Tn5090-like class 1 integron carrying blaVIM-2 in a Pseudomonas putida strain from Portugal

Abstract

Three Pseudomonas putida strains containing bla(VIM-2) were isolated from an inanimate surface of a female ward sanitary facility in the Hospital Infante D. Pedro, Aveiro. A novel class 1 integron was found in strain Pp2 (aacA4/bla(VIM-2)/aac6'-IIc disrupted by an insertion sequence IS1382), and strain Pp1 was found to carry a class 1 integron (aacA7/bla(VIM-2)/aacC1/aacA4), which is described for the first time in this species. Strain PF1 carries a class 1 integron associated with a Tn5090-like transposon, constituting the first finding of this type of arrangement in a strain from Portugal. This association highlights further dissemination of bla(VIM-2) in environmental hospital isolates.

Presence of VIM-Positive Pseudomonas Species in Chickens and Their Surrounding Environment

“…This gene arrangement, in combination with intl1, is involved in the transfer of a bla VIM-2 -carrying cassette in clinical Pseudomonas spp. (16)(17)(18)(19). A 9,875-bp fragment containing the bla VIM-2 -like gene was observed in three P. putida strains that shared 99.9% sequence identity.…”

“…Similar to the spread of bla VIM-2 in bacteria of clinical origin, the mobile genetic elements, such as Tn5090, Tn1403, intl1, and IS6100, may play an important role in the dissemination of the bla VIM gene and its conserved flanking regions in bacteria among food animals (16,17,24). Our study has found MBL-producing Pseudomonas isolates not only in the livestock but also in their surrounding environment, suggesting that surveillance of carbapenemase-producing bacteria in livestock and their surrounding environment is urgently needed.…”

A novel class 1 integron array carrying bla VIM-2 genes and a new insertion sequence in a Pseudomonas aeruginosa strain isolated from a Spanish hospital

“…Several studies have described class 1 integrons containing the bla VIM-2 gene cassette (Walsh et al, 2005;Santos et al, 2010;Samuelsen et al, 2010;Hammami et al, 2010), but to our knowledge, this is the first report of the double detection of bla VIM-2 gene cassettes in the variable region of the same integron in P. aeruginosa. In addition, the presence of a new insertion sequence in the surrounding environment could be implicated in the mobilization of these gene cassettes.…”

Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa Reveals Tn 6352 , a Novel Putative Transposon Associated with Mobilization of the bla VIM-2 -Harboring In58 Integron

“…The bla VIM-2 gene has been commonly associated with chromosomally located Tn402-like class 1 integrons (3)(4)(5)(6)(7)(8). This gene also has been less frequently linked to plasmids, usually smaller than 100 kb, and cannot be transferred by conjugation, at least not to Escherichia coli (1,9).…”

“…In P. aeruginosa, bla VIM-2 is most often associated with class 1 integrons containing additional antibiotic resistance genes (3)(4)(5)(6)(7)(8)(9)(10)(11). The bla VIM-2 gene has been commonly associated with chromosomally located Tn402-like class 1 integrons (3)(4)(5)(6)(7)(8).…”