Tissue-Mimicking Phantom Useful in Simulating Laser Light-Tissue Interactions

Abstract

Significant methodological advancements in preclinical experiments using Schlieren imaging, in vitro cell lines, ex vivo tissues, human cadaver tissues, and tissue-mimicking (TM) phantoms have been tremendously helpful in outlining actual skin reactions to laser or light energy. Polyacrylamide hydrogel TM phantom containing bovine serum albumin, with some modifications, depending on the study design, can be used to simulate laser-or light-induced tissue reactions. Although experiments in TM phantoms do not mirror in vivo laser light-induced skin reactions exactly, mainly due to the lack of cellular components and adnexal structures, TM phantoms are useful in determining which devices and settings offer better treatment results and safer delivery of laser energy to experimental and clinical subjects.