TNF-Binding domain of the variola virus CrmB protein synthesized in Escherichia coli cells effectively interacts with human TNF

An unusually high production of cytokines or chemokines as well as increased complement activation can drive development of chronic inflammatory autoimmune diseases. State-of-the-art biological therapies, recombinant receptors, or specific antibodies that target immune and inflammatory mediators are now effectively used. However, these newer drugs are not equally effective for all patients and can cause adverse effects, making the search for new immunomodulatory proteins of great importance. The poxviruses--first and foremost, the variola (smallpox) virus, which is highly pathogenic in man--code for numerous highly evolved and extraordinarily effective immunomodulatory proteins that bind cytokines, chemokines, and proteins of the complement system. The discovery of and investigation into immune modulators from the variola virus has great potential for guiding new and effective drugs for autoimmune diseases.

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Immunomodulating Drugs Based on Poxviral Proteins

Shchelkunova

Щелкунов

2016

BioDrugs

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Construction of Artificial TNF-Binding Proteins Based on the 10th Human Fibronectin Type III Domain Using Bacterial Display

Shingarova

Petrovskaya

Zlobinov

et al. 2018

Biochemistry Moscow

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Construction of antibody mimetics on the base of alternative scaffold proteins is a promising strategy for obtaining new products for medicine and biotechnology. The aim of our work was to optimize the cell display system for the 10th human fibronectin type III domain (Fn3) scaffold protein based on the AT877 autotransporter from Psychrobacter cryohalolentis K5 and to construct new artificial TNF-binding proteins. We obtained a Fn3 gene combinatorial library and screened it using the bacterial display method. After expression of the selectedFn3 variants in Escherichia coli cells and analysis of their TNF-binding activity, we identified proteins that display high affinity for TNF and characterized their properties.

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Gene therapy of arthritis

2016

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TNF-Binding domain of the variola virus CrmB protein synthesized in Escherichia coli cells effectively interacts with human TNF

Cited by 5 publications

References 13 publications

Immunomodulating Drugs Based on Poxviral Proteins

Immunomodulating Drugs Based on Poxviral Proteins

Construction of Artificial TNF-Binding Proteins Based on the 10th Human Fibronectin Type III Domain Using Bacterial Display

Gene therapy of arthritis

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