Tissue specific expression profile of some immune related genes in Labeo rohita to Edwardsiella tarda infection

Kole, Sajal; Anand, Deepika; Sharma, Rama; Tripathi, Gayatri; Makesh, M.; Rajendran, K. V.; Bedekar, Megha Kadam

doi:10.1016/j.fsi.2017.05.047

Cited by 22 publications

(4 citation statements)

References 29 publications

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“…In the present study, the observed increase in il-1β expression from infected gilthead seabream was in line with that already observed in teleosts submitted to bacterial diseases. In fact, il-1β expression evaluation after a bacterial challenge is a common approach, and similar studies have been performed [24,[43][44][45] resulting in analogous variations. Indeed, IL-1β is a pro-inflammatory cytokine with a key role in the first stages of inflammation by attracting fish leucocytes [46].…”

Section: Discussionmentioning

confidence: 99%

Short-Term Immune Responses of Gilthead Seabream (Sparus aurata) Juveniles against Photobacterium damselae subsp. piscicida

Santos

Peixoto

Ferreira

et al. 2022

IJMS

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Photobacteriosis is a septicaemic bacterial disease affecting several marine species around the globe, resulting in significant economic losses. Although many studies have been performed related to the pathogen virulence and resistance factors, information regarding the host defence mechanisms activated once an infection takes place is still scarce. The present study was designed to understand innate immune responses of farmed juvenile gilthead seabream (Sparus aurata) after Photobacterium damselae subsp. piscicida (Phdp) infection. Therefore, two groups of seabream juveniles were intraperitoneally injected with 100 µL of PBS (placebo) or 100 µL of exponentially growing Phdp (1 × 106 CFU/mL; infected). The blood, plasma, liver, and head kidney of six fish from each treatment were sampled immediately before infection and 3, 6, 9, 24 and 48 h after infection for the broad screening of fish immune and oxidative stress responses. Infected animals presented marked anaemia, neutrophilia and monocytosis, conditions that are correlated with an increased expression of genes related to inflammation and phagocytic activity. Similar studies with different fish species and bacteria can be useful for the definition of health biomarkers that might help fish farmers to prevent the occurrence of such diseases.

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Section: Discussionmentioning

confidence: 99%

Short-Term Immune Responses of Gilthead Seabream (Sparus aurata) Juveniles against Photobacterium damselae subsp. piscicida

Santos

Peixoto

Ferreira

et al. 2022

IJMS

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“…In another study, common carp fed with guava leaf powder enriched diets showed significant up-regulation of IL-1β while there was no change in the expression of TNF-α [7]. The expressions of NOD 1 and TLR-22 were higher in liver compared to kidney [48]. Tissue-specific expressions of various genes were reported in catla [42].…”

Section: Discussionmentioning

confidence: 94%

Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions

Kumar

Sharma

Singh

et al. 2019

Heliyon

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The immunostimulatory and disease resistance properties of herb Achyranthes aspera L. (Amaranthaceae) were evaluated in rohu Labeo rohita in pond. Rohu fry (1.9 ± 0.08 g) were cultured in hapas (25 hapa −1 ) set inside a pond and were fed with two experimental diets containing 0.5% seeds (D1) and leaves (D2) of A. aspera and control diet (D3). Fish were challenged with Aeromonas hydrophila after 80 days. The cumulative mortality rate of fish was significantly ( P < 0.05) higher in D3 (28–48%) compared to others. Average weight was significantly ( P < 0.05) higher in D1 (6.5–12.5%) compared to other treatments. Myeloperoxidase and nitric oxide synthase levels were significantly ( P < 0.05) higher in D1 and D2 compared to D3. Thiobarbituric acid reactive substances and carbonyl protein levels were significantly ( P < 0.05) lower in hepatopancreas and kidney of D1 compared to others. In hepatopancreas, the expressions of lysozyme C, loysozyme G, TNF-α, IL-10 and IL-1β were significantly ( P < 0.05) higher in D1 compared to others. This treatment was followed by D2. In kidney, lysozyme G and TNF-α levels were significantly ( P < 0.05) higher in D1 and D2 compared to D3. Whereas, IL-10 and IL-1β were significantly ( P < 0.05) down-regulated and up-regulated, respectively in kidney of D2. There was up-regulation ( P < 0.05) of TLR-4 in hepatopancreas and kidney of D1 and D2 diets fed rohu, respectively compared to others.

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“…Kole and colleagues analysed the tissue‐specific expression profiles of the immune‐related genes (i.e. IgHC, NOD 1, TLR 22, iNOS and IL‐1β ) in L. rohita during experimental challenge infections with Edwardsiella tarda to examine their functions in pathogenesis using qRT‐PCR (Kole et al, 2017). TLR22 and IgHC were significantly expressed upon infection, depicting their defensive roles against bacterial infection.…”

Section: Molecular and Genomics Techniquesmentioning

confidence: 99%

Status of genetic and genomic approaches for delineating biological information and improving aquaculture production of farmed rohu, Labeo rohita (Ham, 1822)

Rasal

Sundaray

2020

Reviews in Aquaculture

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Farmed rohu, Labeo rohita, is an economically important freshwater aquaculture species in south Asia. Consumer preference for this species has driven researchers to develop genetically improved rohu (Jayanti) for important traits such as growth and disease resistance. The feeding and breeding biology of rohu is well‐studied, but the physiological responses to stress or climate change at the molecular level are less understood. We summarized the genetic and advanced molecular tools used for biological insights into farmed rohu. Recently, next‐generation sequencing technology (NGS) and genome informatics have helped to identify biological questions in fish using genome, transcriptome, metagenome, proteome and epigenome sequencing. We briefly reviewed past rohu research using traditional selective breeding methods for genetic improvement, as well as the other molecular tools that have been utilized to understand the fish’s physiology, health, nutrition, stress and reproduction. We discuss the molecular techniques used in rohu, such as gene cloning and expression profiling, DNA markers, NGS, metagenomics, epigenomics, proteomics, cell culture/cell line development and transgenics. Finally, we address the future perspectives of rohu research, which may be driven by NGS and other advanced techniques like genome editing to improve rohu aquaculture production. The comprehensive information presented here will provide insights for the carp aquaculture research community.

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Tissue specific expression profile of some immune related genes in Labeo rohita to Edwardsiella tarda infection

Cited by 22 publications

References 29 publications

Short-Term Immune Responses of Gilthead Seabream (Sparus aurata) Juveniles against Photobacterium damselae subsp. piscicida

Short-Term Immune Responses of Gilthead Seabream (Sparus aurata) Juveniles against Photobacterium damselae subsp. piscicida

Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions

Status of genetic and genomic approaches for delineating biological information and improving aquaculture production of farmed rohu, Labeo rohita (Ham, 1822)

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