Tissue engineered ultra-thin descemet stripping corneal endothelial layers using porcine cornea and stem cells

An, Jeong-Hee; Park, Soyoung; Kim, Gonhyung; Mo, In-Pil; Kim, Soochong; Woo, Heung‐Myong; Park, Kyung-Mee

doi:10.1016/j.exer.2020.108192

Cited by 6 publications

(10 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, they found that longer decellularization time led to lower transparency of grafts. 58 This finding was consistent with another study which found that using 0.5% or 1% SDS for 24 h led to an opaque and swollen decellularized porcine cornea matrix. 59 Another study found that using 2% Triton X-100 for decellularization of human corneal stroma produced a scaffold with acceptable mechanical properties and integral ECM proteins, which supported the biological functions of cells.…”

Section: Decellularized Tissuessupporting

confidence: 92%

Section: Biomaterialssupporting

confidence: 92%

“…Fortunately, transparency results of scaffolds were in fact outstanding in some biomaterials such as silk fibroin, 71 , 72 pεK, 118 , 120 chitosan, 107 , 112 agarose, 114 decellularized human crystalline LCs, 63 and decellularized DS from porcine cornea. 58 …”

Section: Discussionmentioning

confidence: 99%

“…The scaffold was shown to be a homogeneously reseeded graft after recellularization of cells on it, and the functional markers expression of CECs were confirmed by immunohistochemistry. 58 …”

Section: Biomaterialsmentioning

confidence: 99%

“…At the same time, this product must not adversely influence the tridimensional (3D) structure, organization, and mechanical integrity of the native ECM tissue. 56,57 As a matter of fact, sodium dodecyl sulfate (SDS) 58,59 and Triton X-100 27 are widely utilized in the decellularization process of the cornea.…”

Section: Decellularized Tissuesmentioning

confidence: 99%

See 4 more Smart Citations

Use of biomaterials in corneal endothelial repair

2021

View full text Add to dashboard Cite

Human corneal endothelium (HCE) is a single layer of hexagonal cells that lines the posterior surface of the cornea. It forms the barrier that separates the aqueous humor from the rest of the corneal layers (stroma and epithelium layer). This layer plays a fundamental role in maintaining the hydration and transparency of the cornea, which in turn ensures a clear vision. In vivo, human corneal endothelial cells (HCECs) are generally believed to be nonproliferating. In many cases, due to their nonproliferative nature, any damage to these cells can lead to further issues with Descemet’s membrane (DM), stroma and epithelium which may ultimately lead to hazy vision and blindness. Endothelial keratoplasties such as Descemet’s stripping automated endothelial keratoplasty (DSAEK) and Descemet’s membrane endothelial keratoplasty (DEK) are the standard surgeries routinely used to restore vision following endothelial failure. Basically, these two similar surgical techniques involve the replacement of the diseased endothelial layer in the center of the cornea by a healthy layer taken from a donor cornea. Globally, eye banks are facing an increased demand to provide corneas that have suitable features for transplantation. Consequently, it can be stated that there is a significant shortage of corneal grafting tissue; for every 70 corneas required, only 1 is available. Nowadays, eye banks face long waiting lists due to shortage of donors, seriously aggravated when compared with previous years, due to the global COVID-19 pandemic. Thus, there is an urgent need to find alternative and more sustainable sources for treating endothelial diseases, such as utilizing bioengineering to use of biomaterials as a remedy. The current review focuses on the use of biomaterials to repair the corneal endothelium. A range of biomaterials have been considered based on their promising results and outstanding features, including previous studies and their key findings in the context of each biomaterial.

show abstract

Section: Decellularized Tissuessupporting

confidence: 92%

Section: Biomaterialssupporting

confidence: 92%