Tissue Engineering for Cutaneous Wounds

Clark, Richard A.F.; Ghosh, Kaustabh; Tonnesen, Marcia G.

doi:10.1038/sj.jid.5700715

Cited by 468 publications

(426 citation statements)

References 106 publications

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“…4), similar to when the dermis of normal skin is wounded [9,12]. When the construct is implanted into the body the fibroblasts should eventually replace the degrading alginate with natural ECM.…”

Section: Resultsmentioning

confidence: 99%

“…The first tissue-engineered skin analogue was developed in 1975, which simply aimed to replace the epidermal layer using stratified keratinocyte cultures [6]. Since then, a number of other substitutes have been developed which aim to replace either the dermal layer alone or both the epidermal and dermal layers [7][8][9]. However, the problem with many of these is the presence of human-or animal-derived components such as de-epidermalised dermis (DED) or collagen, which carry the risk of disease transmission and promoting immune rejection [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…However, the problem with many of these is the presence of human-or animal-derived components such as de-epidermalised dermis (DED) or collagen, which carry the risk of disease transmission and promoting immune rejection [10,11]. It has also been suggested that the inclusion of collagen in the initial construct may be detrimental to the healing process in terms of tissue cell migration as collagen appears at the later stages of wound healing [9,12]. There is, therefore, a significant need of development of a bilayered structure capable of assisting in wound healing in which the dermal layer is derived from a non-animal source [5,11].…”

Section: Introductionmentioning

confidence: 99%

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An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co‐culture

Hunt

Shelton

Grover

2009

Biotechnology Journal

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International audienceThere is significant interest in the development of tissue engineered skin analogues which replace both the dermal and the epidermal layer, without the use of animal or human derived products such as collagen or de-epidermalised dermis (DED). In this study, we proposed that alginate hydrogel could be used to encapsulate fibroblasts and that keratinocytes could be cultured on the surface to form a bilayered structure which could be used to deliver the co-culture to a wound bed, initially providing wound closure and eventually expediting the healing process. Encapsulation of fibroblasts in 5% alginate hydrogel effectively inhibited their proliferation, whilst maintaining cell viability allowing keratinocytes to grow uninhibited by fibroblast overgrowth to produce a stratified epidermal layer. It was shown that the alginate degradation process was not influenced by the presence of fibroblasts within the hydrogel and that lowering the alginate concentration to 2% w/v increased the rate of degradation. Cells released from the scaffold were able to secrete extra-cellular matrix (ECM) and thus should replace the degrading scaffold with normal ECM following application to the wound site. These findings demonstrate that alginate hydrogel may be an effective delivery vehicle and scaffold for the healing of full-thickness skin wounds

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co‐culture

Hunt

Shelton

Grover

2009

Biotechnology Journal

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“…[1] The loss of skin integrity often leads to disability or even death. [2] However, cutaneous wound healing is one of the most complex biological processes characterized by hierarchical and delicate coordination of multiple cells, molecular regulators, pathways as well as scaffold synthesis. [3][4][5] Full-thickness skin wounds commonly lead to nonfunctioning scarring in adults.…”

Section: Introductionmentioning

confidence: 99%

Pro‐Regenerative Hydrogel Restores Scarless Skin during Cutaneous Wound Healing

Sun

2017

Adv Healthcare Materials

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The transformation of fibrotic healing process to regenerative one has great potential to fully restore wounded skin. The M2 macrophage phenotype promotes constructive tissue remodeling and instructs tissue repair in a regenerative manner. It is hypothesized that hydrogels that can establish robustness of endogenous cells to regulate M2 phenotype will promote constructive dermal remodeling. Toward this end, a series of dextran‐based bioabsorbable hydrogels are developed and self‐crosslinkable dextran‐isocyanatoethyl methacrylate‐ethylamine (DexIEME) is identified as the potential scaffold. The initial screening study revealed that DexIEME has superior biocompatibility in varying concentrations. Although DexIEME brings about low proinflammatory responses, it promotes M2 macrophage phenotype. Then the optimized hydrogel formulation is tested for acute skin injuries using both murine and porcine models. Preliminary data demonstrated that the innovative DexIEME hydrogel promotes complete skin regeneration with hair regrowth on pre‐existing scars, while untreated scars remain intact. Preclinical studies further demonstrated that the DexIEME hydrogel regenerated perfect skin during deep porcine wound healing. Overall, the approach to investigate immune‐modulated hydrogels yields pro‐regenerative DexIEME hydrogel, which may lead to greater clinical success in treating deep dermal injury and attenuating scar formation.

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“…[1][2][3] However, to main tain its struc tu re and func tion, ade qua te levels of nutrients such as pro teins, car bohy dra tes, vita mins and mine rals are requi red. [4][5][6] Malnutrition may be des cri bed as a defi cit of energy, pro tein and/or any other spe ci fic nutrient that leads to a mea su ra ble alte ra tion in body func tion and that is asso cia ted with increa sing the seve rity of a disea se.…”

Section: Introductionmentioning

confidence: 99%

Modelos experimentais de desnutrição e sua influência no trofismo cutâneo

Leite

Júnior

Andrade

et al. 2011

An. Bras. Dermatol.

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BACKGROUND: The skin requires adequate levels of nutrients to function properly. Objective: To analyze skin trophism in well-nourished and undernourished rats using two models of malnutrition. METHODS: In the marasmus model, 60 Wistar rats were kept on a controlled diet, 30 being randomly selected to receive half the established diet for 60 days. In the gelatin model, 60 rats were used, 30 of which received a diet consisting of poor quality protein (gelatin) for 30 days. The nutritional status of the animals was evaluated according to body mass index, clinical signs and serum albumin measurement. After the period of malnutrition, histology was performed on the animals' skin to analyze the thickness of the dermis and epidermis using the Leica Application Suite software. Collagen was analyzed on slides stained with Gömöri trichrome using the ImageJ software program. RESULTS: The body mass index of the malnourished animals in the marasmus and gelatin groups was significantly lower than that of the well-nourished animals in the two groups (p<0.0001 in both models). With respect to serum albumin, there was no difference between the groups in either of the two models. In relation to the histological analysis of skin thickness, the dermis of the malnourished animals was significantly thinner compared to that of the well-nourished animals (p<0.0001 in both models). The percentage of collagen was lower in the malnourished animals compared to the well-nourished animals (p<0.0005 and p<0.003 in the marasmus and gelatin model, respectively). CONCLUSIONS: Skin thickness measurements were lower in the malnourished animals in both models, and this finding was histologically confirmed by the lower percentage of collagen, showing the negative effect of malnutrition on skin trophism. Keywords: Collagen; gelatin; image processing, computer-assisted; nutritional marasmus; public health; skin.Resumo: FUNDAMENTOS: A pele, para exercer suas funções, necessita de níveis adequados de nutrientes. OBJETIVO: Analisar o trofismo cutâneo de ratos nutridos e desnutridos por meio de dois modelos de desnutrição. MÉTODOS: No Modelo Marasmo, utilizaram-se 60 ratos Wistar em controle dietético, dos quais 30 foram selecionados aleatoriamente para receber metade da dieta diária durante 60 dias. No Modelo Gelatina, empregaram-se 60 ratos, dos quais 30 receberam dieta associada a proteína de baixa qualidade (gelatina) durante 30 dias. Avaliou-se o estado nutricional dos animais por meio da massa corporal, dos sinais clínicos e da dosagem de albumina sérica. Após o período de desnutrição, fez-se a histologia da pele dos animais para análise da espessura da derme e epiderme com o software Leica Application Suite; nas lâminas coradas com tricrômio de Gomori, analisou-se a colagênese com o software ImageJ. RESULTADOS: A massa corporal dos animais desnutridos pelo marasmo e gelatina foi significativamente menor (p<0,0001 e p<0,0001) do que a dos grupos nutridos. Quanto à albumina sérica, não houve diferença entre os grupos nos dois modelos. Em re...

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Tissue Engineering for Cutaneous Wounds

Cited by 468 publications

References 106 publications

An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co‐culture

An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co‐culture

Pro‐Regenerative Hydrogel Restores Scarless Skin during Cutaneous Wound Healing

Modelos experimentais de desnutrição e sua influência no trofismo cutâneo

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