To Study In-Vitro Antimicrobial Activity of Polyherbal Handwash Formulation

Abstract

The COVID-19 pandemic provides an important reminder that one of the most effective ways to stop the spread of germs and stay healthy is also one of the simplest — handwashing. Numerous side effects and increased antimicrobial drugs resistance encouraged us to discover cost effective, secure formulation of herbal plants. In the present study, research work is focused on evaluating the potential of different formulation incorporated with different herbal extracts against antimicrobe. This Polyherbal formulated handwash presents the efficacious study of 3 herbs consisting Andrographis peniculata, Zingiber officinalis and Citrus limon reflecting antimicrobial activity. The aim of present work is to avoid the adverse effects of synthetic formulation. An attempt has been made to formulate this polyherbal handwash by Maceration method of extraction. Followed by formulating then testing. The antimicrobial activity of prepared handwashes was checked against skin pathogens by diffusion wall method. Different combination of ingredients with plant extracts were tested to evaluate the antimicrobial activity by proper zone of inhibition. Thus, these extracts, showed maximum activity with strains of E. coli i.e., gram negative bacteria and Staphylococcus aureus i.e., gram positive bacteria and were found more potent than marketed preparation. Different evaluation parameter i.e., physical testing, pH, foaming, viscosity etc. were carried out on the formulation of different grades. This study “result” divulges that development of Polyherbal Handwash could have great potential for superficial hygiene of hands in turn thus trigger the good health. The usefulness of polyherbal handwash consisting of Andrographis peniculata, Zingiber officinalis, Citrus limoni against most common pathogens in dermatology, suggesting possible role in treating multidrug-resistant mixed infections. This action may be attributed to the synergistic effects for its use. Thus, proved to be most effective product over Healthy Human Hygiene.

Development and Detection of Antimicrobial Properties of Polyherbal Handwash

“…Aloe vera's pharmacological advantages are ascribed to its capacity to heal wounds as well as its immunomodulatory, anti-inflammatory, antioxidant, and antibacterial capabilities (Figueiredo et al, 2022). Lemon, a member of the Rutaceae family, has cancer-preventive, antibacterial, antifungal, and antidiabetic effects (Rukari et al, 2022). Neem, tulsi, coriander, aloe vera, and lemon juice are all common therapeutic herbs that are employed in the formulation of the polyherbal handwash in this study.…”

“…In this era, the leading infection caused is skin infection which causes non-fatal diseases for a prolonged period of time resulting in expensive treatment (Sharma and Singh, 2020). As per different reports, herbal plants have effective pharmacological activities, especially in dermatological treatments (Rukari et al, 2022). To minimize and combat the bacterial pathogens which affect the skin, the most exposed part of the body, polyherbal handwash was formulated (Aware et al, 2022).…”

“…The extract was filtered once the maceration was completed. The collected filtrate was used for manufacturing of herbal handwash (Bereksi et al, 2018;Kamalapurkar and Shendge, 2022;Rukari et al 2022;Nurcholis et al, 2023).…”