Tissue Regeneration and Physiological Functional Recovery in Dental and Craniofacial Fields

Ouchi, Takehito; Nakagawa, Taneaki

doi:10.3390/biom11111644

Cited by 3 publications

(3 citation statements)

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“…It has been discovered that the amelogenin protein can self-assemble into nanospheres and it has regulatory effects on the bio-fabrication of enamel in mammalian teeth. Enamel biomineralization is negatively impacted by mutations that result in modifications to the amelogenin protein’s functional domains [ 75 , 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

Applications of Biotechnology to the Craniofacial Complex: A Critical Review

et al. 2022

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Background: Biotechnology shows a promising future in bridging the gap between biomedical basic sciences and clinical craniofacial practice. The purpose of the present review is to investigate the applications of biotechnology in the craniofacial complex. Methods: This critical review was conducted by using the following keywords in the search strategy: “biotechnology”, “bioengineering”, “craniofacial”, “stem cells”, “scaffolds”, “biomarkers”, and ”tissue regeneration”. The databases used for the electronic search were the Cochrane Library, Medline (PubMed), and Scopus. The search was conducted for studies published before June 2022. Results: The applications of biotechnology are numerous and provide clinicians with the great benefit of understanding the etiology of dentofacial deformities, as well as treating the defected areas. Research has been focused on craniofacial tissue regeneration with the use of stem cells and scaffolds, as well as in bioinformatics with the investigation of growth factors and biomarkers capable of providing evidence for craniofacial growth and development. This review presents the biotechnological opportunities in the fields related to the craniofacial complex and attempts to answer a series of questions that may be of interest to the reader. Conclusions: Biotechnology seems to offer a bright future ahead, improving and modernizing the clinical management of cranio-dento-facial diseases. Extensive research is needed as human studies on this subject are few and have controversial results.

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Section: Discussionmentioning

confidence: 99%

Applications of Biotechnology to the Craniofacial Complex: A Critical Review

et al. 2022

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“…The renewal of dental tissue has long posed a significant challenge. Many treatments have been attempted, including tissue transplantation and the use of biomaterials and growth factors; however, none have proved satisfactory (1)(2)(3). The use of cell-based treatments has been recently proposed and has provided insights into the processes of dental tissue regenerative mechanisms and the potential for effective therapy (4,5).…”

Section: Introductionmentioning

confidence: 99%

Pluripotency of periodontal ligament and dental pulp cells is induced by intercellular communication via the CDX2/Oct-4/Sox2 pathway

Wei¹,

Peng²

2022

Ann Transl Med

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Background: Intercellular communication in the environments of mature or aged cells can restore and regenerate their function and promote the expression of pluripotency markers. The regeneration of dental tissue is stimulated by periodontal ligament cells (PDLCs) and dental pulp cells (DPCs). However, the communication networks between the cells and their microenvironments are poorly understood.Methods: In this study, gene expression was analyzed by polymerase chain reaction, and chromatin immunoprecipitation assays, dual-luciferase assays, and electrophoretic mobility shift assays were used to analyze the signaling pathways associated with pluripotency after the knockdown or overexpression of caudal-type homeobox transcription factor 2 (CDX2).Results: Elevated levels of SRY-box transcription factor 2 (Sox2) and octamer-binding transcription factor 4 (Oct-4) were observed in the co-culture system, while the levels of CDX2 were significantly reduced. The overexpression of CDX2 promoted cell apoptosis and reduced the synthesis stage of the cell cycle. CDX2 was shown to bind directly to the promoter regions of Sox2 and Oct-4. The silencing of CDX2 promoted calcium deposition, adipogenic differentiation, and elevated alkaline phosphatase (ALP) activity in the DPCs.Conclusions: These findings demonstrate the enhancement of DPC and PDLC pluripotency by intercellular communication. CDX2 plays a significant part in the regulation of DPC and PDLC pluripotency via its regulation of Oct-4 and Sox2 expression.

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“…To prevent this phenomenon, intracellular signaling control, intercellular communication, genetic regulation, and functional regenerative medicine based on tissue engineering have been considered [ 3 ]. In a previous edition titled “Tissue Regeneration and Physiological Functional Recovery in Dental and Craniofacial Fields”, we discussed the potential of oral cells in tissue regeneration and functional recovery [ 4 ]. In the second edition, several studies were discussed and researchers showed the other potentials of oral and cranial stem cells to use in advanced therapeutic strategies.…”

mentioning

confidence: 99%