Tissue response to a newly developed calcium phosphate cement containing succinic acid and carboxymethyl‐chitin

Abstract

We developed a new calcium phosphate cement containing succinic acid and carboxymethyl-chitin in the liquid component. In this study, the biocompatibility and osteoconductivity of this new cement were investigated. After mixing, cement in putty form was implanted immediately between the periosteum and parietal bone and in the subcutaneous tissues of rats. In control cement, distilled water was used instead of the liquid component. In addition to histological evaluations, analyses with X-ray diffraction and Fourier transform infrared were performed for the subcutaneously implanted cements. Histological examination showed slight inflammation around the new cement on the bone and in the subcutaneous tissue at 1 week after surgery. At 2 weeks, the cement was partially bound to the parietal bone. The extent of the surface of the new cement directly in contact with the bone increased with time, and most of the undersurface of the new cement bound to the host parietal bone by 8 weeks. Analysis by X-ray diffraction showed that the new cement in the subcutaneous tissue was transformed into hydroxyapatite by 8 weeks. These results indicate that this new calcium phosphate cement is useful as a bone substitute material.

Polymeric additives to enhance the functional properties of calcium phosphate cements

“…83 Incorporating chitin also increases compressive strength from 23 MPa in the control to 33 MPa in the composite material. 83 However, a high chitin content is detrimental to CPC resorption under in vivo conditions. 136 …”

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

“…Specifically, polysaccharides [97,108,[335][336][337][338], gelatin [278,[339][340][341][342][343][344][345] and polyacrylic acid [346][347][348] are of an interest due to their biocompatibility and good rheological properties. Only small amounts (a few weight %) are needed to dramatically increase the viscosity of the cement pastes.…”

“…In order to maintain a good cohesion and reduce filter pressing, calcium orthophosphate cements need to be more viscous (hence, less injectable) [197,198]. For example, calcium orthophosphate cements might be modified by addition of polysaccharides [97,108,[335][336][337][338] and/or gelatin [278,[339][340][341][342][343][344].…”

Self-Setting Calcium Orthophosphate Formulations

“…Specifically, polysaccharides [120,132,[380][381][382][383], gelatin [317,[384][385][386][387][388][389][390] and polyacrylic acid [391][392][393] are of an interest due to their biocompatibility and good rheological properties. Only small amounts (a few weight %) are needed to dramatically increase the viscosity of the pastes.…”

“…In order to maintain a good cohesion and reduce filter pressing, the calcium orthophosphate formulations need to be more viscous (hence, less injectable) [4,5]. For example, they might be modified by addition of polysaccharides [120,132,[380][381][382][383] and/or gelatin [317,[384][385][386][387][388][389].…”