Titanium Culture Vessel Presenting Temperature Gradation for the Thermotolerance Estimation of Cells

Abstract

Hyperthermia can be induced to exploit the thermal intolerance of cancer cells, which is worse than that of normal cells, as a potential noninvasive cancer treatment. To develop an effective hyperthermia treatment, thermal cytotoxicity of cells should be comprehensively investigated. However, to conduct such investigations, the culture temperature must be accurately regulated. We previously reported a culture system in which the culture temperature could be accurately regulated by employing metallic culture vessels. However, appropriate temperature conditions for hyperthermia depend on the cell species. Consequently, several experiments need to be conducted, which is a bottleneck of inducing hyperthermia. Hence, we developed a cell culture system with temperature gradation on a metallic culture surface. Michigan Cancer Foundation-7 cells and normal human dermal fibroblasts were used as cancer and normal cell models, respectively. Normal cells showed stronger thermal tolerance; this was because the novel system immediately exhibited a temperature gradation. Thus, the developed culture system can be used to investigate the optimum thermal conditions for effective hyperthermia treatment. Furthermore, as the reactions of cultured cells can be effectively assessed with the present results, further research involving the thermal stimulation of cells is possible.

Clemastine and hyperthermia enhance sensitization of osteosarcoma cells for apoptosis

“…Various reports suggest that hyperthermia-mediated treatment in combination with chemotherapy is effective on various cancers. In one study, 9 breast cancer cells (MCF-7) were monitored by a metallic culture surface that could evaluate cellular reactions over a range of temperature stimuli, which makes it easier for the hyperthermia treatment to be accurately controlled. This would provide a suitable temperature for an effective hyperthermia treatment.…”

“…This would provide a suitable temperature for an effective hyperthermia treatment. 9 Maduabuchi et al 10 analyzed the effect of hyperthermia at 43°C or 47°C for 1 h on pancreatic adenocarcinoma cells. The study indicated that hyperthermic treatment (under defined environmental conditions) affects pancreatic adenocarcinoma cell-to-cell contact and oxygen status and increases endothelial sprouting by HIF-1α and VEGF secretion which play a role in the inhibition of pancreatic adenocarcinoma.…”