Tissue-specific expression, induction, and inhibition through metabolic intermediate-complex formation of guinea pig cytochrome P450 belonging to the CYP2B subfamily

Yamada, Hideyuki; Kaneko, Hayato; Takeuchi, Kenji; Oguri, Kazuta; Yoshimura, Hidetoshi

doi:10.1016/0003-9861(92)90271-w

Cited by 10 publications

(11 citation statements)

References 39 publications

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“…To clarify the mechanism whereby PB increases CYP2B expression, it would be useful to focus on such differences and mutant animal models. Thus, we examined the genomic sequences of the guinea pig, a species less responsive to PB [15,16], and the Qdj:SD rat, a mutant strain lacking inducible expression of the CYP2B2 gene [174]. The results obtained show that there is no PBREM 5'-upstream of the guinea pig CYP2B18 gene [175], which agrees with the importance of PBREM in obtaining a PB-response.…”

Section: Xenobiotic Regulation Of Cyp2b Expre-ssionsupporting

confidence: 80%

“…Furthermore, the ability to metabolize strychnine differs from species to species. Such a difference in strychnine metabolism is correlated with the expression level of CYP2B isozymes in each species [14][15][16]. These observations suggest that animals expressing highly-inducible CYP2Bs are more tolerant of the toxic effects of strychnine than other species with poorly-inducible enzymes.…”

Section: Induction Of Cyp2b: Its Evolutional Significancementioning

confidence: 83%

“…It is known that there are marked species differences in CYP2B induction: for example, while rats exhibit marked induction following PB treatment, guinea pigs express a constitutively high level of CYP2B18 and exhibit only a low induction with PB [15,16]. To clarify the mechanism whereby PB increases CYP2B expression, it would be useful to focus on such differences and mutant animal models.…”

Section: Xenobiotic Regulation Of Cyp2b Expre-ssionmentioning

confidence: 98%

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Induction of the Hepatic Cytochrome P450 2B Subfamily by Xenobiotics: Research History, Evolutionary Aspect, Relation to Tumorigenesis, and Mechanism

Yamada

Ishii²,

Yamamoto

et al. 2006

CDM

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The cytochrome P450 belonging to the CYP2B subfamily has long been of great interest because it can be induced by xenobiotics. While a well known diagnostic ligand-receptor theory explains the induction of the CYP1A subfamily, the mechanism by which xenobiotics induce the CYP2B subfamily is not fully understood. Although the constitutive androstane receptor (CAR) undoubtedly plays a crucial role in the induction, many questions regarding the mechanism of CAR activation by xenobiotics have not yet been answered. It is a puzzle that many structurally-unrelated chemicals can increase the expression of the CYP2B subfamily. This may support a mechanism(s) distinct from the signaling induced by ligand-receptor binding. Indeed, phenobarbital, a typical inducer, cannot associate with CAR. Thus, no one is able to answer a fundamental question: what is the initial target of xenobiotics to produce induced expression of CYP2B enzymes? In this review, we survey the research history of CYP2B induction, list the inducers reported so far, and discuss the mechanism of induction including the target site of inducers.

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Section: Xenobiotic Regulation Of Cyp2b Expre-ssionsupporting

confidence: 80%

Section: Induction Of Cyp2b: Its Evolutional Significancementioning

confidence: 83%

Section: Xenobiotic Regulation Of Cyp2b Expre-ssionmentioning

confidence: 98%

See 1 more Smart Citation

Induction of the Hepatic Cytochrome P450 2B Subfamily by Xenobiotics: Research History, Evolutionary Aspect, Relation to Tumorigenesis, and Mechanism

Yamada

Ishii²,

Yamamoto

et al. 2006

CDM

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“…Many of the above named drugs are subject to cytochrome P450‐mediated drug oxidation ( Jurima‐Romet et al ., 1993 , Stearns et al ., 1995 , Oldham et al ., 1997 , Tracy et al ., 1999 ), but surprisingly, little is known about the metabolic capacity of the heart. Indeed, there is very limited information on the expression of P450 isozymes in heart tissue of laboratory animals ( Stegeman et al ., 1982 , Geetha et al ., 1991 , Yamada et al ., 1992 , McCallum et al ., 1993 , Fulton et al ., 1995 , Xiao et al ., 1998 ) despite their importance in the metabolism of drugs.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P450 mono‐oxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat

Thum

Borlak

2000

British J Pharmacology

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1 There are a substantial number of drugs acting either directly or indirectly on the heart, but surprisingly, little is known about the metabolic capacity of heart muscle cells. 2 We therefore investigated the gene expression and protein activity of cytochrome P450 isozymes in cultures of adult cardiomyocytes of the rat. 3 Semi-quantitative CYP gene expression pattern suggests CYP1A1 and CYP2B1/2 to be key players in cardiomyocytes and upon treatment with Aroclor 1254 approximate 4 fold inductions could be observed for both gene families, when compared with appropriate controls. 4 The mRNA expression of most genes was sustained for prolonged periods of time, e.g. up to 120 h in culture and in the case of the CYP3A1 gene an approximate 10 fold induction was observed at the higher Aroclor 1254 dose level (10 mM) in 24 h old cultures. 5 The constitutively expressed genes, e.g. CYP2C11 and CYP2E1 are expressed throughout the entire culture period (5 days) and did not respond to Aroclor 1254 treatment. 6 CYP4A1 was mainly expressed in freshly isolated cardiomyocytes of control animals and its expression declined rapidly in culture. 7 There was good agreement between gene expression and translated protein activity using 7-ethoxyresoru®n and testosterone as substrates. 8 The data reported herein should foster the routine use of freshly isolated and cultivated cardiomyocytes for drug pro®ling and toxicity studies.

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“…Interestingly, coadministration of a-tocopherol reduced signi® cantly the alteration observed in microsomes of rat heart tissue. The tissue-speci® c expression, induction and inhibition through metabolic intermediate complex formation of guinea pig P450 was studied by Yamada et al (1992). They reported that treatment with dexamethasone or p,p« -dichlorodiphenyltrichloroethane and}or isoafrole produced very signi® cant induction in the expression of P450 isozymes in guinea pig heart tissue.…”

Section: Cytochrome P450 (P450) System In Cardiomyocytesmentioning

confidence: 99%

Isolation and cultivation of Ca²⁺tolerant cardiomyocytes from the adult rat: improvements and applications

Thum

Borlak

2000

Xenobiotica

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1. Primary cultures of cardiomyocytes provide a valuable tool for the study of the pharmacological and toxicological properties of drugs and chemicals, but for several technical reasons cardiomyocytes from adult animals are not routinely used in long-term culture. Because of significant advances in cardiovascular research, tissue engineering and cell transplantation, the need to isolate primary cells from adult animal and/or human tissue is likely to increase in the future. 2. The most common protocols for the isolation and cultivation of cardiomyocytes have been reviewed and the various approaches have been compared. The recent advances in cell culture techniques and the use of the cytoprotective agent, e.g. 2,3-butanedione monoxime greatly increases cell yield and cell viability of isolated and cultured cardiomyocytes. New concepts emerge that enabled an assessment of cellular differentiation in cultured cardiomyocytes and certain specific nuclear transcription factors may play a pivotal role in this process.

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Tissue-specific expression, induction, and inhibition through metabolic intermediate-complex formation of guinea pig cytochrome P450 belonging to the CYP2B subfamily

Cited by 10 publications

References 39 publications

Induction of the Hepatic Cytochrome P450 2B Subfamily by Xenobiotics: Research History, Evolutionary Aspect, Relation to Tumorigenesis, and Mechanism

Induction of the Hepatic Cytochrome P450 2B Subfamily by Xenobiotics: Research History, Evolutionary Aspect, Relation to Tumorigenesis, and Mechanism

Cytochrome P450 mono‐oxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat

Isolation and cultivation of Ca²⁺tolerant cardiomyocytes from the adult rat: improvements and applications

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Tissue-specific expression, induction, and inhibition through metabolic intermediate-complex formation of guinea pig cytochrome P450 belonging to the CYP2B subfamily

Cited by 10 publications

References 39 publications

Induction of the Hepatic Cytochrome P450 2B Subfamily by Xenobiotics: Research History, Evolutionary Aspect, Relation to Tumorigenesis, and Mechanism

Induction of the Hepatic Cytochrome P450 2B Subfamily by Xenobiotics: Research History, Evolutionary Aspect, Relation to Tumorigenesis, and Mechanism

Cytochrome P450 mono‐oxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat

Isolation and cultivation of Ca2+tolerant cardiomyocytes from the adult rat: improvements and applications

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Product

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Isolation and cultivation of Ca²⁺tolerant cardiomyocytes from the adult rat: improvements and applications