Tissue-specific decellularized endometrial substratum mimicking different physiological conditions influences in vitro embryo development in a rabbit model

Campo, Hannes; García‐Domínguez, Ximo; López-Martínez, Sara; Faus, Amparo; Antón, José Salvador Vicente; Marco‐Jiménez, Francisco; Cervelló, Irene

doi:10.1016/j.actbio.2019.03.004

Cited by 56 publications

(52 citation statements)

References 70 publications

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“…We previously reported that tissue-specific ECM-derived coatings obtained from rabbit endometrium at different stages of proliferation influence the in vitro growth of rabbit embryos (Campo et al, 2019). In the present study, decellularized endometrium from porcine uterus was used to create an endometrial extracellular matrix (EndoECM) hydrogel.…”

Section: Introductionmentioning

confidence: 95%

“…Then, decellularized (DC) horns stored at −80 • C were cut transversally into 1 mm thick ring-shaped disks. The endometrial fraction was isolated via microdissection under a stereomicroscope (SMZ800, Nikon) by cutting the luminal side of the inner circular myometrial layer, which appears as a dense line in both No-DC and DC horns (Figure 2; Campo et al, 2019). The remaining myometrial fraction was kept as a control to verify the correct isolation of pure endometrium and the presence of tissuespecific components during proteomic analysis.…”

Section: Porcine Uterus Decellularization and Endometrial Isolationmentioning

confidence: 99%

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A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

López-Martínez

Campo

Miguel-Gómez

et al. 2021

Front. Bioeng. Biotechnol.

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Decellularization techniques support the creation of biocompatible extracellular matrix hydrogels, providing tissue-specific environments for both in vitro cell culture and in vivo tissue regeneration. We obtained endometrium derived from porcine decellularized uteri to create endometrial extracellular matrix (EndoECM) hydrogels. After decellularization and detergent removal, we investigated the physicochemical features of the EndoECM, including gelation kinetics, ultrastructure, and proteomic profile. The matrisome showed conservation of structural and tissue-specific components with low amounts of immunoreactive molecules. EndoECM supported in vitro culture of human endometrial cells in two- and three-dimensional conditions and improved proliferation of endometrial stem cells with respect to collagen and Matrigel. Further, we developed a three-dimensional endometrium-like co-culture system of epithelial and stromal cells from different origins. Endometrial co-cultures remained viable and showed significant remodeling. Finally, EndoECM was injected subcutaneously in immunocompetent mice in a preliminary study to test a possible hypoimmunogenic reaction. Biomimetic endometrial milieus offer new strategies in reproductive techniques and endometrial repair and our findings demonstrate that EndoECM has potential for in vitro endometrial culture and as treatment for endometrial pathologies.

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Section: Introductionmentioning

confidence: 95%

Section: Porcine Uterus Decellularization and Endometrial Isolationmentioning

confidence: 99%

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

López-Martínez

Campo

Miguel-Gómez

et al. 2021

Front. Bioeng. Biotechnol.

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“…[7,8] Also, the ECM has been revealed to potentially release growth factors and cytokines which can induce and regulate growth, proliferation, and differentiation of cells. [9] Decellularized scaffolds have been widely utilized for TE, such as blood vessels, [10] urinary bladder, [11] and skin [12] in clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study

Weng

Xie

et al. 2021

Preprint

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Background: Advances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM).Methods: 24 male New Zealand white rabbits were used in this experimental study. The rabbit thyroid glands were decellularized by immersion/agitation decellularization protocol. The 3D thyroid decellularization scaffolds were tested with histological and immunostaining analyses, scanning electron microscopy, DNA quantification, mechanical properties test, cytokine assay and cytotoxicity assays. Meanwhile, the decellularization scaffold were seeded with human thyroid follicular cells, cell proliferation and thyroid peroxidase were determined to explore the biocompatibility in vitro.Results: Notably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression.Conclusions: The adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.

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“…Extensive reviews have reported that ART-stressors during in vitro fertilisation (IVF) and in vitro culture (IVC) may not only impair embryo development in the laboratory, but can also have downstream effects after the embryo transfer across gestation and after birth [ 7 , 10 , 11 , 12 , 13 ]. One potential strategy to attenuate the suboptimal conditions is the use of oviductal and uterine secretions and/or cells, which results in embryos closer to those conceived naturally [ 14 , 15 , 16 , 17 ]. As the increasing use of ARTs continues to spread worldwide, mimicking in vivo conditions is especially important to guarantee the safety of ART procedures, but some of them are extremely contradictory with this concept, such as embryo cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer

García‐Domínguez

Diretto

Frusciante

et al. 2020

IJMS

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Although assisted reproduction technologies (ARTs) are recognised as safe, and most of the offspring seem apparently healthy, there is clear evidence that ARTs are associated with changes in the embryo’s developmental trajectory, which incur physiological consequences during the prenatal and postnatal stages of life. The present study aimed to address the influence of early (day-3 embryos) embryo transfer and cryopreservation on embryo survival, size, and metabolome at the preimplantation stage (day-6 embryos). To this end, fresh-transferred (FT) and vitrified-transferred (VT) embryos were compared using naturally-conceived (NC) embryos as a control reference. The results show that as in vitro manipulation was increased (NC < FT < VT), both embryo survival rate (0.91 ± 0.02, 0.78 ± 0.05 and 0.63 ± 0.05, for NC, FT, and VT groups, respectively) and embryo size (3.21 ± 0.49 mm, 2.15 ± 0.51 mm, 1.76 ± 0.46 mm of diameter for NC, FT, and VT groups, respectively) were significantly decreased. Moreover, an unbiased metabolomics analysis showed overall down-accumulation in 40 metabolites among the three experimental groups, with embryo transfer and embryo cryopreservation procedures both exerting a cumulative effect. In this regard, targeted metabolomics findings revealed a significant reduction in some metabolites involved in metabolic pathways, such as the Krebs cycle, amino acids, unsaturated fatty acids, and arachidonic acid metabolisms. Altogether, these findings highlight a synergistic effect between the embryo transfer and vitrification procedures in preimplantation embryos. However, the ex vivo manipulation during embryo transfer seemed to be the major trigger of the embryonic changes, as the deviations added by the vitrification process were relatively smaller.

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Tissue-specific decellularized endometrial substratum mimicking different physiological conditions influences in vitro embryo development in a rabbit model

Cited by 56 publications

References 70 publications

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study

Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer

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Tissue-specific decellularized endometrial substratum mimicking different physiological conditions influences in vitro embryo development in a rabbit model

Cited by 56 publications

References 70 publications

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a&nbsp;preliminary&nbsp;in&nbsp;vitro&nbsp;study

Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer

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Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study