Titanium dioxide nanoparticles modulate the toxicological response to cadmium in the gills of Mytilus galloprovincialis

Genomic and chromosomal damage in the marine mussel Mytilus galloprovincialis: Effects of the combined exposure to titanium dioxide nanoparticles and cadmium chloride

“…1). DLS analysis confirmed the formation of agglomerates of n-TiO 2 of micrometric size within the first minute after sonication, with values of polydispersity index ranging from 0.295 (±0.028) to 0.383 (±0.041) and Z-average ranging from 972 (±35.37) to 1448 (±25.72) nm (Della Torre et al, 2015a). All chemicals were purchased from Sigma.…”

“…Primary n-TiO 2 characterization was performed by TEM. Secondary characterization of n-TiO 2 in ASW was carried out by DLS analysis and Differential Centrifuge Sedimentation (DCS) (Della Torre et al, 2015a). TEM observation of n-TiO 2 showed spheroid irregular shape and a mean particle diameter of 24 ± 7 nm ( Fig.…”

“…Nanoparticled TiO 2 genotoxicity has been assessed in mussel haemocytes as oxidative DNA damage (D'Agata et al, 2014) and in mussel gill cells as micronuclei formation. An overall picture of the cellular responses to the combined exposure to n-TiO 2 and CdCl 2, including DNA primary damage, was assessed in the Mediterranean mussel by our research group (Della Torre et al, 2015a). In the present study we focused the investigation on genomic and chromosomal damage potentially associated to n-TiO 2 and CdCl 2 in Mytilus galloprovincialis both after single and combined exposure.…”

Toxicological effects of benzo(a)pyrene, DDT and their mixture on the green mussel Perna viridis revealed by proteomic and metabolomic approaches

“…Apart from their abundance and wide distribution within local water bodies, their sedentary and filter-feeding characteristics ensure them to be an ideal pollution indicator species (Siu et al, 2004). Gills are the first site of contaminant entry and detoxification (Della Torre et al, 2015), and also, as the main entry organ of waterborne toxicants, either dissolved or bound to particulate matter (especially in filter-feeders as bivalves), are more prone to experience oxidative stress than other tissues which should imply that antioxidant mechanisms have to be more rapid and efficient in this tissue than in others (Regoli and Principato, 1995). Fedato et al (2010) showed that gill cells proved to be more sensitive than hemocytes to the genotoxic effects of gasoline water soluble fraction (GWSF), also Rank and Jensen (2003) observed that gill cells were more sensitive to DNA damage detected by comet assay than hemolymph cells to the genotoxic effects of methylmethanesulfonate (MMS).…”

Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells

“…Therefore, the study of interactions among contaminants has a practical importance in toxicology (Goldoni and Johansson 2007), particularly for novel contaminants such as AgNP. A number of studies have investigated the interactions of nanoparticles and widespread environmental xenobiotics, such as metals, polycyclic aromatic hydrocarbons, and pesticides in different organisms (Guo et al 2013;Ferreira et al 2014;Della Torre et al 2015;Deville et al 2016). Deville et al (2016), for instance, showed that interaction of gold nanoparticles with nickel sulfate led to competitive effects on dendritic cell maturation, unlike separate exposures.…”