Tissue engineering: chondrocyte cultures on type I collagen support. Cytohistological and immunohistochemical study

Negri, Stefano; Farinato, S; Bellomi, A; Fila, Chiara; Pagliaro, Pasquale

doi:10.1007/s10195-007-0169-6

Cited by 5 publications

(6 citation statements)

References 38 publications

(89 reference statements)

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“…Morphologically, this tissue is characterized by the presence of a small number of cells, called chondrocytes, which are responsible for the production, organization and renewal of extracellular matrix (ECM). The latter corresponds to most of the tissue’s dry weight, surrounding all chondrocytes and maintaining a strong structural and functional relationship with these cells [2,3]. …”

Section: Introductionmentioning

confidence: 99%

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Ciccone

Zuzzi²,

Neves³

et al. 2013

BMC Complement Altern Med

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BackgroundIn this study, we investigate the effects of microcurrent stimulation on the repair process of xiphoid cartilage in 45-days-old rats.MethodsTwenty male rats were divided into a control group and a treated group. A 3-mm defect was then created with a punch in anesthetized animals. In the treated group, animals were submitted to daily applications of a biphasic square pulse microgalvanic continuous electrical current during 5 min. In each application, it was used a frequency of 0.3 Hz and intensity of 20 μA. The animals were sacrificed at 7, 21 and 35 days after injury for structural analysis.ResultsBasophilia increased gradually in control animals during the experimental period. In treated animals, newly formed cartilage was observed on days 21 and 35. No statistically significant differences in birefringent collagen fibers were seen between groups at any of the time points. Treated animals presented a statistically larger number of chondroblasts. Calcification points were observed in treated animals on day 35. Ultrastructural analysis revealed differences in cell and matrix characteristics between the two groups. Chondrocyte-like cells were seen in control animals only after 35 days, whereas they were present in treated animals as early as by day 21. The number of cuprolinic blue-stained proteoglycans was statistically higher in treated animals on days 21 and 35.ConclusionWe conclude that microcurrent stimulation accelerates the cartilage repair in non-articular site from prepuberal animals.

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Section: Introductionmentioning

confidence: 99%

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Ciccone

Zuzzi²,

Neves³

et al. 2013

BMC Complement Altern Med

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“…AC, which is located in the diarthrodial joint, is a high mechanical stress–bearing tissue and therefore, structurally it represents the uniqueness of HC organization. 8 , 9 , 24 , 26 It is composed of 4 different layers ( Figure 1 ) that differ in their protein content, proteoglycan composition, thickness of the collagen network, and cellular content. 3 , 9 , 16 , 26 , 30 Among all the layers, the deep layer (layer III [LIII]) of the native AC is the most stress resistant because it comprises thick collagen fibrils, high PG content, and decreased water concentration.…”

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confidence: 99%

Reconstruction of Hyaline Cartilage Deep Layer Properties in 3-Dimensional Cultures of Human Articular Chondrocytes

Nanduri

Tattikota

Raj

et al. 2014

Orthopaedic Journal of Sports Medicine

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Background:Articular cartilage (AC) injuries and malformations are commonly noticed because of trauma or age-related degeneration. Many methods have been adopted for replacing or repairing the damaged tissue. Currently available AC repair methods, in several cases, fail to yield good-quality long-lasting results, perhaps because the reconstructed tissue lacks the cellular and matrix properties seen in hyaline cartilage (HC).Purpose:To reconstruct HC tissue from 2-dimensional (2D) and 3-dimensional (3D) cultures of AC-derived human chondrocytes that would specifically exhibit the cellular and biochemical properties of the deep layer of HC.Study Design:Descriptive laboratory study.Methods:Two-dimensional cultures of human AC–derived chondrocytes were established in classical medium (CM) and newly defined medium (NDM) and maintained for a period of 6 weeks. These cells were suspended in 2 mm–thick collagen I gels, placed in 24-well culture inserts, and further cultured up to 30 days. Properties of chondrocytes, grown in 2D cultures and the reconstructed 3D cartilage tissue, were studied by optical and scanning electron microscopic techniques, immunohistochemistry, and cartilage-specific gene expression profiling by reverse transcription polymerase chain reaction and were compared with those of the deep layer of native human AC.Results:Two-dimensional chondrocyte cultures grown in NDM, in comparison with those grown in CM, showed more chondrocyte-specific gene activity and matrix properties. The NDM-grown chondrocytes in 3D cultures also showed better reproduction of deep layer properties of HC, as confirmed by microscopic and gene expression analysis. The method used in this study can yield cartilage tissue up to approximately 1.6 cm in diameter and 2 mm in thickness that satisfies the very low cell density and matrix composition properties present in the deep layer of normal HC.Conclusion:This study presents a novel and reproducible method for long-term culture of AC-derived chondrocytes and reconstruction of cartilage tissue with properties similar to the deep layer of HC in vitro.Clinical Relevance:The HC tissue obtained by the method described can be used to develop an implantable product for the replacement of damaged or malformed AC, especially in younger patients where the lesions are caused by trauma or mechanical stress.

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“…Less and still experimental are healing attempts of human soft tissues such as tendon [ 58 , 177 , 178 , 179 ], peripheral nerve [ 105 , 115 ] and adipose tissue [ 161 ]. Notwithstanding, clinically approved are equine tendon collagen devices for dura mater [ 111 , 112 , 113 , 114 ] and cartilage [ 123 , 124 , 131 , 132 , 133 , 134 , 141 , 180 , 181 , 182 , 183 , 184 ] repair.…”

Section: Currently Approved Equine Collagen-based Devicesmentioning

confidence: 99%

“…Current strategies for the regeneration of cartilage usually involve the use of hyaluronic acid-based formulations for its viscosupplementation properties and collagen (MeRG ® , MaioRegen ® ) for its pro-regenerative properties [ 182 , 185 ]. The needing of a regenerative support is due to the fact that the intrinsic healing potential of the damaged cartilage is limited because of the absence of blood vessels and innervation [ 180 ]. The missing of vascularization stimulation properties is the reason why most of the products on the market fail to provide long-term results [ 183 ].…”

Section: Currently Approved Equine Collagen-based Devicesmentioning

confidence: 99%

“…The missing of vascularization stimulation properties is the reason why most of the products on the market fail to provide long-term results [ 183 ]. Nevertheless, equine tendon collagen scaffolds in vitro revealed their potential in cartilage regeneration by promoting and supporting chondrocytes growth and proliferation [ 141 , 180 , 181 , 183 , 184 ]. Various cell populations seeded and cultured on pure type I collagen, multiplicate and express chondrocyte cells phenotype (i.e., expression of vimentin, Sox9, Matrilin-1, S100, CD99, aggrecan, collagen type II), accompanied by the production of ECM (i.e., immunohistochemical analysis on collagen type I, type II and proteoglycans) [ 180 , 181 , 183 ].…”

Section: Currently Approved Equine Collagen-based Devicesmentioning

confidence: 99%

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An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications

Gallo

Natali

Sannino

et al. 2020

JFB

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Type I collagen has always aroused great interest in the field of life-science and bioengineering, thanks to its favorable structural properties and bioactivity. For this reason, in the last five decades it has been widely studied and employed as biomaterial for the manufacture of implantable medical devices. Commonly used sources of collagen are represented by bovine and swine but their applications are limited because of the zoonosis transmission risks, the immune response and the religious constrains. Thus, type-I collagen isolated from horse tendon has recently gained increasing interest as an attractive alternative, so that, although bovine and porcine derived collagens still remain the most common ones, more and more companies started to bring to market a various range of equine collagen-based products. In this context, this work aims to overview the properties of equine collagen making it particularly appealing in medicine, cosmetics and pharmaceuticals, as well as its main biomedical applications and the currently approved equine collagen-based medical devices, focusing on experimental studies and clinical trials of the last 15 years. To the best of our knowledge, this is the first review focusing on the use of equine collagen, as well as on equine collagen-based marketed products for healthcare.

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Tissue engineering: chondrocyte cultures on type I collagen support. Cytohistological and immunohistochemical study

Cited by 5 publications

References 38 publications

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Reconstruction of Hyaline Cartilage Deep Layer Properties in 3-Dimensional Cultures of Human Articular Chondrocytes

An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications

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