Tissue-specific metabolite profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS

Zhao, Yujiao; Chu, Shanshan; Gui, Shuangying; Qin, Yuejian; Xu, Rui; Shan, Tingyu; Peng, Hua-Sheng

doi:10.1016/j.jpba.2021.114070

Cited by 22 publications

(12 citation statements)

References 19 publications

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“…Most medicinally active compounds are plant secondary metabolites and primarily accumulate in tissues or cells [25]. Laser microdissection (LMD) has been used to separate specific tissue or single cells of medicinal plants under a microscope [26][27][28][29][30]. Recent studies have reported that the volatile oils of A. lancea are mainly present in the rhizome.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis in different organs and tissue‐specific metabolite profiling of Atractylodes lancea from four regions by GC–MS and laser microdissection

et al. 2022

J of Separation Science

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Traditional Chinese medicine is made from the rhizome of Atractylodes lancea (Thunb.) DC. (Compositae), known as Cangzhu. In this study, gas chromatography‐mass spectrometry was used to identify and quantify the volatile oils of different organs of A. lancea from four regions of China: Jiangsu, Anhui, Henan, and Hubei provinces. The volatile oils of A. lancea were qualitatively and quantitatively characterized using gas chromatography‐mass spectrometry combined with laser microdissection. The results identified 21 components in A. lancea, the majority of the components were found in the rhizomes, followed by the fibrous roots, flowers, leaves, and stems. According to the contents of volatile oils in A. lancea, it was divided into Dabieshan (mainly includes hinesol and β‐eudesmol) and Maoshan types (mainly includes atractylon and atractylodin), and the ratios of hinesol:β‐eudesmol:atractylon:atractylodin were 17.06:4.55:0:1, 12.66:11.71:0.99:1, 7.43:6.23:0:1, and 0.13:0.16:1.52:1 in A. lancea from AH, HN, HB, and JS, respectively. Tissue‐specific study indicated that Dabieshan type mainly includes elemol, hinesol, and β‐eudesmol in the periderm and secretory cavities of A. lancea, whereas Maoshan type mainly includes atractylon, atractylodin, little hinesol, and β‐eudesmol in the secretory cavities. Conversely, no volatile oils were detected in the cortex, phloem, xylem, vascular ray, or pith. This study provides a foundation for further evaluation and utilization of A. lancea.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis in different organs and tissue‐specific metabolite profiling of Atractylodes lancea from four regions by GC–MS and laser microdissection

et al. 2022

J of Separation Science

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“…However, overuse of pesticides increases planting costs, causing the harmful substances in P. lactiflora roots to exceed the standards, which in turn affects the quality of Shaoyao. Previous studies have found that different morphological characters can affect the quality of medicinal materials (Chen et al, 2014;Chu et al, 2020;Zhao et al, 2021). The "pockmarks" is a special morphological character of Shaoyao, how it impacts on the quality of Shaoyao needs further investigation.…”

Section: Discussionmentioning

confidence: 98%

Comparative Elucidation of Age, Diameter, and “Pockmarks” in Roots of Paeonia lactiflora Pall. (Shaoyao) by Qualitative and Quantitative Methods

et al. 2022

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Paeonia lactiflora Pall. is a world-famous ornamental plant, whose roots have been used as an important traditional Chinese medicine, Shaoyao, to treat diseases for more than 1,000 years. Because of the excellent curative effect of Shaoyao, its quality has attracted wide attention, however, there is a lack of comprehensive research on the different influencing factors of quality of Shaoyao. In this study, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and high-performance liquid chromatography with diode-array detection (HPLC-DAD) were utilized to systematically analyze the Shaoyao of different ages, diameters and roots with “pockmarks.” 60 metabolites were detected and identified from Shaoyao using the UPLC-Q/TOF-MS, of which 20 potential quality markers of dissected roots with and without “pockmarks” were selected for the first time using the orthogonal partial least squares discriminant analysis (OPLS-DA) and the variable importance for projection (VIP) plot. Then, a selective and accurate HPLC-DAD quantitative assay has been developed for the simultaneous determination of 11 bioactive components in Shaoyao. The results showed that the total content of five monoterpene glycosides including oxypaeoniflorin, albiflorin, paeoniflorin, lactiflorin, and benzoylpaeoniflorin and six phenols including gallic acid, catechin, methyl gallate, ethyl gallate, apiopaeonoside and benzoic acid in the 3-year-old Shaoyao was higher than that of 4-year-old and 5-year-old Shaoyao. In Shaoyao of the same age, the total content of five monoterpene glycosides and six phenols decreased with an increase in diameter. In addition, regardless of whether it is a whole or a divided root, the contents of five monoterpene glycosides and six phenols in Shaoyao with “pockmarks” were higher than those of Shaoyao without “pockmarks.” In summary, this work has explored several factors that might affect the quality of Shaoyao, and provide a guide for more comprehensive quality evaluation in its further production, processing, and rational utilization.

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“… Glycyrrhiza glabra Rhizome Flavonoids, Saponins MALDI [ 105 ] Paeonia lactiflora Root Gallotannins MALDI [ 111 ] Scutellaria baicalensis Root Flavonoids PALDI [ 135 ] Tripterygium wilfordii Root Triterpinoids MALDI [ 104 ] Fallopia multiflora Fallopia multiflora var. angulata Root Stilbene Glucosides, Anthraquinones, Flavonoids MALDI [ 136 ] Curcuma longa Root Polyphenols MALDI [ 106 ] Panax ginseng Panax quinquefolius Panax notoginseng Root Triterpenoid Saponins MALDI [ 137 ] Podophyllum hexandrum Root Alkaloids MALDI [ 138 ] Aquilaria sinensis Stem Chromones MALDI [ 26 ] Mentha piperita Leaf Flavonoids ...…”

Section: Main Textmentioning

confidence: 99%

“…Additionally, the untargeted analysis function of MSI allowed the detection of other endogenous metabolites such as quercetin, emodin and physcion. Spatial information of representative natural products might be beneficial for the quality evaluation of prepared slices of Polygoni Multiflori Radix [ 136 ].…”

Section: Main Textmentioning

confidence: 99%

The application of mass spectrometry imaging in traditional Chinese medicine: a review

et al. 2022

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Mass spectrometry imaging is a frontier technique which connects classical mass spectrometry with ion imaging. Various types of chemicals could be visualized in their native tissues using mass spectrometry imaging. Up to now, the most commonly applied mass spectrometry imaging techniques are matrix assisted laser desorption ionization mass spectrometry imaging, desorption electrospray ionization mass spectrometry imaging and secondary ion mass spectrometry imaging. This review gives an introduction to the principles, development and applications of commonly applied mass spectrometry imaging techniques, and then illustrates the application of mass spectrometry imaging in the investigation of traditional Chinese medicine. Recently, mass spectrometry imaging has been adopted to explore the spatial distribution of endogenous metabolites in traditional Chinese medicine. Data collected from mass spectrometry imaging can be further utilized to search for marker components of traditional Chinese medicine, discover new compounds from traditional herbs, and differentiate between medicinal plants that are similar in botanical features. Moreover, mass spectrometry imaging also plays a role in revealing the pharmacological and toxicological mechanisms of traditional Chinese medicine.

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Tissue-specific metabolite profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS

Cited by 22 publications

References 19 publications

Comparative analysis in different organs and tissue‐specific metabolite profiling of Atractylodes lancea from four regions by GC–MS and laser microdissection

Comparative analysis in different organs and tissue‐specific metabolite profiling of Atractylodes lancea from four regions by GC–MS and laser microdissection

Comparative Elucidation of Age, Diameter, and “Pockmarks” in Roots of Paeonia lactiflora Pall. (Shaoyao) by Qualitative and Quantitative Methods

The application of mass spectrometry imaging in traditional Chinese medicine: a review

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