Tissue response to an experimental calcium hydroxide‐based endodontic sealer: a quantitative study in subcutaneous connective tissue of the rat

Abstract

Silicone tubes containing a freshly mixed experimental Sealapex, in which titanium dioxide was removed from the original formula, were implanted in the dorsal subcutaneous connective tissue of the rat. Solid silicone rods of the same size as the tubes were also implanted and used as inert controls. The tissue reaction to both these materials was histometrically and quantitatively studied under light microscopy and by elemental electron microprobe analysis. After 7, 30 and 90 d of implantation, different grades of tissue reaction to the tested materials were recorded. A granulomatous tissue containing numerous foreign-body giant cells and macrophages with engulfed particles in their cytoplasms was initially detected in contact with the test material. In addition, many fibroblasts and newly formed vessels were also observed at these areas. The results of this study revealed that these reactions increased progressively at the 30- and 90-d observations. The electron microprobe analysis of the granulomatous tissues showed the presence of heavy components of the experimental material.

Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers

“…21 His pioneering research, which aimed to evaluate the reaction of the subcutaneous connective tissue of rats to implanted polyethylene tubes, became a commonly used preliminary method to evaluate biocompatibility. 22,23 Here the reactions to empty tubes were similar to those that were previously reported. 16,24 Previous studies reported that a glycol methacrylate technique was an excellent alternative for the evaluation of biocompatibility of endodontic sealers; this technique is easy to execute and reproducible and thereby provides a better definition of the degree of the inflammatory process.…”

Reação do tecido conjuntivo subcutâneo de ratos ao implante de Hipoglós®

“…As avaliações quantitativas das células inflamatórias (linfócitos, plasmócitos, polimorfonucleares neutrófilos), macrófagos e células gigantes foram realizadas em 10 áreas separadas, de cortes com magnificação de 400X 8 . O valor médio de cada grupo foi obtido pela soma das células computadas nessas 10 áreas diferentes.…”

Materiais obturadores de canais radiculares de dentes decíduos: avaliação da atividade antibacteriana in vitro e da compatibilidade tecidual in vivo

“…Os implantes em tecido conjuntivo subcutâneo são usados para avaliar a reposta biológica deste tecido frente ao material implantado, ao nível macro e microscópico (ISO 10993-6, 1994), podendo ser utilizados como fonte preliminar de informações com respeito à compatibilidade do material com a atividade biológica dos tecidos ao seu redor (Zmener et al, 1988;Zmener et al, 1990;Bilginer et al, 1997;Costa et al, 2000;Kaplan et al, 2003;Ozbas et al, 2003;Shimizu et al, 2004;Yaltirik et al, 2004;Zmener et al, 2004;Souza et al, 2006;Sumer et al, 2006;Batista et al, 2007;Silva, 2007;Zafalon et al, 2007 Os testes comprobatórios (no caso de se estar avaliando materiais usados em endodontia) são os que avaliam a resposta do tecido pulpar, tecidos apicais e periapicais frente ao material (Stanley, 1992 …”

“…Na literatura, observa-se que os parâmetros avaliados pelos diferentes autores (Zmener et al, 1988;Zmener et al, 1990;Molloy et al, 1992;Economides et al, 1995;Bilginer et al, 1997;Kaplan et al, 2003;Ozbas et al, 2003;Shimizu et al, 2004;Yaltirik et al, 2004;Zmener, 2004;Souza et al, 2006;Sumer et al, 2006;Batista et al, 2007;Onay et al, 2007;Zafalon et al, 2007) são praticamente os mesmos e incluem tipo e quantidade de células inflamatórias, fibrosamento, área e espessura do tecido reacional, necrose e fenômenos vasculares. Entretanto, existe ausência de consenso com relação à forma de se realizar a avaliação da resposta biológica dos tecidos ao material implantado.…”