To Study the Relationship Between the Glycosylated Hair Protein and Hba1c for Assessment of Blood Glucose Level in Diabetes Mellitus Type Ii

Abstract

BACKGROUND: Hair is a dead tissue and is composed of keratin and related proteins. Hair is a protein offew growing cells at the base of the hair root. If the patient is diabetic glycosylation occurs in these growingcells.Since the extent of glycation of hair protein varies with the distance from the hair root, we hypothesizedthat glycation of hair protein might provide insight into blood glucose level over a period of several mouthsto one year.So hemoglobin Ale (HbAIc) may be established as an indicator of long-term blood glucosecontrol in diabetic patients. It is formed by non-enzymatic reaction of glucose with Hb.The objective of thisstudy was to estimate the furosine(an intermediate in the breaking down of amadori compounds likeglycosylated proteins) level by HPLC of glycosylated hair proteins, to find interrelation of fasting glucosewith HbAIc and furosine in diabetic patients, to determine the efficacy of Glycosylation of HbA,c andfurosinemay be a reliable marker in diabetic patients.STUDY DESIGN: This study was carried out in the department of Biochemistry Basic Medical SciencesInstitute (BMSI) Jinnah Postgraduate Medical Centre Karachi.METHODS: A total of 200 subjects were included in the study. 100 diagnosed type II and 100 controlnormal subjects. Hair from the scalp 12 cm long and 50 mg in weight was collected from each subject tomeasure furosine level. Furosine level was determined by HPLC and HbAIc, FBSand serumprotein byenzymatic kit method.RESULTS: When the mean values were compared with that of control it depicted significantly high level(0.001) of furosine, HbAIc and FBS.CONCLUSION: The furosine, HbAIc and FBS levels increases in diabetic patients while total serumprotein were unaffected. Weight and BMI increases in diabetic but height was unaffected.KEYWORDS: Diabetes mellitus type II, furosine, HbAIc, fasting blood sugar, glycation ofhair protein