Titanium and zirconia particle-induced pro-inflammatory gene expression in cultured macrophages and osteolysis, inflammatory hyperalgesia and edema in vivo

Obando-Pereda, Gustavo Alberto; Fischer, Luana; Stach-Machado, Dagmar Ruth

doi:10.1016/j.lfs.2013.11.008

Cited by 61 publications

(52 citation statements)

References 42 publications

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“…For example, TNF-α was expressed at 12 h for N, LM and LN groups and the protein production did not occur at 12 h or even after 24 h. Another finding was that IL-6 had the highest-fold increase and IL1-β produced more proteins. Other studies in the orthopedic literature described similar results to those of present study (11,15,22), but the reasons for this are unknown. A possible explanation may be that this discrepancy in mRNA expression and protein production is related to post-transcriptional processing or autocrine mechanisms to prevent an over-inflammatory reaction that can be harmful for the system (15).…”

Section: Pro-inflammatory Analysis Of Macrophagessupporting

confidence: 85%

“…The cells were differentiated to macrophages using 125 ng/well for 48 h of phorbol 12 -myristate 13-acetate (PMA) (Sigma Aldrich, St. Louis, MO, USA) (14). The wells were washed 3 times with PBS and treated according to the following 6 experimental groups: no treatment, Control (C); 1 μg/mL lipopolysaccharide from P. gingivalis (L) (15); 50 ng/mL of titanium microparticles (M); 50 ng/mL titanium nanoparticles (N); 1 μg/mL of lipopolysaccharide from P. gingivalis plus 50 ng/mL microparticles (LM); 1 μg/mL of lipopolysaccharide from P. gingivalis plus 50 ng/mL of titanium nanoparticles (LN). The treatments were diluted in RPMI-1640 medium and 5 mL was placed in each well of a 6-well plate (12).…”

Section: Cell Culturementioning

confidence: 99%

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Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

et al. 2017

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During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey's test was used (p<0.05). After treatment, cells presented similar viability and morphology demonstrating that the treatments were not able to induce cell death. Gene expression was significantly higher for TNF-α and IL1-β after 12 h, and for IL-6 after 24 h in the N and LN groups. Cytokine production over time was an ascending curve for TNF-α with the peak at 48 h and IL1-β and IL-6 had a straight line among the time points, although cells from N group presented a significant production of IL-6 at 48 h. In conclusion, these results suggest that titanium nanoparticles stimulate stronger pro-inflammatory response in macrophages, independent of their association with LPS from P.gingivalis.

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Section: Pro-inflammatory Analysis Of Macrophagessupporting

confidence: 85%

Section: Cell Culturementioning

confidence: 99%

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

et al. 2017

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“…Titanium and metal particle release is discussed as cause for implant failure as well as implant dentistry [5, 6, 9, 56, 57]. It is assumed that metal particles influence the macrophage or lymphocyte pathways and provoke a release of pro-inflammatory cytokines, leading to an increased osteoclastogenesis and decreased osteoblastogenesis and consequently to peri-implant bone loss [58]. This osteolytic effect could be enhanced by vitamin D deficiency, since Maier et al demonstrated in an epidemiological study that vitamin D deficiency is associated with a higher risk to develop aseptic loosening around joint replacements caused by wear particles [59].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D deficiency in early implant failure: two case reports

et al. 2016

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An association between vitamin D deficiency and early dental implant failure is not properly verified, but its role in osteoimmunology is discussed. This article illustrates two case reports with vitamin D deficiency and early implant failure. Prior to implant placement, the first patient received crestal bone grafting with autologous material. Both patients received dental implants from different manufacturers in the molar region of the mandible. In the case of bone grafting in the first patient, all implants were placed in a two-stage procedure. All implants had to be removed within 15 days after implant placement. Vitamin D serum levels were measured: Both patients showed a vitamin D deficiency (serum vitamin D level <20 μg/l). After vitamin D supplementation, implant placement was successful in both patients. Prospective, randomized clinical trials must follow to affirm the relationship between vitamin D deficiency, osteoimmunology, and early implant failure.

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“…Although the mechanisms behind metal ion-induced bone regeneration are not well understood (reviewed in [43]), the presence of ions probably affects macrophages. Zirconia (in baghdadite) has been shown to be pro-inflammatory [44], whereas strontium and zinc (in Sr-HT-gahnite) have been shown to affect bone remodelling [45,46] and promote anti-inflammatory activity [47]. Future studies are focused on determining whether the presence of these ions contributed to the differential activation of macrophages observed in this work.…”

Section: Discussionmentioning

confidence: 83%

In vitroresponse of macrophages to ceramic scaffolds used for bone regeneration

Graney

Roohani‐Esfahani

Zreiqat

et al. 2016

J. R. Soc. Interface.

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Macrophages, the primary cells of the inflammatory response, are major regulators of healing, and mediate both bone fracture healing and the inflammatory response to implanted biomaterials. However, their phenotypic contributions to biomaterial-mediated bone repair are incompletely understood. Therefore, we used gene expression and protein secretion analysis to investigate the interactions in vitro between primary human monocytederived macrophages and ceramic scaffolds that have been shown to have varying degrees of success in promoting bone regeneration in vivo. Specifically, baghdadite (Ca 3 ZrSi 2 O 9 ) and strontium-hardystonite-gahnite (Sr-Ca 2 ZnSi 2 O 7 -ZnAl 2 O 4 ) scaffolds were chosen as two materials that enhanced bone regeneration in vivo in large defects under load compared with clinically used tricalcium phosphate-hydroxyapatite (TCP-HA). Principal component analysis revealed that the scaffolds differentially regulated macrophage phenotype. Temporal changes in gene expression included shifts in markers of pro-inflammatory M1, anti-inflammatory M2a and pro-remodelling M2c macrophage phenotypes. Of note, TCP-HA scaffolds promoted upregulation of many M1-related genes and downregulation of many M2a-and M2c-related genes. Effects of the scaffolds on macrophages were attributed primarily to direct cell-scaffold interactions because of only minor changes observed in transwell culture. Ultimately, elucidating macrophage-biomaterial interactions will facilitate the design of immunomodulatory biomaterials for bone repair.

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Titanium and zirconia particle-induced pro-inflammatory gene expression in cultured macrophages and osteolysis, inflammatory hyperalgesia and edema in vivo

Cited by 61 publications

References 42 publications

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Vitamin D deficiency in early implant failure: two case reports

In vitroresponse of macrophages to ceramic scaffolds used for bone regeneration

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