Titanium dioxide nanoparticles induce strong oxidative stress and mitochondrial damage in glial cells

Huerta-García, Elizabeth; Pérez-Arizti, José Antonio; Márquez-Ramírez, Sandra Gissela; Delgado-Buenrostro, Norma L.; Chirino, Yolanda I.; Iglesias, Gisela Gutiérrez; López‐Marure, Rebeca

doi:10.1016/j.freeradbiomed.2014.04.026

Cited by 168 publications

(99 citation statements)

References 53 publications

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“…Group lead by Huerta-García showed that TiO2 NPs do not cause mitochondrial depolarisation after 24 h of exposure but Ag NPs caused significant mitochondrial depolarisation which indicated cytotoxicity. However, they also suggested that cytochrome c release, caspase-3-activation, decrease in oxygen consumption and NADH levels in mitochondria, chromatin condensation, autophagy process and apoptosis could be associated with this mitochondrial alteration after application of TiO2 NPs (Huerta-García et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro

Tománková

Hořáková

Harvanová

et al. 2015

Food and Chemical Toxicology

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Commercially manufactured nanomaterials are used massively for modification of products of everyday use, including products intended for children. Therefore their potential risks have to be ultimately studied. Aside from toxicity of nanomaterials with known specific parameters, the end-consumer is potentially endangered by materials with unknown specification. Commercially available products are not usually accompanied by parameter/specification sheet providing the consumer with sufficient chemico-physical parameters allowing the evaluation of possible toxic effects. The aim of this work was to evaluate the declared parameters of commercially available TiO2 and Ag NPs employing chemico-physical methods and consequently in vitro cytotoxicity and genotoxicity tests performed on non-cancer cell lines. Based on the results of our complex study we can conclude that the data provided by the producers are not in good agreement with the performed measurements. Furthermore, all tested NPs penetrated into the SVK14 cells and all NPs had significant effect on the kinetics of ROS production in all cell lines (note: the ROS production has not been established as the major mechanism of cell damage elicited by Ag NPs). The study revealed greater cytotoxic potential of Ag NPs in comparison with TiO2 NPs and all of the studied NPs caused significant DNA damage.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro

Tománková

Hořáková

Harvanová

et al. 2015

Food and Chemical Toxicology

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“…It has been reported that TiO 2 nanoparticles might result in apoptosis of PC12 cells by enhancing the intracellular ROS generation (Liu et al 2009). The cytochrome c release, caspase-3-activation, decrease of NADH levels in mitochondria, chromatin condensation, and apoptosis could be associated with mitochondrial alteration after treatment of TiO 2 nanoparticles (Huerta-García et al 2014). Some researchers suggested that ROS produced by nanoparticles could induce mitochondrial membrane permeability, ultrastructural mitochondrial damage, and disturb respiratory chain to trigger apoptosis (Park et al 2008;Botelho et al 2014).…”

Section: Controlmentioning

confidence: 99%

Size-dependent cytotoxicity of yttrium oxide nanoparticles on primary osteoblasts in vitro

Zhou

et al. 2016

J Nanopart Res

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Yttrium oxide nanoparticles are an excellent host material for the rare earth metals and have high luminescence efficiency providing a potential application in photodynamic therapy and biological imaging. In this study, the effects of yttrium oxide nanoparticles with four different sizes were investigated using primary osteoblasts in vitro. The results demonstrated that the cytotoxicity generated by yttrium oxide nanoparticles depended on the particle size, and smaller particles possessed higher toxicological effects. For the purpose to elucidate the relationship between reactive oxygen species generation and cell damage, cytomembrane integrity, intracellular reactive oxygen species level, mitochondrial membrane potential, cell apoptosis rate, and activity of caspase-3 in cells were then measured. Increased reactive oxygen species level was also observed in a size-dependent way. Thus, our data demonstrated that exposure to yttrium oxide nanoparticles resulted in a size-dependent cytotoxicity in cultured primary osteoblasts, and reactive oxygen species generation should be one possible damage pathway for the toxicological effects produced by yttrium oxide particles. The results may provide useful information for more rational applications of yttrium oxide nanoparticles in the future.

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“…Furthermore, Huerta-Garcia et al 67 found that TiO 2 NPs caused intense oxidative stress in both rat and human glial cells (C6 and U373 lines) by regulating changes in the cellular redox state and lipid peroxidation associated with a rise in the expression of glutathione peroxidase, catalase (CAT), and superoxide dismutase 2 (SOD2). TiO 2 NPs also induced morphology changes, mitochondrial damage, and increased mitochondrial membrane potential, indicating a cytotoxic effect of TiO 2 NPs on glial cells.…”

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confidence: 99%

Central nervous system toxicity of metallic nanoparticles

Feng¹,

Chen²,

Zhang³

et al. 2015

IJN

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Nanomaterials (NMs) are increasingly used for the therapy, diagnosis, and monitoring of disease- or drug-induced mechanisms in the human biological system. In view of their small size, after certain modifications, NMs have the capacity to bypass or cross the blood–brain barrier. Nanotechnology is particularly advantageous in the field of neurology. Examples may include the utilization of nanoparticle (NP)-based drug carriers to readily cross the blood–brain barrier to treat central nervous system (CNS) diseases, nanoscaffolds for axonal regeneration, nanoelectromechanical systems in neurological operations, and NPs in molecular imaging and CNS imaging. However, NPs can also be potentially hazardous to the CNS in terms of nano-neurotoxicity via several possible mechanisms, such as oxidative stress, autophagy, and lysosome dysfunction, and the activation of certain signaling pathways. In this review, we discuss the dual effect of NMs on the CNS and the mechanisms involved. The limitations of the current research are also discussed.

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Titanium dioxide nanoparticles induce strong oxidative stress and mitochondrial damage in glial cells

Cited by 168 publications

References 53 publications

Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro

Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro

Size-dependent cytotoxicity of yttrium oxide nanoparticles on primary osteoblasts in vitro

Central nervous system toxicity of metallic nanoparticles

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