Tissue specific expression of SG2NA is regulated by differential splicing, RNA editing and differential polyadenylation

Jain, Buddhi Prakash; Chauhan, Pooja; Tanti, Goutam Kumar; Singarapu, Nandini; Ghaskadbi, Surendra; Goswami, Shyamal

doi:10.1016/j.gene.2014.11.045

Cited by 18 publications

(14 citation statements)

References 20 publications

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“…Additionally, variations in amino acid as a result of RNA editing occurred most frequently in serine to leucine which reflects a conversion into a hydrophobic amino acid from a hydrophilic, while arginine to cysteine occurred less frequently (Table S2). These general features of advanced RNA editing in the plants cp genomes have also been documented by previous studies [55,56] which found that important RNA editing sites direct amino acid to alter from polar to non-polar in order to form a fundamental organization of protein to enhance protein hydrophobicity [57].…”

Section: Rna Editing Sitessupporting

confidence: 67%

Complete Chloroplast Genomes of Chlorophytum comosum and Chlorophytum gallabatense: Genome Structures, Comparative and Phylogenetic Analysis

Munyao

Dong

Yang

et al. 2020

Plants

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The genus Chlorophytum includes many economically important species well-known for medicinal, ornamental, and horticultural values. However, to date, few molecular genomic resources have been reported for this genus. Therefore, there is limited knowledge of phylogenetic studies, and the available chloroplast (cp) genome of Chlorophytum (C. rhizopendulum) does not provide enough information on this genus. In this study, we present genomic resources for C. comosum and C. gallabatense, which had lengths of 154,248 and 154,154 base pairs (bp), respectively. They had a pair of inverted repeats (IRa and IRb) of 26,114 and 26,254 bp each in size, separating the large single-copy (LSC) region of 84,004 and 83,686 bp from the small single-copy (SSC) region of 18,016 and 17,960 bp in C. comosum and C. gallabatense, respectively. There were 112 distinct genes in each cp genome, which were comprised of 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. The comparative analysis with five other selected species displayed a generally high level of sequence resemblance in structural organization, gene content, and arrangement. Additionally, the phylogenetic analysis confirmed the previous phylogeny and produced a phylogenetic tree with similar topology. It showed that the Chlorophytum species (C. comosum, C. gallabatense and C. rhizopendulum) were clustered together in the same clade with a closer relationship than other plants to the Anthericum ramosum. This research, therefore, presents valuable records for further molecular evolutionary and phylogenetic studies which help to fill the gap in genomic resources and resolve the taxonomic complexes of the genus.

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Section: Rna Editing Sitessupporting

confidence: 67%

Complete Chloroplast Genomes of Chlorophytum comosum and Chlorophytum gallabatense: Genome Structures, Comparative and Phylogenetic Analysis

Munyao

Dong

Yang

et al. 2020

Plants

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“…1A ). However, it is the most abundant but not exclusive isoform in different tissues 11 . Owing to the prevalence of only one isoform, we selected NIH3T3 cells as a model for studying the role of SG2NA in cell physiology.…”

Section: Resultsmentioning

confidence: 99%

“…Subsequent studies suggested that it is a n uclear protein/ a ntigen with increased expression during S and G2 phases of cell cycle and named accordingly 9 . It has at least six isoforms generated by alternative splicing and RNA editing 10 , 11 . These variants are differentially expressed in mouse tissues and cultured cells 11 .…”

Section: Introductionmentioning

confidence: 99%

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GSK3β and ERK regulate the expression of 78 kDa SG2NA and ectopic modulation of its level affects phases of cell cycle

Pandey

Talukdar

Jain

et al. 2017

Sci Rep

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Striatin and SG2NA are essential constituents of the multi-protein STRIPAK assembly harbouring protein phosphatase PP2A and several kinases. SG2NA has several isoforms generated by mRNA splicing and editing. While the expression of striatin is largely restricted to the striatum in brain, that of SG2NAs is ubiquitous. In NIH3T3 cells, only the 78 kDa isoform is expressed. When cells enter into the S phase, the level of SG2NA increases; reaches maximum at the G2/M phase and declines thereafter. Downregulation of SG2NA extends G1 phase and its overexpression extends G2. Ectopic expression of the 35 kDa has no effects on the cell cycle. Relative abundance of phospho-SG2NA is high in the microsome and cytosol and the nucleus but low in the mitochondria. Okadoic acid, an inhibitor of PP2A, increases the level of SG2NA which is further enhanced upon inhibition of proteasomal activity. Phospho-SG2NA is thus more stable than the dephosphorylated form. Inhibition of GSK3β by LiCl reduces its level, but the inhibition of ERK by PD98059 increases it. Thus, ERK decreases the level of phospho-SG2NA by inhibiting GSK3β. In cells depleted from SG2NA by shRNA, the levels of pGSK3β and pERK are reduced, suggesting that these kinases and SG2NA regulate each other’s expression.

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“…We have reported earlier that SG2NA has several isoforms generated out of alternative splicing [7]. Expression profile of SG2NAs in tissues changes with stages of embryonic development and post-natal aging [7,8,9]. Expression of SG2NA is epigenetically regulated by Brg-1 [10].…”

Section: Introductionmentioning

confidence: 99%

SG2NA enhances cancer cell survival by stabilizing DJ-1 and thus activating Akt

Tanti

Pandey

Goswami

2015

Biochemical and Biophysical Research Communications

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Tissue specific expression of SG2NA is regulated by differential splicing, RNA editing and differential polyadenylation

Cited by 18 publications

References 20 publications

Complete Chloroplast Genomes of Chlorophytum comosum and Chlorophytum gallabatense: Genome Structures, Comparative and Phylogenetic Analysis

Complete Chloroplast Genomes of Chlorophytum comosum and Chlorophytum gallabatense: Genome Structures, Comparative and Phylogenetic Analysis

GSK3β and ERK regulate the expression of 78 kDa SG2NA and ectopic modulation of its level affects phases of cell cycle

SG2NA enhances cancer cell survival by stabilizing DJ-1 and thus activating Akt

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