Tissue distribution and metabolic profiling of cyclosporine (CsA) in mouse and rat investigated by DESI and MALDI mass spectrometry imaging (MSI) of whole-body and single organ cryo-sections

Holm, Niels Bjerre; Deryabina, Maria A.; Knudsen, Carsten; Janfelt, Christian

doi:10.1007/s00216-022-04269-z

Cited by 18 publications

(10 citation statements)

References 30 publications

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“…(MALDI) was also recently used to study the distribution of therapeutic peptides in a murine model. 120 The study imaged the immunosuppressant cyclosporine and its metabolites throughout the internal organs, demonstrating the drug itself was primarily detected in the pancreas and liver, whereas metabolites were primarily found in the intestines.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

“…Despite challenges with low signal intensity and severe ion suppression, the authors were able to demonstrate the localization of drug metabolites in specific regions of the kidney and widespread distribution throughout liver tissue. DESI and matrix‐assisted laser desorption ionization (MALDI) was also recently used to study the distribution of therapeutic peptides in a murine model 120 . The study imaged the immunosuppressant cyclosporine and its metabolites throughout the internal organs, demonstrating the drug itself was primarily detected in the pancreas and liver, whereas metabolites were primarily found in the intestines.…”

Section: Applications Of Aimsmentioning

confidence: 99%

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Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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Section: Pharmaceuticalsmentioning

confidence: 99%

Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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“…The tissue sections are then mounted onto the glass slides, metal targets or indium-tin oxide (ITO) coated glass slides. Before sectioning fresh frozen tissues, various embedding media can be used to preserve the morphology of the tissues and assist with the tissue section, including optimal cutting temperature medium (OCT), carboxymethyl cellulose, gelatin, agarose or ice (121,(187)(188)(189)(190)(191). However, OCT could suppress analyte signals and is not recommended in MSI (189,192).…”

Section: Sample Processingmentioning

confidence: 99%

Applications of spatially resolved omics in the field of endocrine tumors

et al. 2023

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Endocrine tumors derive from endocrine cells with high heterogeneity in function, structure and embryology, and are characteristic of a marked diversity and tissue heterogeneity. There are still challenges in analyzing the molecular alternations within the heterogeneous microenvironment for endocrine tumors. Recently, several proteomic, lipidomic and metabolomic platforms have been applied to the analysis of endocrine tumors to explore the cellular and molecular mechanisms of tumor genesis, progression and metastasis. In this review, we provide a comprehensive overview of spatially resolved proteomics, lipidomics and metabolomics guided by mass spectrometry imaging and spatially resolved microproteomics directed by microextraction and tandem mass spectrometry. In this regard, we will discuss different mass spectrometry imaging techniques, including secondary ion mass spectrometry, matrix-assisted laser desorption/ionization and desorption electrospray ionization. Additionally, we will highlight microextraction approaches such as laser capture microdissection and liquid microjunction extraction. With these methods, proteins can be extracted precisely from specific regions of the endocrine tumor. Finally, we compare applications of proteomic, lipidomic and metabolomic platforms in the field of endocrine tumors and outline their potentials in elucidating cellular and molecular processes involved in endocrine tumors.

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“…When coupled to a mass spectrometer (MS), DESI-MS allows atmospheric detection of ions from tissue surfaces. 15 DESI-MSI has been routinely used to detect and spatially map lipids 16 and small molecules 17 after its introduction in 2004. 18 Additionally, it has been used frequently to accurately detect drug targets with good spatial resolution.…”

Section: ■ Introductionmentioning

confidence: 99%

Improved Detection of Tryptic Peptides from Tissue Sections Using Desorption Electrospray Ionization Mass Spectrometry Imaging

Bottomley,

Phillips,

Hart

2024

J. Am. Soc. Mass Spectrom.

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DESI-MSI is an ambient ionization technique used frequently for the detection of lipids, small molecules, and drug targets. Until recently, DESI had only limited use for the detection of proteins and peptides due to the setup and needs around deconvolution of data resulting in a small number of species being detected at lower spatial resolution. There are known differences in the ion species detected using DESI and MALDI for nonpeptide molecules, and here, we identify that this extends to proteomic species. DESI MS images were obtained for tissue sections of mouse and rat brain using a precommercial heated inlet (approximately 450 °C) to the mass spectrometer. Ion mobility separation resolved spectral overlap of peptide ions and significantly improved the detection of multiply charged species. The images acquired were of pixel size 100 μm (rat brain) and 50 μm (mouse brain), respectively. Observed tryptic peptides were filtered against proteomic target lists, generated by LC−MS, enabling tentative protein assignment for each peptide ion image. Precise localizations of peptide ions identified by DESI and MALDI were found to be comparable. Some spatially localized peptides ions were observed in DESI that were not found in the MALDI replicates, typically, multiply charged species with a low mass to charge ratio. This method demonstrates the potential of DESI-MSI to detect large numbers of tryptic peptides from tissue sections with enhanced spatial resolution when compared to previous DESI-MSI studies.

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Tissue distribution and metabolic profiling of cyclosporine (CsA) in mouse and rat investigated by DESI and MALDI mass spectrometry imaging (MSI) of whole-body and single organ cryo-sections

Cited by 18 publications

References 30 publications

Applications of ambient ionization mass spectrometry in 2022: An annual review

Applications of ambient ionization mass spectrometry in 2022: An annual review

Applications of spatially resolved omics in the field of endocrine tumors

Improved Detection of Tryptic Peptides from Tissue Sections Using Desorption Electrospray Ionization Mass Spectrometry Imaging

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