Tissue engineering toward organ-specific regeneration and disease modeling

Mandrycky, Christian; Phong, Kiet; Zheng, Ying

doi:10.1557/mrc.2017.58

Cited by 65 publications

(40 citation statements)

References 159 publications

(171 reference statements)

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“…The functional regeneration of damaged or diseased tissues and organs remains an intriguing challenge for modern medicine [1]. Advances in the research field on regenerative biomaterials have inspired successful strategies for the development of smart, affordable and safe scaffolds which and iodate (IO3 − ) ions before and after reaction with polyvinyl alcohol (PVA).…”

Section: Introductionmentioning

confidence: 99%

Halogen-Mediated Partial Oxidation of Polyvinyl Alcohol for Tissue Engineering Purposes

Barbon

Stocco

Dalzoppo

et al. 2020

IJMS

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Partial oxidation of polyvinyl alcohol (PVA) with potassium permanganate turned out to be an efficient method to fabricate smart scaffolds for tissue engineering, endowed with biodegradation and protein delivery capacity. This work considered for the first time the use of halogens (bromine, chlorine and iodine) as less aggressive agents than potassium permanganate to perform controlled PVA oxidation, in order to prevent degradation of polymer molecular size upon chemical modification. Oxidized PVA solutions were chemically characterized (i.e., dinitrophenylhydrazine assay, viscosity measurements, molecular size distribution) before preparing physically cross-linked hydrogels. Scaffolds were assessed for their mechanical properties and cell/tissue biocompatibiliy through cytotoxic extract test on IMR-90 fibroblasts and subcutaneous implantation into BALB/c mice. According to chemical investigations, bromine and iodine allowed for minor alteration of polymer molecular weight. Uniaxial tensile tests demonstrated that oxidized scaffolds had decreased mechanical resistance to deformation, suggesting tunable hydrogel stiffness. Finally, oxidized hydrogels exhibited high biocompatibility both in vitro and in vivo, resulting neither to be cytotoxic nor to elicit severe immunitary host reaction in comparison with atoxic PVA. In conclusion, PVA hydrogels oxidized by halogens were successfully fabricated in the effort of adapting polymer characteristics to specific tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Halogen-Mediated Partial Oxidation of Polyvinyl Alcohol for Tissue Engineering Purposes

Barbon

Stocco

Dalzoppo

et al. 2020

IJMS

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“…However, the current techniques still show important limitations that impede the obtainment of a complete and functional whole-organ. In particular, while a piecemeal of renal components were reconstructed in vitro, the nephron structure has not yet been recreated [ 198 ].…”

Section: Tissue and Organ Regeneration Approachesmentioning

confidence: 99%

“…Nevertheless, they led to the production of functionally immature contractile cardiac constructs [ 202 , 203 , 204 ]. Furthermore, although cardiomyocytes represent the key cell type of this tissue, the engrafting of stromal and endothelial populations, for the recreation of vascular system, remains the most immediate need for obtaining functional organs [ 198 ].…”

Section: Tissue and Organ Regeneration Approachesmentioning

confidence: 99%

Current Advances in 3D Tissue and Organ Reconstruction

Pennarossa

Arcuri

Iorio

et al. 2021

IJMS

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Bi-dimensional culture systems have represented the most used method to study cell biology outside the body for over a century. Although they convey useful information, such systems may lose tissue-specific architecture, biomechanical effectors, and biochemical cues deriving from the native extracellular matrix, with significant alterations in several cellular functions and processes. Notably, the introduction of three-dimensional (3D) platforms that are able to re-create in vitro the structures of the native tissue, have overcome some of these issues, since they better mimic the in vivo milieu and reduce the gap between the cell culture ambient and the tissue environment. 3D culture systems are currently used in a broad range of studies, from cancer and stem cell biology, to drug testing and discovery. Here, we describe the mechanisms used by cells to perceive and respond to biomechanical cues and the main signaling pathways involved. We provide an overall perspective of the most recent 3D technologies. Given the breadth of the subject, we concentrate on the use of hydrogels, bioreactors, 3D printing and bioprinting, nanofiber-based scaffolds, and preparation of a decellularized bio-matrix. In addition, we report the possibility to combine the use of 3D cultures with functionalized nanoparticles to obtain highly predictive in vitro models for use in the nanomedicine field.

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“…The field of tissue engineering centers on development of tissues that are capable to regenerate and has a capacity to restore the damaged organs both structurally and functionally [1,2]. Scaffolds that are developed to serve this purpose should be able to provide cell attachment sites and allow cell proliferation and migration while maintaining its structural and mechanical integrity [2].…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Bioink from Xenogeneic Sources for 3D Bioprinting of Tissue Constructs

Yeleswarapu¹,

Chameettachal²,

Bera³

et al. 2020

Xenotransplantation - Comprehensive Study

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3D bioprinting brings new aspirations to the tissue engineering and regenerative medicine research community. However, despite its huge potential, its growth towards translation is severely impeded due to lack of suitable materials, technological barrier, and appropriate validation models. Recently, the use of decellularized extracellular matrices (dECM) from animal sources is gaining attention as printable bioink as it can provide a microenvironment close to the native tissue. Hence, it is worth exploring the use of xenogeneic dECM and its translation potential for human application. However, extensive studies on immunogenicity, safety-related issues, and animal welfare-related ethics are yet to be streamlined. In addition, the regulatory concerns need to be addressed with utmost priority in order to expedite the use of xenogeneic dECM bioink for 3D bioprinted implantable tissues for human welfare.

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Tissue engineering toward organ-specific regeneration and disease modeling

Cited by 65 publications

References 159 publications

Halogen-Mediated Partial Oxidation of Polyvinyl Alcohol for Tissue Engineering Purposes

Halogen-Mediated Partial Oxidation of Polyvinyl Alcohol for Tissue Engineering Purposes

Current Advances in 3D Tissue and Organ Reconstruction

Tissue-Specific Bioink from Xenogeneic Sources for 3D Bioprinting of Tissue Constructs

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