2014
DOI: 10.1016/j.vetimm.2014.01.005
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TLR4 is constitutively expressed in chick thymic epithelial cells

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Cited by 11 publications
(11 citation statements)
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“…For immunochemistry, the antibodies of TLR4 (1:50), LC3A/B (1:50), PCNA (1:100), PCK (1:50) and S100 (1:50) were used, which either target the amino acid sequences sharing high identity (>70%) with those found in ostriches, or their applicability for birds are declared by each company ( S1 Table ). The immunochemistry staining was performed as described [ 16 ]. For apoptotic staining, a mouse IgM anti-ssDNA monoclonal antibody (1:10; EMD Millipore, Billerica, USA) was used.…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…For immunochemistry, the antibodies of TLR4 (1:50), LC3A/B (1:50), PCNA (1:100), PCK (1:50) and S100 (1:50) were used, which either target the amino acid sequences sharing high identity (>70%) with those found in ostriches, or their applicability for birds are declared by each company ( S1 Table ). The immunochemistry staining was performed as described [ 16 ]. For apoptotic staining, a mouse IgM anti-ssDNA monoclonal antibody (1:10; EMD Millipore, Billerica, USA) was used.…”
Section: Methodsmentioning
confidence: 99%
“…The expressions of TLR4, LC3A/B and PCNA were measured using integrated optical density (IOD) ratio as described [ 16 ], and the total numbers of ssDNA positive cell per 1 × 10 5 μm 2 in thymic cortex were quantified.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…For example, TLR4 signaling plays a key role in immune cell recruitment in the MG thymus (Cordiglieri et al 2014), and TLR7 and TLR9 are also involved in the pathogenesis of the MG thymus (Cavalcante et al 2016). TLR4 is constitutively expressed on TECs (Huang et al 2014), and the expression of TLR4 was enhanced on the TECs of the involuted thymuses of myasthenic patients (Bernasconi et al 2005). The indirect effect of endotoxin stimulation on thymic involution caused alterations in the gene expression downstream of TLR3 and TLR4 (Fig.…”
Section: Mechanisms Of Ati Role Of Tlrs and Atimentioning
confidence: 99%
“…Sections were then reacted with 5% normal goat serum (Thermo Scientific, Rockford, USA) for 10 min at 37 • C to prevent any non-specific reactions, and incubated with a rabbit polyclonal antibody to human TLR4 (1:100 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at 4 • C overnight. This antibody to TLR4 was raised against a peptide near the C-terminus of human TLR4, and has previously been used to identify TLR4 in chickens (Huang et al, 2014). After incubation with the primary antibody, sections were washed in PBS three times, and incubated for 20 min with goat anti-rabbit IgG antibody (Thermo Scientific, Rockford, USA) labeled with peroxidase.…”
Section: Immunohistochemical Stainingmentioning
confidence: 99%