Tithonia diversifolia and its main active component tagitinin C induce survivin inhibition and G2/M arrest in human malignant glioblastoma cells

Liao, May-Hua; Lin, Wen‐Chieh; Wen, Hsiao-Chuan; Pu, Hsiao‐Fung

doi:10.1016/j.fitote.2010.11.002

Cited by 36 publications

(31 citation statements)

References 36 publications

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“…This compound may have the same chemical structure with the active compound previously isolated by Soeparto 10 as indicated by the same Rf value (0.3) and the same band using visualisation at UV 254 nm. Furthermore, this active compound is suggested as tagitinin C (1 , 2 -epoxytaginin C) that previously isolated by Liao et al 14 The cytotoxicity testing showed that the isolated active compound has potential cytotoxic activity against WiDr cell line with an IC 50 value of 3.75 µg/mL. This cytotoxic activity of this isolated active compound is higher than 5-FU (>40 µg/mL), as positive control, indicating that the isolated active compound is more active against WiDr cell line (TABLE 1).…”

Section: Discussionmentioning

confidence: 86%

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The effect of active compound isolated from the leaves of kembang bulan [ Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line

2013

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Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients in Indonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs. Molecular targets for anticancer drug have been identified including genes associated with cell cycle control and angiogenesis. Previously, an active and selective compound against WiDr from Tithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate the effect of the isolated active compound from T. diversifolia on the WiDr cell cycle and angiogenesis. Isolation of the active compound was performed by preparative thin layer chromatography (TLC) method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI). Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGF monoclonal antibody. The results showed that the effect of the isolated active compound on the WiDr cell cycle depended on the concentration and the incubation time periods. At concentration of 4 µg/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hours incubation and G1 phase after 72 hours incubation. While at concentration of 8 µg/mL, it clearly inhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, the isolated active compound at concentration of 4 µg/mL significantly inhibited the VEGF expression until 47.38% compared to control. In conclusion, the isolated active compound from T. diversifolia inhibited cell cycle and angiogenesis of WiDr cell. ABSTRAKKanker kolorektal merupakan tumor ganas paling umum ke 10 yang dijumpai pada pasien rawat inap di rumah sakit di Indonesia. Pendekatan terkini dalam pengembangan antikanker adalah penemuan obat dengan target kerja di tingkat molekuler. Target molekuler untuk obat antikanker telah diidentifikasi di antaranya gen yang berperan dalam siklus sel dan angiogenesis. Penelitian sebelumnya menunjukkan sebuah senyawa aktif dan selektif terhadap sel WiDr dari Tithonia diversifolia (Hemsley) A. telah berhasil diisolasi. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian senyawa aktif hasil isolasi dari T. diversifolia terhadap siklus sel dan angiogenesis sel WiDr. Isolasi senyawa aktif dilakukan dengan kromatografi lapis tipis (KLT) preparatif. Siklus sel WiDr dianalisis dengan flowsitometri menggunakan propidium iodida (PI). Efek angiogenesis dikaji menggunakan metode imunositokimia menggunakan antibodi monoklonal anti-human VEGF. Hasil penelitian menunjukkan efek senyawa aktif hasil isolasi terhadap siklus sel WiDr tergantung pada konsentrasi dan masa inkubasi. Pada konsentrasi 4 µg/mL, senyawa ini menghambat siklus sel WiDr fase SubG1 setelah inkubasi 36 dan 48 jam dan fase G1 setelah inkubasi 72 jam. 102J Med Sci, Volume 45, No. 3, September 2013: 101-111 Sedangkan pada konsentrasi 8 µg/mL, senyawa ini dengan jelas menghambat siklus sel WiDr fase G1 setelah inkubasi 36, 48 dan 72 jam. Lebih lanjut senyawa aktif hasil isolasi pada konsentrasi pada 4 µg/mL secara nyata mengh...

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Section: Discussionmentioning

confidence: 86%

“…14 Furthermore, Garcia and Delgado 15 isolated tagitinin A and tagitinin C from T. diversifolia that exhibited a cytotoxic effect on HCT-15 cells. While Gu et al 16 reported that tagitinin C shows antiproliferation activity on human colon cancer (Col2) cells.…”

Section: Introductionmentioning

confidence: 99%

The effect of active compound isolated from the leaves of kembang bulan [ Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line

2013

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“…261 The biosynthesis and localization of parthenolide in different tissues and developmental stages of Tanacetum parthenium have been analyzed, showing that glandular trichomes are the place where the biosynthesis and accumulation of this lactone occur. 266 The anti-human glioblastoma 267,268 and anti-ulcer activities of the germacranolide tagitenin C 272 have been described. The anti-bacterial and cytotoxic activities of the sesquiterpene lactones cnicin and onopordopicrin have been reported.…”

Section: Germacranementioning

confidence: 99%

Natural sesquiterpenoids

Fraga¹

2012

Nat. Prod. Rep.

105

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“…[10] and Liao et al . [11] indicated that T. diversifolia extract and the isolated tagitinin C act as chemotherapeutic adjuvants on the glioblastoma cells. The activity against Plasmodium falciparum [12] was also reported by the presence of tagitinin C as well as the gastroprotective activity.…”

Section: Introductionmentioning

confidence: 99%

Comparison of ultrasound-assisted extraction and dynamic maceration over content of tagitinin C obtained from Tithonia diversifolia (Hemsl.) A. gray leaves using factorial design

et al. 2017

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Background:Tithonia diversifolia belongs to the Asteraceae family. The leaves of T. diversifolia have been studied lately because of the presence of tagitinin C.Objective:Looking for an easy and inexpensive method to extract tagitinin C from T. diversifolia leaves, this work aims to conduct a screening to evaluate the influence of different experimental factors using the dynamic maceration and ultrasound-assisted extraction methods with 23 factorial design based on response surface methodology in enhancing this chemical marker extraction.Materials and Methods:The experimental factors were: extraction time (ET) of 30 and 60 minutes, solid: liquid ratio (SLR) of 5 and 10 grams/grams and ethanolic strength (ES) 48 and 96% (w/w). The experiments were done tripled. The content of tagitinin C in each produced extract was quantified by HPLC method.Results:The highest concentrations of tagitinin C obtained under the experimental design were 0.53 mg/mL and 0.71 mg/mL, respectively for dynamic maceration (DM) and ultrasound-assisted extraction (UAE) from Tithonia diversifolia powdered leaves. For the UAE method, the main parameter for higher contents of tagitinin C was the solid: liquid ratio, followed by the ethanolic strength, and the extraction time was not significant for this method. As for the DM method, all the parameters (SLR, ES, and ET) were significant for a higher content of tagitinin C.Conclusion:Based on the obtained results, it was revealed that the ultrasound-assisted extraction was more effective than dynamic maceration for tagitinin C extraction from T. diversifolia powdered leaves.Summary Tithonia diversifolia leaves possess tagitinin C, a sesquiterpene lactone, as an important secondary metabolite with several biological activities, such as antimalarial, gastroprotective, chemotherapeutic adjuvants, and toxic activities.Ultrasound-assisted extraction was more effective to obtain higher levels of tagitinin C when compared with dynamic maceration extraction.Factorial design can be employed as a screening tool to find the effects of factors investigated in the extraction processes. Abbreviation used: DME: dynamic maceration extraction, UAE: ultrasound-assisted extraction, ET: extraction time, ES: ethanolic strength, SLR: solid:liquid ratio, Tag C: tagitinin C, HPLC: high-performance liquid chromatography.

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Tithonia diversifolia and its main active component tagitinin C induce survivin inhibition and G2/M arrest in human malignant glioblastoma cells

Cited by 36 publications

References 36 publications

The effect of active compound isolated from the leaves of kembang bulan [ Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line

The effect of active compound isolated from the leaves of kembang bulan [ Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line

Natural sesquiterpenoids

Comparison of ultrasound-assisted extraction and dynamic maceration over content of tagitinin C obtained from Tithonia diversifolia (Hemsl.) A. gray leaves using factorial design

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