TMT-based quantitative membrane proteomics identified PRRs potentially involved in the perception of MSP1 in rice leaves

Min, Cheol Woo; Jang, Jeong Woo; Lee, Gi Hyun; Gupta, Ravi; Yoon, Jinmi; Park, Hyun Ji; Cho, Hye Sun; Park, Sang Ryeol; Kwon, Soon-Wook; Cho, Lae-Hyeon; Jung, Kyungsook; Kim, Yujin; Wang, Yiming; Kim, Sun Tae

doi:10.1016/j.jprot.2022.104687

Cited by 14 publications

(25 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The soluble protein fraction was precipitated with 4 volumes of 12.5% TCA/acetone containing 0.07% (v/v) β -mercaptoethanol. Finally, precipitated microsomal and soluble proteins were washed with 80% acetone containing 0.07% (v/v) β -mercaptoethanol [1] and used for in-solution trypsin digestion by filter-aided sample preparation (FASP) method [5 , 6] . In brief, acetone precipitated proteins (300 μg) were dissolved in denaturation buffer [4% SDS and 100 mM DTT in 100 mM TEAB, pH 8.5], sonicated for 3 min, and incubated at 99 °C for 30 min.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…TMT labeling of the digested peptides was carried out using TMT-6plex kit following manufacturer's protocol [1 , 5] . Briefly, each TMT reagent was re-suspended in 120 μL of anhydrous ACN and subsequently 25 μL of each TMT reagent was added in samples.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“… Data reported here is associated with the article entitled “TMT-based quantitative membrane proteomics identified pattern recognition receptors (PRRs) potentially involved in the perception of MSP1 in rice leaves” [1] . PAMP-triggered immunity (PTI) constitutes the first layer of plant innate immunity against pathogen infection.…”

mentioning

confidence: 99%

“…However, identification of PRRs involved in the recognition of MSP1 has not been achieved so far. In this manuscript, we carried out comprehensive proteomic profiling to investigate the potential PRRs and MSP1 induced signaling cascades using MSP1 overexpressed transgenic rice by TMT-labeling based quantitative analysis with QExactive TM Orbitrap High-Resolution Mass Spectrometer [1] .…”

mentioning

confidence: 99%

“…Park, S. Kwon, L. Cho, K. Jung, Y. Kim, Y. Wang, S.T. Kim, TMT-based quantitative membrane proteomics identified PRRs potentially involved in the perception of MSP1 in rice leaves, J. Proteomics 267 (2022) 104687 [1] .…”

mentioning

confidence: 99%

See 4 more Smart Citations

TMT-based quantitative proteome data of MSP1 overexpressed rice

et al. 2023

Self Cite

View full text Add to dashboard Cite

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

TMT-based quantitative proteome data of MSP1 overexpressed rice

et al. 2023

Self Cite

View full text Add to dashboard Cite

Editing of rice (Oryza sativa L.) OsMKK3 gene using CRISPR/Cas9 decreases grain length by modulating the expression of photosystem components

et al. 2023

View full text Add to dashboard Cite

Grain size is one of the most important agronomic traits for grain yield determination in rice. To better understand the proteins that are regulated by the grain size regulatory gene OsMKK3, this gene was knocked out using the CRISPR/Cas9 system, and tandem mass tag (TMT) labeling combined with liquid chromatograph‐tandem mass spectrometry analysis was performed to study the regulation of proteins in the panicle. Quantitative proteomic screening revealed a total of 106 differentially expressed proteins (DEPs) via comparison of the OsMKK3 mutant line to the wild‐type YexiangB, including 15 and 91 up‐regulated and down‐regulated DEPs, respectively. Pathway analysis revealed that DEPs were enriched in metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, and photosynthesis. Strong interactions were detected among seven down‐regulated proteins related to photosystem components in the protein‐protein interaction network, and photosynthetic rate was decreased in mutant plants. The results of the liquid chromatography‐parallel reaction monitoring/mass spectromery analysis and western blot analysis were consistent with the results of the proteomic analysis, and the results of the quantitative reverse transcription polymerase chain reaction analysis revealed that the expression levels of most candidate genes were consistent with protein levels. Overall, OsMKK3 controls grain size by regulating the protein content in cells. Our findings provide new candidate genes that will aid the study of grain size regulatory mechanisms associated with the mitogen‐activated protein kinase (MAPK) signaling pathway.

show abstract