Tissue-Specific Expression and Anaerobically Induced Posttranscriptional Modulation of Sucrose Synthase Genes in <i>Sorghum bicolor</i> M.

Chourey, Prem S.; Taliercio, Earl; Kane, E. J.

doi:10.1104/pp.96.2.485

Cited by 50 publications

(34 citation statements)

References 21 publications

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“…In maize, three Sus genes have been identified . Five Sus isoforms have been detected in Sorghum bicolor (Chourey et al, 1991). Silvente et al (2003) indicated that there are at least two Sus genes in common beans (P. vulgaris).…”

Section: Sucrose Synthase (Sus)mentioning

confidence: 99%

Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development

Abid

Silué

Muhovski

et al. 2009

Gene

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Received by A.J. van WijnenKeywords: myo-inositol phosphate synthase (MIPS) Sucrose synthase (Sus) Seed developmentThe aim of this review is to highlight the role of myo-inositol phosphate synthase (MIPS), which catalyses the first step in inositol biosynthesis and of sucrose synthase (Sus), an enzyme involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis. These two enzymes are involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses. The study of mutated MIPS and Sus genes in some crops, such as soybean and cotton, has shown that these two proteins are directly involved in embryogenesis. They exhibit several isoforms that are essential for normal seed development. The possible role of both genes in seed development is discussed in this review.

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Section: Sucrose Synthase (Sus)mentioning

confidence: 99%

Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development

Abid

Silué

Muhovski

et al. 2009

Gene

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“…The filter was probed with a 32 P-labeled potato sucrose synthase cDNA clone (Salanoubat and Belliard, 1987 Wang et al, 1993b) and was similar to that of the potato sucrose synthase transcript from tubers (Salanoubat and Belliard, 1987). Analysis of sucrose synthase copy number in maize (Echt and Chourey, 1985) and sorghum (Chourey et al, 1991b) indicates that there are two genes per haploid genome. Southern blot analysis of tomato genomic DNA hybridized under identical conditions as the northern blots indicated that the probe used in these studies hybridized to only one RNA species (data not shown).…”

Section: Sucrose Synthase Gene Expression In Tomatomentioning

confidence: 99%

“…Its main physiological importance is to cleave SUC, providing UDP-Glc for starch, cellulose, hemicellulose synthesis, and respiration (Huber and Akazawa 1986;Sung et al, 1988;Chourey et al, 1991b).…”

mentioning

confidence: 99%

Temporal and Spatial Expression Pattern of Sucrose Synthase during Tomato Fruit Development

Wang¹,

Smith²,

Brenner³

1994

Plant Physiol.

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Sucrose synthase is proposed to play an important role in the early stages of tomato fruit (Lycopersicon esculentum Mill.) growth. In this work, the temporal and spatial expression patterns of sucrose synthase during tomato fruit development were investigated. Fruit contained the majority of the sucrose synthase protein and mRNA relative to other organs. Only trace levels of sucrose synthase protein and mRNA were detected in the stem, petiole, and roots. Sucrose synthase mRNA was detected in pistils prior to anthesis, reached peak levels in fruit 5 to 7 d after anthesis (DAA), and was not detectable after 35 DAA. Sucrose synthase protein levels reached a maximum at 20 to 25 DAA and then declined to nondetectable levels after 45 DAA. The lack of coordination between protein and mRNA levels suggests that sucrose synthase expression may be controlled at the levels of both transcription and translation. Sucrose synthase mRNA was differentially localized in the fruit, being most abundant in the mesocarp cells adjacent to the placenta, the columella, and the cells surrounding the vascular bundle. Except around the vascular tissue, the localization of sucrose synthase mRNA positively correlates with starch granule accumulation at the cellular level.

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“…Excised embryos were homogenized using 1.0 g of fresh weight to 5.0 ml of grinding buffer (0.01 M Tris-maleate, pH 7.0), strained through glass wool, and centrifuged at 20,000 x g for 20 min (21). Samples were electrophoresed on a 4.5-7.0% linear gradient nondenaturing polyacrylamide gel, electroblotted to nitrocellulose, and immunostained using the appropriate primary antibody as detailed previously (16,21,22). DNA Analyses.…”

mentioning

confidence: 99%

Epistatic interaction and functional compensation between the two tissue- and cell-specific sucrose synthase genes in maize.

Chourey¹,

Taliercio²

1994

Proc. Natl. Acad. Sci. U.S.A.

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A tissue-specific epistatic mode of gene interaction was observed between molecularly homologous genes Sh1 and Sus1 (hereafter, Sh and Sus), encoding the sucrose synthase (SS) isozymes, SS1 and SS2, respectively. In Sh Sus genotype, both SS genes were expressed simultaneously and approximately equally in young seedlings; however, only the Sus-encoded SS2 protein was seen in the developing embryos. By contrast, the mutant sus genotype, lacking detectable levels of the SS2 protein in various tissues tested, showed expression of the Sh locus as judged by the detection of the SS1 protein in such embryos. Ectopic expression in embryos was seen from two separate Sh alleles, Sh-W22 and Sh'-5 (a revertant allele derived upon Ds excision from sh-m5933). In each case, the Sh expression at the protein level in embryos was unique to genotypes with the mutant sus gene. Based on the observed lack of phenotypic change in the sus mutant, we suggest that the ectopic expression of the Sh in otherwise Sus-specific tissues leads to functional compensation. There was no epistatic interaction of Sh and Sus at the RNA level as SS1 transcripts were detectable in both Sus and sus embryos. Thus, embryo specificity between the two SS genes was determined at posttranscriptional or at translational level of control. We surmise on the basis of these data that metabolic regulatory controls seem to override the normal constraints of tissue and cell specificity of the nonallelic isozyme genes to maintain efficient use of the pathways.

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Tissue-Specific Expression and Anaerobically Induced Posttranscriptional Modulation of Sucrose Synthase Genes in Sorghum bicolor M.

Cited by 50 publications

References 21 publications

Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development

Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development

Temporal and Spatial Expression Pattern of Sucrose Synthase during Tomato Fruit Development

Epistatic interaction and functional compensation between the two tissue- and cell-specific sucrose synthase genes in maize.

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