Tissue Specific and Subcellular Distribution of Creatine Kinase Isoenzymes

Neumeier, Daniel; Jockers-Wretou, Evangelia

doi:10.1007/978-3-642-68041-0_4

Cited by 32 publications

(37 citation statements)

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“…CK is thus thought to fuel highly energy-demanding processes, such as sodium retention, cardiovascular contractility, and remodeling of arteries. [5][6][7][8][9][10] In accord with this, serum CK was found to be the main predictor of blood pressure in the general population. 6 This was proposed to be because of high tissue CK, primarily the resistance artery CK-BB isoenzyme rapidly regenerating ATP for vascular contractility.…”

Section: +supporting

confidence: 52%

“…The total transcript level per tissue is set to 100% (total mean normalized CK copy numbers, respectively, are 331.3 for skeletal muscle, 85.9 for heart, 10.1 for renal artery, 4.8 for small intestine, 14.8 for colon, and 12.5 for brain tissue, in accord with tissue differences in total CK protein levels as previously reported). 9,16 Individual transcript fractions were calculated and marked as indicated. The results show CKM/CKMT2 transcription mainly in striated muscle, and CKB/CKMT1 transcription mainly in smooth muscle and other tissue.…”

Section: Discussionmentioning

confidence: 99%

“…[5][6][7]23 ATP is required for each actin-myosin complex formed. [5][6][7][8][9][10][11] Vascular smooth muscle contraction is thought to consist of a fast, force-generating component at relatively high-energy costs, and a slow, tonic maintenance of tension, for which ADP is required. [5][6][7]19,22,23 If, because of greater CK activity, ADP levels at the contractile proteins do not achieve the level required for tonic maintenance of tension, then the smooth muscle tension response could be altered, leading to excessive contractility.…”

Section: Discussionmentioning

confidence: 99%

“…The enzymatic functional form can be either a homodimer (BB and MM) or a heterodimer (MB) thus creating 3 cytosolic isoenzymes. 5,9,10,12 CK is also present in the mitochondrion where it facilitates the formation of creatine phosphate that is transported by CK to subcellular locations of high-energy demands. 12,13 Two mitochondrial CK isoenzymes, an ubiquitous and a sarcomeric form, are encoded by, respectively, the CKMT1 gene on chromosome 15q15 and the CKMT2 gene on chromosome 5q13.3.…”

Section: Ck Isoenzyme Cdnamentioning

confidence: 99%

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Resistance Artery Creatine Kinase mRNA and Blood Pressure in Humans

et al. 2014

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Protocols were in accord with institutional guidelines and were approved by the local institutional review board. All participants gave written informed consent. Consecutive, self-defined white and African-Dutch women undergoing an abdominal procedure for fibroid enucleation or hysterectomy for fibroids were eligible for inclusion.Abstract-Hypertension remains the main risk factor for cardiovascular death. Environmental and biological factors are known to contribute to the condition, and circulating creatine kinase was reported to be the main predictor of blood pressure in the general population. This was proposed to be because of high resistance artery creatine kinase-BB rapidly regenerating ATP for vascular contractility. Therefore, we assessed whether creatine kinase isoenzyme mRNA levels in human resistance arteries are associated with blood pressure. We isolated resistance-sized arteries from omental fat donated by consecutive women undergoing uterine fibroid surgery. Blood pressure was measured in the sitting position. Vessels of 13 women were included, 6 normotensive and 7 hypertensive, mean age 42.9 years (SE, 1.6) and mean systolic/ diastolic blood pressure, 144.8 (8.0)/86.5 (4.3) mm Hg. Arteriolar creatine kinase isoenzyme mRNA was assessed using quantitative real-time polymerase chain reaction. Normalized creatine kinase B mRNA copy numbers, ranging from 5.2 to 24.4 (mean, 15.0; SE, 1.9), showed a near-perfect correlation with diastolic blood pressure (correlation coefficient, 0.9; 95% confidence interval, 0.6-1.0) and were well correlated with systolic blood pressure, with a 90% relative increase in resistance artery creatine kinase B mRNA in hypertensives compared with normotensives, normalized copy numbers were, respectively, 19.3 (SE, 2.0) versus 10.1 (SE, 2.1), P=0.0045. To our knowledge, this is the first direct evidence suggesting that resistance artery creatine kinase mRNA expression levels concur with blood pressure levels, almost doubling with hypertension. These findings add to the evidence that creatine kinase might be involved in the vasculature's pressor responses. (Hypertension. 2014;63:68-73.)

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Section: +supporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Ck Isoenzyme Cdnamentioning

confidence: 99%

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Resistance Artery Creatine Kinase mRNA and Blood Pressure in Humans

et al. 2014

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“…It is known that CK-MM and CK-MB are mainly present in the skeletal and heart muscles, respectively (24). CK-BB is distributed mainly in the brain but also in the prostate gland, urinary bladder and gastrointestinal tract in adults (13). Interestingly, it was found that CK-BB in the skeletal muscle is replaced through CK-MB by CK-MM during human embryonic and fetal development (4,8,25).…”

mentioning

confidence: 99%

Immunohistochemical study on the distribution of creatine kinase subunits in early human embryos.

Oguni

Setogawa

Tanaka

et al. 1992

Acta Histochem. Cytochem

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Creatine kinase (CK) is a dimer composed of two immunologically distinct subunits, CK-M and CK-B, and takes part in the production of adenosine triphosphate.In the present study, the distribution of CK in the muscles and the nervous system was almost identical to that reported in older fetuses. However, in the epithelia of trachea and esophagus, CK-B immunoreactivity was apparent only in young embryos and decreased with the age of the embryo, while CK-M immunoreactivity was absent or weak in early stages and increased thereafter. In the surface ectoderm, CK-B was not detected immunohistochemically while CK-M was evident in early embryos and decreased as the embryonic stage advanced. Among the ectodermal derivatives, the lens placode was immunoreactive only to CK-M antibody at stage 13. This immunoreactivity decreased and finally disappeared along with the formation of the lens vesicle. In the urogenital system, mesonephric tubules were immunoreactive to both CK-B and CK-M antibodies, while mesonephric ducts were immunoreactive only to the CK-M antibody. Mesonephric glomeruli were not immunostained with these antibodies.These results in the urogenital system were found in all embryos examined. The present results suggest that the distribution of CK subunits in several organs of earlier embryos differs not only from that in adults, but also from that in older embryos and fetuses.

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Significance of elevated MB creatine kinase in patients after cardiac catheterization

Rosenblum

Ludbrook

Jaffe

1984

Cathet. Cardiovasc. Diagn.

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To clarify the etiology of elevations in plasma MB creatine kinase (CK) in patients after cardiac catheterization, we studied 32 consecutive patients undergoing cardiac catheterization and coronary arteriography. Total CK and MB CK were within the normal range in all patients prior to catheterization. Total CK activity rose from a mean of 61.46 +/- 33.8 IU/1 (SD) to 141 +/- 105 in the first sample after catheterization (p less than .005) and 121.6 +/- 92.4 in the second catheterization sample (p less than .0005). The MB CK activity also rose from a mean of 3.2 +/- 1.6 IU/1 prior to catheterization to a maximum value of 5.0 +/- 2.9. The mean increase in MB CK, though statistically significant (p less than .005), was only 1.8 IU/1. Only one patient's value for MB CK rose to outside of the normal range (greater than 12) likely due to cardiac injury. Thus, our data document that marked elevations in MB CK after cardiac catheterization are unusual. They likely represent cardiac muscle injury rather than MB CK released due to skeletal muscle injury induced by the catheterization itself.

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Tissue Specific and Subcellular Distribution of Creatine Kinase Isoenzymes

Cited by 32 publications

References 0 publications

Resistance Artery Creatine Kinase mRNA and Blood Pressure in Humans

Resistance Artery Creatine Kinase mRNA and Blood Pressure in Humans

Immunohistochemical study on the distribution of creatine kinase subunits in early human embryos.

Significance of elevated MB creatine kinase in patients after cardiac catheterization

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