To disaggregate or not to disaggregate, injury and cell disaggregation, transient or permanent?

Waymouth, Charity

doi:10.1007/bf02615343

Cited by 149 publications

(48 citation statements)

References 128 publications

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“…However, according to Schwyzer et al [8] the adrenal cortex could be triggered only by the main core, that is by ACTH-(4-10), or ACTH-(5-10), thus only one message sequence was supposed; however, they did not test shorter fragments. In the present experiments adrenal cortical cells could be stimulated also by ACTH- (11)(12)(13). Accordingly, we suggest that two message sequences exist also for steroidogenesis.…”

Section: Discussionsupporting

confidence: 71%

“…The present experiments were designed to investigate which part of the molecule induces these effects. To this end the effect of synthetic ACTH/~-MSH fragments: ACTH-(4-7), ACTH-(6-10), ACTH-(7-10), ACTH-(4-10) and ACTH- (11)(12)(13) were studied on basal and ACTH stimulated aldosterone production of isolated zona glomerulosa cells and on the corticosterone production of isolated zona fasciculata cells.…”

Section: Introductionmentioning

confidence: 99%

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Structure-activity studies with ACTH/α-MSH fragments on corticosteroid secretion of isolated zona glomerulosa and fasciculata cells

Szalay

Wied

Stark

et al. 1985

Regulatory Peptides

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SummaryThe steroidogenic action of ACTH/~t-MSH fragments was studied on isolated zona glomerulosa and zona fasciculata cells dispersed by collagenase. ACTH-(4-7), ACTH-(6-10), ACTH-(4--10) and ACTH-(11-13) stimulated corticosterone production of the zona fasciculata and aldosterone production of the zona glomerulosa cells. ACTH-(7-10) was ineffective. ACTH-(4-7) appeared to be the most potent peptide of the tested fragments. None of the fragments affected the steroidogenic action of ACTH-(1-39).It is suggested that similar to the melanotropic effect of ~t-MSH two 'message' sequences for adrenocortical stimulation exist in the ~t-MSH part of the ACTH molecule.

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Structure-activity studies with ACTH/α-MSH fragments on corticosteroid secretion of isolated zona glomerulosa and fasciculata cells

Szalay

Wied

Stark

et al. 1985

Regulatory Peptides

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“…with trypsin) on drug response have to be borne in mind using this basis of comparison. Trypsinization occurs at different times with respect to drug exposure in the protocol for intact spheroids and spheroid cells, and trypsinization is known to cause a variety of effects including membrane damage (Hebb & Chu, 1960;Waymouth, 1974). The possibility of trypsin/ cytotoxic drug interaction may be a particular potential problem in the case of adriamycin for which drug the cytotoxicity has been attributed, at least in part, to membrane effects (Tritton & Yee, 1982).…”

mentioning

confidence: 99%

The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids

Kwok¹,

Twentyman²

1985

Br J Cancer

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Summary We have compared the response to a number of cytotoxic drugs of cells treated either within intact multicellular spheroids or as isolated cells following spheroid disaggregation. The cells used were of the EMT6/Ca/VJAC mouse tumour line and spheroids were treated or disaggregated at a mean diameter of 250,um. The response of cell to nitrogen mustard (HN2) or CCNU was similar under the two exposure conditions and we conclude that factors related to spheroid structure (i.e. drug penetrability, intercellular contact effect and microenvironment within the spheroid) do not influence the initial response to these agents. Recovery of potentially lethal damage occuring over 24h, however, greatly modifies the level of cell killing in intact spheroids. EMT6 cells were found to be extremely resistant to vincristine under all exposure conditions. For adriamycin (ADM), cells were always initially more sensitive when exposed to the drug in suspension rather than in intact spheroids. When ADM exposure was prolonged beyond 1 h, however, delaying spheroid disaggregation for 24h led to increased cell kill and reduced differential between the two conditions of exposure. The data suggest that both drug penetration problems and other factors related to spheroid structure are involved in determining the response of cells in small spheroids to ADM.

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“…Enzymatic digestion of tissues can potentially damage the cells during the separation procedure (7). Therefore, the progress in cell 2 separation technology has paralleled improvement in experimental and clinical transplantation results (9).…”

Section: Separation/purificationmentioning

confidence: 99%

Cellular transplantation and gene therapy

Brendel

Ricordi

1994

Transplantation Reviews

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To disaggregate or not to disaggregate, injury and cell disaggregation, transient or permanent?

Cited by 149 publications

References 128 publications

Structure-activity studies with ACTH/α-MSH fragments on corticosteroid secretion of isolated zona glomerulosa and fasciculata cells

Structure-activity studies with ACTH/α-MSH fragments on corticosteroid secretion of isolated zona glomerulosa and fasciculata cells

The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids

Cellular transplantation and gene therapy

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