Tissue hemostasis and chronic inflammation in colon biopsies of patients with inflammatory bowel disease

Reichman-Warmusz, Edyta; Kurek, Józef; Gabriel, Andrzej; Brzozowa, Marlena; Buła, Grzegorz; Helewski, Krzysztof; Domal-Kwiatkowska, Dorota; Gawrychowski, Jacek; Wojnicz, Romuald

doi:10.1016/j.prp.2012.06.005

Cited by 7 publications

(8 citation statements)

References 14 publications

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“…CD11b + cells were found in clusters near epithelial crypts in UC. Our findings are consistent with those of Oshitani et al who showed that CD11b + cells were upregulated in both inflamed and noninflamed colon and that the expression of CD11b correlated with the histologic degree of inflammation. , In addition, the proportion of CD3 + and CD45 + cells was lower in the colon of inflamed controls compared with healthy controls, which was demonstrated in our experimental results.…”

Section: Discussionsupporting

confidence: 93%

Exopolysaccharides from Lactobacillus plantarum NCU116 Facilitate Intestinal Homeostasis by Modulating Intestinal Epithelial Regeneration and Microbiota

Zhou

Zhang

et al. 2021

J. Agric. Food Chem.

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Regeneration of epithelia is crucial for maintaining the intestinal barrier and homeostasis. Our previous work showed that exopolysaccharides from Lactobacillus plantarum NCU116 (EPS116) regulated the barrier function and homeostasis of the intestine; however, the relevant mechanisms remain obscure. Therefore, we sought to explore the role of EPS116 in promoting intestinal epithelial regeneration. Our data showed that the administration of EPS116 markedly ameliorated inflammatory bowel disease-related phenotypes and promoted the regeneration of crypts in the colon of colitis mice. The results of immunofluorescence and reverse transcription-quantitative polymerase chain reaction experiments indicated that EPS116 strikingly increased the number of intestinal stem cells (ISCs) and the expression of differentiation markers for goblet cells, enterocytes, and enteroendocrine cells in the mouse colon. Intestinal microbiota analysis showed that EPS116 increased microbial populations associated with intestinal regeneration and glycan metabolism. Therefore, the present study revealed a novel model that EPS116 promoted the intestinal homeostasis through modulating the proliferation and differentiation of ISCs and altering the gut microbiota profile.

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Section: Discussionsupporting

confidence: 93%

Exopolysaccharides from Lactobacillus plantarum NCU116 Facilitate Intestinal Homeostasis by Modulating Intestinal Epithelial Regeneration and Microbiota

Zhou

Zhang

et al. 2021

J. Agric. Food Chem.

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“…This cell layer acts as a visual reminder of the physiological TF function as an intestinal 'haemostatic envelope', but also illustrates the interaction between factors of inflammation and coagulation. Our work also confirms the assumption of a macrophage contribution to intestinal TF expression, despite a previous IHC report on colonic TF where no association between CD68-staining and TF-positive mononuclear cells was found (15).…”

Section: Discussionsupporting

confidence: 89%

“…The inflammation-associated up-regulation of TF on pericryptal sheath cells and MNCs in UC is in accordance with previous IHC reports in IBD (14)(15)(16). Whether this means that TF has a direct role in IBD pathogenesis or is a parallel phenomenon is not clear as yet.…”

Section: Discussionsupporting

confidence: 89%

“…The main limitation of our study is the rather small patient groups, in particular, the group of inflammatory active IBD-albeit well in level with previous studies in this field (14)(15)(16). The low proportion of patients with inflammatory active colitis was an expected consequence of patient recruitment in connection to elective colonoscopy; still, this constitutes a limitation of the statistical basis.…”

Section: Discussionmentioning

confidence: 81%

“…Immunohistochemical studies on non-IBD-patients indicate a future role for TF in CRC prognostication (13). A limited number of in vivo studies addressing intestinal TF-expression in IBD have been performed (14)(15)(16), but, to our knowledge, the PSC-UC phenotype has never been addressed.…”

Section: Introductionmentioning

confidence: 99%

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Tissue factor in ulcerative colitis, with and without concomitant primary sclerosing cholangitis

Vessby

Lampinen

Åberg

et al. 2019

Upsala Journal of Medical Sciences

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Background: Ulcerative colitis (UC) in patients with the severe disease primary sclerosing cholangitis (PSC) constitutes a distinct clinical phenotype (PSC-UC) with a high incidence of colorectal cancer. Today, PSC-UC diagnosis is built on clinical observations only. Tissue factor (TF) has a potential use in UC diagnostics, and also in colorectal cancer prognostication. Here we evaluate TF expression in an inflammatory bowel disease (IBD) cohort, with special focus on differences between UC and PSC-UC patients. Materials and methods: Colonic biopsies from UC (n ¼ 23), PSC (n ¼ 24), and healthy controls (n ¼ 11) were stained for TF by immunohistochemistry. Mononuclear cell contribution to TF expression was verified using flow cytometry. Results: TF was distributed at three distinct colonic locations: in subepithelial pericryptal sheath cells, in mononuclear cells, and in the intestinal stroma. In contrast to UC-where inflammation was accompanied with TF up-regulation-PSC-UC activity remained low during inflammation. Stromal TF positivity was found exclusively in ongoing inflammation. Conclusion: Our study provides additional support for a divergent pathogenesis in PSC-UC, with an inflammatory environment that differs from classical UC. Stromal TF emerges as a new marker of colonic inflammation.

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Colonic Mucosal Immune Activity in Irritable Bowel Syndrome: Comparison with Healthy Controls and Patients with Ulcerative Colitis

et al. 2013

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Tissue hemostasis and chronic inflammation in colon biopsies of patients with inflammatory bowel disease

Cited by 7 publications

References 14 publications

Exopolysaccharides from Lactobacillus plantarum NCU116 Facilitate Intestinal Homeostasis by Modulating Intestinal Epithelial Regeneration and Microbiota

Exopolysaccharides from Lactobacillus plantarum NCU116 Facilitate Intestinal Homeostasis by Modulating Intestinal Epithelial Regeneration and Microbiota

Tissue factor in ulcerative colitis, with and without concomitant primary sclerosing cholangitis

Colonic Mucosal Immune Activity in Irritable Bowel Syndrome: Comparison with Healthy Controls and Patients with Ulcerative Colitis

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