specific surface area and thus enhanced reactivity, which makes nanomaterials more efficient in industrial applications and at the same time make them more harmful for living organisms. 1,2 ABSTRACTBackground: The use of silver nanoparticles (AgNP) has increased very significantly in recent years the work in science is currently on focused on assessing human health and environmental risks of nanotechnology. The aim of the present study was to estimate the functional status of the female reproductive system in mice under condition of the intravenous treatment of silver nanoparticles (AgNPs), namely to assess meiotic maturation of oocytes, viability of follicular cells surrounding the oocyte, spontaneous contractile activity of the myometrium and pre-and postimplantation mortality of embryos. Methods: Research (two series) has been done on white laboratory mice (8 weeks, 16-18 g). AgNPs are spherical nanoparticles of 30 nm (8 mg/ml for metal) diluted in water for injection. Method of treatment: intravenous. Two doses (2 mg/kg and 4 mg/kg) have been investigated. Frequency of treatment: one time per day with each dose of 1, 5 and 10 times (n=10 animals in each group). Material for the study (ovaries, uterus) were taken the day after the last AgNPs injection. Results: Ten-time AgNPs treatment (2 mg/kg and 4 mg/kg) results in inhibition of oocytes meiotic maturation in mice; a single-and five-time AgNPs treatment (2 mg/kg and 4 mg/kg) increases the number of apoptotic cells, while the ten-time AgNPs treatment results in an increase of the apoptotic and necrotic follicular cells surrounding oocytes; for the five-and ten-time AgNPs treatment (4 mg/kg) the index of contractility (IC) of the uterus increased; for the ten-time AgNPs treatment (2 mg/kg and 4 mg/kg) no differences in value of the embryonic mortality between control and study groups have been observed. Conclusions: This study suggests that the development of nanomaterials should be safer and non-toxic and the potential reprotoxicity of AgNPs should be investigated more carefully.

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Tissue distribution and acute toxicity of silver after single intravenous administration in mice: nano-specific and size-dependent effects

Cited by 242 publications

References 65 publications

Antioxidant and hepatoprotective role of selenium against silver nanoparticles

Antioxidant and hepatoprotective role of selenium against silver nanoparticles

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Functional status of reproductive system under treatment of silver nanoparticles in female mice

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