TMT-Based Quantitative Proteomics Analyses Reveal the Antibacterial Mechanisms of Anthocyanins from Aronia melanocarpa against Escherichia coli O157:H7

Abstract

Aronia melanocarpa anthocyanins (AMAs), as natural plant extracts, can control pathogens and are attracting increasing attention. In this study, a tandem mass tag (TMT) quantitative proteomics method combined with multiple reaction monitoring (MRM) was used to explore the antibacterial mechanism of AMAs against Escherichia coli at the protein level. The results showed that 1739 proteins were identified in E. coli treated with AMAs, of which 628 were altered, including 262 downregulated proteins and 366 upregulated proteins. Bioinformatics analysis showed that these differentially expressed proteins have different molecular functions and participate in different molecular pathways. AMAs can affect E. coli protein biosynthesis, DNA replication and repair, oxidative stress response, peptidoglycan biosynthesis, and homeostasis. These pathways induce morphological changes and cell death. The results of this study help understand the molecular mechanism of the inhibitory effect of AMAs on food-borne pathogens and provide a reference for further development of plant-derived antimicrobial agents.

A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

“…The proteomics method was carried out according to the previous description. 21,22 In brief, E. coli O157:H7 cells were added with lysis buffer and centrifuged to obtain the supernatant. DTT was added to the sample, and iodoacetamide was added after a water bath at 56 °C for 1 h. 30 μg of the sample was added to the ultrafiltration tube, and the waste liquid was discarded after centrifugation.…”

“…Previous reports have shown that AMAs can enter Escherichia coli O157:H7 cells through the bacillary mechanosensitive channel and affect the expression of proteins involved in oxidative stress, protein biosynthesis, DNA replication and repair and other processes. 21,22 Furthermore, metabolomics and proteomics data were integrated. First, the pathway results were classified and analyzed, including metabolism, environmental information processing, cellular processes, human diseases, and genetic information processing.…”

TMT-Based Quantitative Proteomics and Non-targeted Metabolomic Analyses Reveal the Antibacterial Mechanism of Hexanal against Vibrio parahaemolyticus

“…However, the overuse of antibiotics in cultivating fishery and clinical treatment resulted in antibiotic resistance. 8 In recent years, compounds of natural origins with antimicrobial activity have attracted growing interest of researchers. These substances are often recognized as safe or low-toxic and are less likely to induce drug resistance.…”

“…Proteomics and metabolomics are effective approaches to investigate the changes of proteins and metabolic profiles in bacteria caused by environmental stress, and which have been extensively used to elucidate the antimicrobial mechanism of natural compounds. 8,19,20 In this study, the minimum inhibitory concentration (MIC) value, intracellular ATP concentration, membrane permeability, and ultrastructure changes were determined to assess the antibacterial effect of hexanal against V. parahaemolyticus. TMT-based quantitative proteomics and non-targeted metabolomics were performed to reveal the associated mode of action of hexanal against V. parahaemolyticus.…”

“…parahaemolyticus control. However, the overuse of antibiotics in cultivating fishery and clinical treatment resulted in antibiotic resistance . In recent years, compounds of natural origins with antimicrobial activity have attracted growing interest of researchers.…”