Tissue metabolic changes for effects of pirfenidone in rats of acute paraquat poisoning by GC-MS

Abstract

We developed a metabolomic method to evaluate the effect of pirfenidone on rats with acute paraquat (PQ) poisoning, through the analysis of various tissues (lung, liver, kidney, and heart), by gas chromatography-mass spectrometry (GC-MS). Thirty-eight rats were randomly divided into a control group, an acute PQ (20 mg kg) poisoning group, a pirfenidone (20 mg kg) treatment group, and a pirfenidone (40 mg kg) treatment group. Partial least squares-discriminate analysis (PLS-DA) revealed metabolic alterations in rat tissue samples from the two pirfenidone treatment groups after acute PQ poisoning. The PLS-DA 3D score chart showed that the rats in the acute PQ poisoning group were clearly distinguished from the rats in the control group. Also, the two pirfenidone treatment groups were distinguished from the acute PQ poisoning group and control group. Additionally, the pirfenidone (40 mg kg) treatment group was separated farther than the pirfenidone (20 mg kg) treatment group from the acute PQ poisoning group. Evaluation of the pathological changes in the rat tissues revealed that treatment with pirfenidone appeared to decrease pulmonary fibrosis in the acute PQ poisoning rats. The results indicate that pirfenidone induced beneficial metabolic alterations in the tissues of rats with acute PQ poisoning. Rats with acute PQ poisoning exhibited a certain reduction in biochemical indicators after treatment with pirfenidone, indicating that pirfenidone could protect liver and kidney function. Accordingly, the developed metabolomic approach proved to be useful to elucidate the effect of pirfenidone in rats of acute PQ poisoning.

Anti-inflammatory and anti-fibrotic treatment in a rodent model of acute lung injury induced by sulfur dioxide

“…IPF has some similarities with the acute effects of SO 2 -induced ALI, both appear to be driven by a mechanism that promote fibroblast recruitment and proliferation leading to a diffuse parenchymal lung disease, resulting in loss of lung function [15,34]. PFD-treatment has shown to have protective effects in other experimental lung disease-models induced by e.g., paraquat, bleomycin and allergen-induced asthma [30,[37][38][39]. In addition to reducing pulmonary fibrosis, PFD-treatment has also been shown to attenuate fibrosis in the liver, heart and kidney [34].…”

Metabolic and transcriptional changes in seminal plasma of asthenozoospermia patients

“…Currently, metabolomics is generally applied to fields such as pharmacology and toxicology, disease pathogenesis, clinical diagnosis, and plant growth (Bao et al, ; Chen et al, ,b; Jiang et al, ; Ma et al, ). In particular, targeted metabolomics based on triple quadrupole MS has the capacity to perform a robust and precise metabolome analysis owing to its excellent quantitation ability (Chen et al, ).…”

Ameliorative Activity of Ascorbic Acid (Aa) on Paraquat (Pq) Induced Serum Biochemical Alterations in Experimental Rats

“…A total of 48 male albino Wistar rats were randomly divided into four (4) groups consisting of twelve (12) animals in each. Group 1 -Control Group 2 -PQ (@ 40 mg/kg b.wt) Group 3 -AA (@250 mg/kg b.wt) Group 4 -PQ (@40 mg/kg b.wt) + AA (@ 250 mg/kg b.wt)…”