To assess weather the external application of traditional Chinese medicine can promote the trabecular bone regeneration and callus fibrous transformation by regulating IL-1, BMP2, TNF-α, TGF and other serum biochemical index AKP.

Abstract

Objective To assess weather the external application of traditional Chinese medicine can promote the trabecular bone regeneration and callus fibrous transformation by regulating IL-1, BMP2, TNF-α, TGF and other serum biochemical index AKP. Methods By constructing a rabbit model of closed fracture, the effects of different stages of external application of traditional Chinese medicine on inflammatory factors, serum biochemical indexes and bone marrow morphology were observed.. Results The expressions of inflammatory factors IL-1 and TNF-α in the closed fracture model after external application of traditional Chinese medicine 1, 2, 3 and positive drug treatment were significantly decreased (P < 0.05). The results of HE staining showed that after the 1, 2 and 3 stage of external application of traditional Chinese medicine and the positive drug treatment, new bone trabeculae grew into the bone marrow cavity, granulation tissue formed at the fracture end, and the original callus formed part of the area transformed into fibrous callus. The results of immunohistochemistry showed that the levels of BMP2, TGF and FGF increased during the treatment of bone marrow neogenesis with external application of traditional Chinese medicines in phases 1, 2 and 3 and positive drugs. (P < 0.05). X-ray results showed that the radial fracture line disappeared and gradually recovered after the treatment of Chinese medicine 1, 2, 3 and positive medicine. Conclusion External application of traditional Chinese medicine can promote trabecular bone regeneration and callus fibrous transformation by regulating inflammatory factors IL-1 and TNF-α, serum biochemical index AKP, and regulating the expression of BMP2, TGF and FGF levels.