TLR2 signaling may play a key role in the probiotic modulation of intestinal microbiota in grouper Epinephelus coioides

Sun, Yun‐Zhang; Xia, H.-Q.; Yang, Hongling; Wang, Yilei; Zou, Wen-Chao

doi:10.1016/j.aquaculture.2014.03.042

Cited by 55 publications

(34 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, significantly enhanced expression of TLR5 was observed in both head kidney and intestine of fish fed heat‐killed B. clausii DE5, which may indicate that the cell components of heat‐killed B. clausii DE5 has been recognized by TLRs. Consequently, enhanced expression of IL‐1β was observed in fish fed heat‐killed B. clausii DE5, which is consistent with previous reports that heat‐killed probiotics enhanced the expression of IL‐1β in Japanese puffer fish ( Takifugu rubripes ) (Biswas et al., ), grouper ( E. coioides ) (Sun et al., ) and Atlantic cod ( Gadus morhua ) (Lazado & Caipang, ). At the same time, significantly upregulated expression of IL‐8 in E. coioides fed heat‐killed B. clausii DE5 was observed as IL‐1β stimulates the IL‐8 release (Laing & Secombes, ).…”

Section: Discussionsupporting

confidence: 90%

“…The proximate analysis of the basal diet according to the AOAC method was as follows: 506.3 g/kg crude protein, 103.2 g/kg crude lipid, 98.6 g/kg ash, 1.84 g/kg crude fibre and 944.9 g/kg dry matter (AOAC ). Control diet was prepared as described by (Sun et al., ). Live and heat‐inactivated B. clausii DE5 suspensions were sprayed into the basal diet and mixed part‐by‐part in a mixer to obtain a final probiotic concentration of 1.0 × 10 8 CFU/g.…”

Section: Methodsmentioning

confidence: 99%

“…Considering the rapid demand for high quality animal protein and the trend of environmentally friendly aquaculture, the application of probiotics has been suggested as an alternative method for the prevention and control of various diseases in aquaculture (Lazado & Caipang, ; Lazado, Caipang, & Estante, ; Newaj‐Fyzul, Al‐Harbi, & Austin, ; Safari et al, ). Several studies have demonstrated the potential application of probiotics in grouper aquaculture (Huang, Wu, & Chi, ; Liu, Chiu, Wang, & Cheng, ; Reyes‐Becerril et al., ; Son et al., ; Sun, Xia, Yang, Wang, & Zou, ; Sun, Yang, Huang, Ye, & Zhang, ; Sun et al., ). Dietary supplementation of Pediococcus pentosaceus enhanced the growth rate and the erythrocyte counts, elevated proliferation of leucocytes and phagocytic activity of head kidney phagocytes against Vibrio anguillarum infection and protected the orange‐spotted grouper ( Epinephelus coioides ) from vibriosis (Huang et al., ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Supplementation of heat-inactivated Bacillus clausii DE5 in diets for grouper, Epinephelus coioides, improves feed utilization, intestinal and systemic immune responses and not growth performance

et al. 2017

Self Cite

View full text Add to dashboard Cite

In recent years, more and more attentions have been paid to the development and application of probiotics in aquaculture, and viable probiotics have been extensively studied, while rare information was available about inactivated probiotics in aquaculture. Therefore, in this study, a feeding trial was designed to investigate the effect of dietary supplementation of heat-inactivated probiotic Bacillus clausii DE5 on growth performance, immune response and key immune genes expression in head kidney and intestine in grouper Epinephelus coioides. Fish were fed for 60 days with control diet (C) and two experimental diets containing 1.0 × 10 8 CFU/g live (T1) and heatinactivated (T2) B. clausii DE5, respectively. The probiotic treatments did not affect the final weight, weight gain (WG) and specific growth rate (SGR) of E. coioides at days 30 and 60 (p > .05), while both heat-inactivated and live B. clausii DE5 significantly decreased the feed intake and feed conversion ratio (FCR) at day 60 (p < .05). Serum lysozyme activity and complement C3 level in the two probiotic treatments were significantly higher than those in the control (p < .05). The lysozyme activity and complement C3 level at day 60 were significantly higher than those at day 30 (p < .05), while no significant interaction effect between diet and administration date was observed.Moreover, the heat-inactivated B. clausii DE5 significantly improved the expression of TLR5, pro-inflammatory cytokines (IL-8 and IL-1β) and TGF-β1 in head kidney and intestine (p < .05), while the live probiotic did not show any significant effect on the expression of these key immune-related genes in head kidney and intestine. These results indicate that dietary supplementation of heat-inactivated B. clausii DE5 effectively improved feed utilization and both the local and systemic immune responses of E. coioides. K E Y W O R D SBacillus clausii, Epinephelus coioides, growth performance, heat-inactivated probiotic, intestinal immunity, systemic immunity

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Supplementation of heat-inactivated Bacillus clausii DE5 in diets for grouper, Epinephelus coioides, improves feed utilization, intestinal and systemic immune responses and not growth performance

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, to our knowledge, the literature referring to the evaluation of the effectiveness of heat‐inactivated probiotics in E. coioides is limited (Sun et al . ).…”

Section: Introductionmentioning

confidence: 97%

Effects of dietary live or heat-inactivated autochthonousBacillus pumilusSE5 on growth performance, immune responses and immune gene expression in grouperEpinephelus coioides

et al. 2015

View full text Add to dashboard Cite

To evaluate the possible dietary application of live and heat-inactivated probiotic Bacillus pumilus SE5 in grouper Epinephelus coioides, juveniles (14.6 AE 0.2 g) were fed either a basal control diet (without probiotic) or the basal diet supplemented with 1.0 9 10 8 CFU g À1 live (T1) and heat-inactivated B. pumilus SE5 (T2). The heat-inactivated probiotic significantly improved the final weight, weight gain (WG) and specific growth rate (SGR) at day 60 and significantly decreased the feed conversion ratio (FCR) at day 30 and 60, while the viable probiotic significantly decreased the FCR at day 60 (P < 0.05). Phagocytic activity, serum complement C3 and IgM levels as well as SOD activity elevated significantly in fish fed the heat-inactivated probiotic for 60 days (P < 0.05). Furthermore, the heatinactivated probiotic remarkably up-regulated expression of TLR2 and pro-inflammatory cytokines (IL-8 and IL-1b) in head kidney (P < 0.05), but the viable probiotic failed to do so. These results indicated that heat-inactivated B. pumilus SE5 can effectively improve the growth performance and immune responses of E. coioides.

show abstract

“…The grouper aquaculture industry has been well developed in China and South‐East Asia countries, resulting from increasing market demand (Ma, Wang, Liao & Yang, ; Sun, Xia, Yang, Wang & Zou, ). Unfortunately, the rapid development of intensive grouper culture leads to incidences of infectious disease caused by bacteria, viruses and parasites that have become more and more frequent which result in serious economic losses in farms and hatcheries (Son et al., ; Harikrishnan et al., , ).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation of probiotics on the growth, activities of digestive and non-specific immune enzymes in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Feng

Tian

et al. 2017

Aquac Res

View full text Add to dashboard Cite

Effects of Bacillus cereus BC‐01, Lactobacillus acidophilus LAG01, Clostridium butyricum CBG01 and their combinations as supplementation on the growth performance, digestive enzyme activities and serum non‐specific immunity of hybrid grouper (Epinephelus lanceolatus ♂×E. fuscoguttatus ♀) were assessed. Seven different diets, that is one control diet (basal feed without any probiotics, CT) and six treatment diets containing single B. cereus (Bs), L. acidophilus (Ls) and C. butyricum (Cs) at 1.0 × 109 cfu/kg feed, and also their combinations in equal proportion at 1.0, 2.0 and 3.0 × 109 cfu/kg feed (BLC1, BLC2 and BLC3) were prepared respectively. After 60‐day feeding trial, the final weight, specific growth rate,food consumption, food conversion efficiency and apparent digestibility coefficient of fish in Ls and BLC3 were significantly higher compared with the control (p < .05). The activities of pepsin and trypsin in the intestine of fish for Ls and BLC3 were significantly higher relative to the control (p < .05). Relative to controls, significantly enhanced amylase and lipase activities in proximal intestine except for Cs and BLC1 and lipase activities in distal intestine except for Cs were observed (p < .05). Meanwhile, activities of superoxide dismutase in the serum of fish for all treatments, lysozyme and catalase in Ls and BLC3, and glutathione peroxidase except for Cs were significantly enhanced (p < .05). Based on the above, dietary supplementation of single L. acidophilus at 1.0 × 109 cfu/kg or combination of three strains at 3.0 × 109 cfu/kg for hybrid grouper is recommended.

show abstract

TLR2 signaling may play a key role in the probiotic modulation of intestinal microbiota in grouper Epinephelus coioides

Cited by 55 publications

References 30 publications

Supplementation of heat-inactivated Bacillus clausii DE5 in diets for grouper, Epinephelus coioides, improves feed utilization, intestinal and systemic immune responses and not growth performance

Supplementation of heat-inactivated Bacillus clausii DE5 in diets for grouper, Epinephelus coioides, improves feed utilization, intestinal and systemic immune responses and not growth performance

Effects of dietary live or heat-inactivated autochthonousBacillus pumilusSE5 on growth performance, immune responses and immune gene expression in grouperEpinephelus coioides

Effects of dietary supplementation of probiotics on the growth, activities of digestive and non-specific immune enzymes in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Contact Info

Product

Resources

About