Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish

Dai, Yancen; Prithiviraj, N.; Gan, Jianhong; Zhang, Xin A.; Yan, Jizhou

doi:10.1038/srep38693

Cited by 11 publications

(11 citation statements)

References 34 publications

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“…Echinoderms, especially sea stars, have been used as model organisms for regenerative studies due to their amazing regenerative potential (Dai, Prithiviraj, Gan, Zhang, & Yan, 2016). The bio-molecules responsible for this property are predominantly the coelomocytes which are the immune cells present in the coelomic fluid (da Silva Laires, 2012).…”

Section: Discussionmentioning

confidence: 99%

AiP1, a protein from the coelomic fluid of sea star Astropecten indicus promotes wound healing and fibrinogenolysis in vitro

Baveja

Sarkar

Mondal

et al. 2019

JoBAZ

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Background: Marine animals are suspected to hold keys to counter several life-threatening diseases. Despite its vast biodiversity, the marine ecosystem is relatively unexplored for its therapeutic potential. This study was particularly aimed at finding a potent molecule against thrombosis from the coelomic fluid of a sea star (SCF). SCF is a reservoir of immunocytes, coelomocytes, cytokines, and other substances responsible for the exceptional regenerative potential and other bioactivities. Death due to thrombosis has increased significantly in the recent years. Healing of wounds caused due to accidents or surgical procedures is also of great significance, particularly for diabetic patients. Therefore, search for good thrombolytic and wound healing agents is being pursued by many research groups globally. Methods: SCF was fractionated using ion-exchange chromatography and size exclusion HPLC to yield a potent protein, AiP1. Proteolytic assays were performed using SDS-PAGE and zymography. Cell culture studies were performed as per standard protocols. Results: Purified protein, AiP1, showed fibrinogenolytic, fibrinolytic, and anti-platelet activities in a dose-dependent manner. Proteolytic activities of AiP1 were inhibited post treatment with EDTA, indicating its metalloproteinase nature. It was also devoid of cytotoxic, hemolytic, and phospholipase activities. Scratch wounds created on surface of HEK293 and HaCaT cells showed faster wound closure rates post treatment with AiP1 compared to untreated cells. Peptide mass fingerprinting showed the presence of vWFA-like domain in the central β sheet which may be responsible for its bioactivities. AiP1 was thus isolated and characterized for its thrombolytic and wound healing potential.Conclusion: Coelomic fluid of sea stars contains many bioactive compounds. One such compound is the protein AiP1 containing a vWFA-like domain. This protein showed significant wound healing and thrombolytic activities in vitro.

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Section: Discussionmentioning

confidence: 99%

AiP1, a protein from the coelomic fluid of sea star Astropecten indicus promotes wound healing and fibrinogenolysis in vitro

Baveja

Sarkar

Mondal

et al. 2019

JoBAZ

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“…37 Water is a critical factor that helps the wound healing of sea stars. 41 Similarly, the healing ability of boroxine systems also needs the stimulation of external water. The self-healing process of the P(4-VPBA 3 -co-PEGMA 1 )−boroxine thermoset was conducted by a cut-and-heal test (Figure 4a).…”

Section: Resultsmentioning

confidence: 99%

“…Water is a critical factor that helps the wound healing of sea stars . Similarly, the healing ability of boroxine systems also needs the stimulation of external water.…”

Section: Results and Discussionmentioning

confidence: 99%

Biomimetic Boroxine-Based Multifunctional Thermosets via One-Pot Synthesis

Yang

Guo

et al. 2020

ACS Appl. Mater. Interfaces

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Boroxine-based thermosets with remarkable mechanical tunability, self-healing ability, recyclability, and adhesive strength are of significant importance in various applications. However, complex multistep reactions are often required to prepare such thermosets. Herein, a facile one-pot approach to synthesize boroxine-based malleable thermosets is proposed. Random copolymers with pendant boronic acid groups were synthesized from alkenyl monomers containing boronic acids [4-vinylphenylboronic acid (4-VPBA), 3-vinylphenylboronic acid, or 3acrylamidophenylboronic acid] and octadecanoxy polyethylene glycol methacrylate. Then, the as-prepared copolymers were cured to form thermosets with boroxine bonds. The tensile strengths of the thermosets were tailored to range from 9.3 to 27.5 MPa by increasing the concentration of 4-VPBA. Moreover, because of the reversible nature of dynamic boroxine bonds (transformation between boroxines and boronic acids) induced by water, the thermosets exhibit remarkable self-healing efficiency (up to 99%), tunable mechanical properties, and excellent recyclability. Additionally, the thermosets also demonstrate superior adhesive strength (as high as 73.9 MPa) on different substrates.

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“…Increasingly, scientific works began to appear showing the effectiveness of the use of echinoderm extracts in the treatment of various diseases. In this regard, the research on the composition of metabolites of this type of animal is of great scientific interest [ 46 , 47 ].…”

Section: Introductionmentioning

confidence: 99%

Carotenoids from Starfish Patiria pectinifera: Therapeutic Activity in Models of Inflammatory Diseases

Popov,

Kozlovskaya,

Klimovich

et al. 2023

Marine Drugs

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The carotenoids mixture (MC) isolated from the starfish Patiria. pectinifera contains more than 50% astaxanthin, 4–6% each zeaxanthine and lutein, and less pharmacologically active components such as free fatty acids and their glycerides. Astaxanthin, the major component of MC, belongs to the xanthophyll class of carotenoids, and is well known for its antioxidant properties. In this work, in vitro and in vivo studies on the biological activity of MC were carried out. The complex was shown to exhibit anti-inflammatory, anti-allergic and cancer-preventive activity, without any toxicity at a dose of 500 mg/kg. MC effectively improves the clinical picture of the disease progressing, as well as normalizing the cytokine profile and the antioxidant defense system in the in vivo animal models of inflammatory diseases, namely: skin carcinogenesis, allergic contact dermatitis (ACD) and systemic inflammation (SI). In the skin carcinogenesis induced by 7,12-dimethylbenzanthracene, the incidence of papillomas was decreased 1.5 times; 1% MC ointment form in allergic contact dermatitis showed an 80% reduced severity of pathomorphological skin manifestations. Obtained results show that MC from starfish P. pectinifera is an effective remedy for the treatment and prevention of inflammatory processes.

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Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish

Cited by 11 publications

References 34 publications

AiP1, a protein from the coelomic fluid of sea star Astropecten indicus promotes wound healing and fibrinogenolysis in vitro

AiP1, a protein from the coelomic fluid of sea star Astropecten indicus promotes wound healing and fibrinogenolysis in vitro

Biomimetic Boroxine-Based Multifunctional Thermosets via One-Pot Synthesis

Carotenoids from Starfish Patiria pectinifera: Therapeutic Activity in Models of Inflammatory Diseases

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