To exclude or to accumulate? Revealing the role of the sodium HKT1;5 transporter in plant adaptive responses to varying soil salinity

Venkataraman, Gayatri; Shabala, Sergey; Véry, Anne‐Aliénor; Hariharan, G.; Somasundaram, Suji; Pulipati, Shalini; Sellamuthu, Gothandapani; Mohan, Harikrishnan; Kumari, Kumkum; Shabala, Lana; Zhou, Meixue; Chen, Zhong‐Hua

doi:10.1016/j.plaphy.2021.11.030

Cited by 33 publications

(14 citation statements)

References 103 publications

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“…Important ion channels such as potassium and chloride were significantly up-regulated in the tolerant genotypes but repressed in the sensitive genotype. Potassium ion channels exchange Na+ with K+ and regulate the ion-exclusion mechanism [ 62 ]. The potassium transporter gene was induced in the tolerant genotype (Ca29596; FC: 2.37 ↑) while repressed in the sensitive genotype (Ca21839; FC: −5.81 ↓).…”

Section: Resultsmentioning

confidence: 99%

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Kaashyap

Ford

Mann

et al. 2022

Plants

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Salinity is increasingly becoming a significant problem for the most important yet intrinsically salt-sensitive grain legume chickpea. Chickpea is extremely sensitive to salinity during the reproductive phase. Therefore, it is essential to understand the molecular mechanisms by comparing the transcriptomic dynamics between the two contrasting genotypes in response to salt stress. Chickpea exhibits considerable genetic variation amongst improved cultivars, which show better yields in saline conditions but still need to be enhanced for sustainable crop production. Based on previous extensive multi-location physiological screening, two identified genotypes, JG11 (salt-tolerant) and ICCV2 (salt-sensitive), were subjected to salt stress to evaluate their phenological and transcriptional responses. RNA-Sequencing is a revolutionary tool that allows for comprehensive transcriptome profiling to identify genes and alleles associated with stress tolerance and sensitivity. After the first flowering, the whole flower from stress-tolerant and sensitive genotypes was collected. A total of ~300 million RNA-Seq reads were sequenced, resulting in 2022 differentially expressed genes (DEGs) in response to salt stress. Genes involved in flowering time such as FLOWERING LOCUS T (FT) and pollen development such as ABORTED MICROSPORES (AMS), rho-GTPase, and pollen-receptor kinase were significantly differentially regulated, suggesting their role in salt tolerance. In addition to this, we identify a suite of essential genes such as MYB proteins, MADS-box, and chloride ion channel genes, which are crucial regulators of transcriptional responses to salinity tolerance. The gene set enrichment analysis and functional annotation of these genes in flower development suggest that they can be potential candidates for chickpea crop improvement for salt tolerance.

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Section: Resultsmentioning

confidence: 99%

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Kaashyap

Ford

Mann

et al. 2022

Plants

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“…Hence, Na + is compartmentalized into vacuole by Na + /H + antiporter type SODIUM/HYDROGEN EXCHANGER 1 (NHX1) (Fukuda et al, 2004). Similarly, preferential expression of Na + ‐selective influx transporter of the HYDROGEN/POTASSIUM TRANSPORTER 1 (HKT1) family in root xylem parenchyma and in cells adjacent to phloem vessels in leaves leads to the unloading of Na + from the root xylem (Venkataraman et al, 2021). This leads to the retention of this ion in root cells preventing its accumulation in shoot (Chen, Hu, et al, 2015; Chen, Wang, et al, 2015; Ren et al, 2005) OsHKT1 is located at the Saltol QTL identified for salt tolerance (Chen, Hu, et al, 2015; Chen, Wang, et al, 2015).…”

Section: Water Salinity and Heat Stressmentioning

confidence: 99%

Approaches and determinants to sustainably improve crop production

Gojon

Nussaume

Luu

et al. 2022

Food and Energy Security

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Plant scientists and farmers are facing major challenges in providing food and nutritional security for a growing population, while preserving natural resources and biodiversity. Moreover, this should be done while adapting agriculture to climate change and by reducing its carbon footprint. To address these challenges, there is an urgent need to breed crops that are more resilient to suboptimal environments. Huge progress has recently been made in understanding the physiological, genetic and molecular bases of plant nutrition and environmental responses, paving the way towards a more sustainable agriculture. In this review, we present an overview of these progresses and strategies that could be developed to increase plant nutrient use efficiency and tolerance to abiotic stresses. As illustrated by many examples, they already led to promising achievements and crop improvements. Here, we focus on nitrogen and phosphate uptake and use efficiency and on adaptation to drought, salinity and heat stress. These examples first show the necessity of deepening our physiological and molecular understanding of plant environmental responses. In particular, more attention should be paid to investigate stress combinations and stress recovery and acclimation that have been largely neglected to date. It will be necessary to extend these approaches from model plants to crops, to unravel the relevant molecular targets of biotechnological or genetic strategies directly in these species. Similarly, sustained efforts should be done for further exploring the genetic resources available in these species, as well as in wild species adapted to unfavourable environments. Finally, technological developments will be required to breed crops that are more resilient and efficient. This especially relates to the development of multiscale phenotyping under field conditions and a wide range of environments, and use of modelling and big data management to handle the huge amount of information provided by the new molecular, genetic and phenotyping techniques.

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“…Soil salinization is a major global concern for sustainable agriculture production and food security (Ahmed et al, 2021;Venkataraman et al, 2021). Globally, 20% of cultivated and 33% of irrigated agricultural land are affected by salinity, and this proportion is increasing, thus causing severe crop yield losses (Kaashyap et al, 2017(Kaashyap et al, , 2018.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive transcriptomic analysis of two RIL parents with contrasting salt responsiveness identifies polyadenylated and non‐polyadenylated flower lncRNAs in chickpea

Kaashyap

Kaur

Ford

et al. 2022

Plant Biotechnology Journal

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Salinity severely affects the yield of chickpea. Understanding the role of lncRNAs can shed light on chickpea salt tolerance mechanisms. However, because lncRNAs are encoded by multiple sites within the genome, their classification to reveal functional versatility at the transcriptional and the post-transcriptional levels is challenging. To address this, we deep sequenced 24 saltchallenged flower transcriptomes from two parental genotypes of a RIL population that significantly differ in salt tolerance ability. The transcriptomes for the first time included 12 polyadenylated and 12 non-polyadenylated RNA libraries to a sequencing depth of ~50 million reads. The ab initio transcriptome assembly comprised ~34 082 transcripts from three biological replicates of salt-tolerant (JG11) and salt-sensitive (ICCV2) flowers. A total of 9419 lncRNAs responding to salt stress were identified, 2345 of which were novel lncRNAs specific to chickpea. The expression of poly(A+) lncRNAs and naturally antisense transcribed RNAs suggest their role in post-transcriptional modification and gene silencing. Notably, 178 differentially expressed lncRNAs were induced in the tolerant genotype but repressed in the sensitive genotype. Coexpression network analysis revealed that the induced lncRNAs interacted with the FLOWERING LOCUS (FLC), chromatin remodelling and DNA methylation genes, thus inducing flowering during salt stress. Furthermore, 26 lncRNAs showed homology with reported lncRNAs such as COOLAIR, IPS1 and AT4, thus confirming the role of chickpea lncRNAs in controlling flowering time as a crucial salt tolerance mechanism in tolerant chickpea genotype. These robust set of differentially expressed lncRNAs provide a deeper insight into the regulatory mechanisms controlled by lncRNAs under salt stress.

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To exclude or to accumulate? Revealing the role of the sodium HKT1;5 transporter in plant adaptive responses to varying soil salinity

Cited by 33 publications

References 103 publications

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Approaches and determinants to sustainably improve crop production

Comprehensive transcriptomic analysis of two RIL parents with contrasting salt responsiveness identifies polyadenylated and non‐polyadenylated flower lncRNAs in chickpea

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