Tissue Interactions with Dentin Specimens after Demineralization Using Tetracycline

Abstract

Greater cell attachment to demineralized dentin has been associated with subsequent development of a fiber attachment system. The purpose of this study was to evaluate tissue interactions to dentin demineralized with different concentrations of tetracycline solution. Dentin specimens were obtained from beneath root surfaces covered by periodontal ligament. Each rectangular specimen had a face of root surface dentin and an opposite surface of pulpal dentin. Experimental specimens were treated with a tetracycline solution of either 200 mg/cc or 100 mg/cc for five minutes. The remaining group of specimens served as untreated controls. Specimens were implanted transcutaneously into incisional wounds on the dorsal surface of rats with one end protruding through the skin. Four specimens in each group were available for examination one and ten days after implantation. Histologie and histometric analysis of both root and pulpal surface of implants included counts of adhering cells, assessment of implant length within the connective tissue, and evaluation of connective tissue fiber relationships. In each group, specimens became severely extruded between days one and ten, the number of attached cells decreased, and a fiber attachment system did not develop. Tetracycline‐treated surfaces had greater numbers of attached cells at both time points compared to untreated controls. No differences were discernible relating to different tetracycline concentrations. It was concluded that tetracycline‐demineralized dentin provided a substrate that increased cell attachment; however, this enhanced response did not result in a connective tissue attachment.

Subgingival instrumentation to remove simulated plaque in vitro: influence of operators’ experience and type of instrument

“…Supportive periodontal therapy (SPT) comprises the mechanical removal of supra-and subgingival plaque and calculus [1,2] to achieve biological compatibility of root surfaces, thereby allowing reattachment of adjacent tissues [3][4][5][6]. To do so, scaling is performed using hand [7,8], ultrasonic [7,9,10] or sonic instruments [11,12].…”

Comparison of the efficacy of ethylenediaminetetraacetic acid and tetracycline hydrochloride as root conditioning agents: An in vitro study

“…[19] In a study by Frantz and Polson, greater number of attached cells were found at 100 mg/ml of tetracycline HCl concentrations as compared to untreated controls. [24] Terranova et al also used the same concentration and found favorable results. [4] In case of EDTA, the concentration mostly used was in the form of a gel having 24% EDTA.…”

Comparative assessment of fluorosed and nonfluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after scaling and root planning and ethylenediaminetetraacetic acid root biomodification

“…As fibroblast adhesion and locomotion is facilitated by collagen, [64][65][66] and smear layer prevents it. The delicate balance of these two factors is vital to fibroblast attachment and both the factors determine the number of fibroblast attachment.…”