Tissue proteome analysis revealed an association between cancer, immune system response, and the idiopathic granulomatous mastitis

Albayrak, Merve Gülsen Bal; Şimşek, Turgay; Kasap, Murat; Akpınar, Gürler; Cantürk, Nuh Zafer; Güler, Sertaç Ata

doi:10.1007/s12032-022-01845-2

Cited by 13 publications

(6 citation statements)

References 45 publications

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“…Researchers have also found that LXB4 boosts memory B cells via COX2 [ 37 ]. Although many studies [ 38 , 39 ] have linked NPM to immunity, its precise mechanism of action remains unknown. In summary, screening differential lipids and pathway analysis may provide a new direction for studying the immune-related mechanisms of NPM.…”

Section: Discussionmentioning

confidence: 99%

LC/MS-based untargeted lipidomics reveals lipid signatures of nonpuerperal mastitis

Chen

Shijun

et al. 2023

Lipids Health Dis

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Background Nonpuerperal mastitis (NPM) is a disease that presents with redness, swelling, heat, and pain during nonlactation and can often be confused with breast cancer. The etiology of NPM remains elusive; however, emerging clinical evidence suggests a potential involvement of lipid metabolism. Method Liquid chromatography‒mass spectrometry (LC/MS)-based untargeted lipidomics analysis combined with multivariate statistics was performed to investigate the NPM lipid change in breast tissue. Twenty patients with NPM and 10 controls were enrolled in this study. Results The results revealed significant differences in lipidomics profiles, and a total of 16 subclasses with 14,012 different lipids were identified in positive and negative ion modes. Among these lipids, triglycerides (TGs), phosphatidylethanolamines (PEs) and cardiolipins (CLs) were the top three lipid components between the NPM and control groups. Subsequently, a total of 35 lipids were subjected to screening as potential biomarkers, and the chosen lipid biomarkers exhibited enhanced discriminatory capability between the two groups. Furthermore, pathway analysis elucidated that the aforementioned alterations in lipids were primarily associated with the arachidonic acid metabolic pathway. The correlation between distinct lipid populations and clinical phenotypes was assessed through weighted gene coexpression network analysis (WGCNA). Conclusions This study demonstrates that untargeted lipidomics assays conducted on breast tissue samples from patients with NPM exhibit noteworthy alterations in lipidomes. The findings of this study highlight the substantial involvement of arachidonic acid metabolism in lipid metabolism within the context of NPM. Consequently, this study offers valuable insights that can contribute to a more comprehensive comprehension of NPM in subsequent investigations. Trial registration Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine (Number: 2019-702-57; Date: July 2019).

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Section: Discussionmentioning

confidence: 99%

LC/MS-based untargeted lipidomics reveals lipid signatures of nonpuerperal mastitis

Chen

Shijun

et al. 2023

Lipids Health Dis

View full text Add to dashboard Cite

show abstract

“…Researchers have also found that LXB4 boosts memory B cells via COX2 [32]. Many studies [33,34] have linked NPM to immunity, but its precise mechanism of action remains unknown. In summary, screening differential lipid metabolites and pathway analysis may provide new direction for studying immune-related mechanisms of NPM.…”

Section: Discussionmentioning

confidence: 99%

LC/MS based untargeted lipidomics reveals lipid signatures of Non-Puerperal Mastitis

Chen

Shijun

et al. 2023

Preprint

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Background: Non-Puerperal Mastitis (NPM) is a type of disease that occurs during non-lactation accompanied by redness, swelling, heat and pain which is easily confused with breast cancer. The etiology of NPM is not yet clear. There is growing evidence from research showing that lipid metabolism may play an important role in NPM. This study aimed to use lipidomics to provide evidence for the etiology and potential therapeutic approaches for NPM. Method: Liquid chromatography mass spectrometry (LC/MS)-based untargeted lipidomics analysis combined with multivariate statistics were performed on investigating the NPM lipid change in breast tissue. Results: The results revealed significant differences in lipidomics profiles and a total of 16 subclasses with 14,012 different lipids were identified in positive and negative ion modes. Among these lipids, Triradylcglycerols (TAGs), Glycerophosphoethanolamines (PEs) and Glycerophosphoglycerophosphoglycerols (CLs)are top three lipid components between the NPM and control groups. Subsequently, 35 significantly different lipid metabolites were screened as potential biomarkers, such as 15(S)-Hydroxyeicosatrienoic acid, Lipoxin A4 (LXA4), Lipoxin B4 (LXB4) and LysoPC(16:0), and they showed good diagnostic performance. Detailed pathway analysis revealed that these dysregulated lipids are closely related to arachidonic acid metabolism, glycerophospholipid metabolism. Conclusions: This study showed that NPM patients' breast tissue exhibited altered lipid groups. In addition, the lipid metabolites screened and the pathways identified in this study may be potential targets for future studies of the pathogenesis of NPM. Trial registration: Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine (Number: 2019-702-57; Date: July 2019).

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“…In some cases, multiple biopsies may be necessary for clinical differential diagnosis. Furthermore, a comprehensive analysis of the tissue proteome in GLM patients has identi ed 16 immune-related proteins and 17 proteins associated with cancer metabolism, highlighting the complex molecular relationship between GLM and BC 27 .Similarly, based on the literature review, Aksan et al have identi ed serum miR-21 and PTEN levels as potential non-invasive biomarkers for distinguishing between GLM and BC 28 . No other studies have investigated serum biomarkers speci c to GLM.…”

Section: Discussionmentioning

confidence: 99%

Circulating miR-451a, miR-5571-3p, CLN6, HSD11B1 and PDE4 levels in differential diagnosis of Granulomatous lobular mastitis, breast cancer and breast fibroadenoma

LING,

WU,

DENG

et al. 2023

Preprint

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This study aimed to investigate the diagnostic value of miR-451a, miR-5571-3p, CLN6, HSD11B1, and PDE4 as potential biomarkers for distinguishing patients with Granulomatous lobular mastitis (GLM), breast cancer (BC), and breast fibroadenoma. A total of 55 participants were included in each group. The serum expressions of miR-451a and miR-5571-3p were significantly higher in the GLM (0.95 ± 0.47, 7.20 ± 3.99) and control groups (0.21 ± 0.13, 1.74 ± 1.49) than in the BC group (p < 0.01). Meanwhile, the expression of miR-451a and miR-5571-3p in GLM was significantly higher than in the control group (0.21 ± 0.13, 1.74 ± 1.49; p < 0.001). The levels of CLN6 in the GLM(1.37 ± 0.87) and BC((1.57 ± 0.84) groups were higher than those in the control group (p < 0.001). Furthermore, HSD11B1 and PDE4 expressions were significantly increased in the BC (1.92 ± 1.14, 1.54 ± 0.88) and GLM (1.07 ± 0.70, 0.85 ± 0.51) groups compared to the control group (p < 0.01), with higher levels observed in the BC group than in the GLM group (p < 0.01). Taken together, these findings suggest that serum miR-451a, miR-5571-3p, and CLN6 may serve as noninvasive biomarkers for differentiating GLM, BC, and breast fibroadenoma. This study provides a basis for future investigations in the differential diagnosis of these breast diseases.

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Tissue proteome analysis revealed an association between cancer, immune system response, and the idiopathic granulomatous mastitis

Cited by 13 publications

References 45 publications

LC/MS-based untargeted lipidomics reveals lipid signatures of nonpuerperal mastitis

LC/MS-based untargeted lipidomics reveals lipid signatures of nonpuerperal mastitis

LC/MS based untargeted lipidomics reveals lipid signatures of Non-Puerperal Mastitis

Circulating miR-451a, miR-5571-3p, CLN6, HSD11B1 and PDE4 levels in differential diagnosis of Granulomatous lobular mastitis, breast cancer and breast fibroadenoma

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