Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection

Emerging Biosensing Approaches for microRNA Analysis

“…Other target regeneration strategies utilized toehold-mediated recycling and nickase based recycling. In both of these approaches, after target recycling the capture probes are introduced to the sample of interest and when the miRNA is present it either linearizes upon hybridization or dissociates from a quenching substrate . The dissociation-based technique eliminated the need for fluorescently-labeled capture probes by staining the hybrid product with an intercalating fluorescent dye.…”

“…888, Huang, R.; Liao, Y.; Zhou, X.; Xing, D. Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection, pp. 162–172 (ref 71). Copyright 2015, with permission from Elsevier.…”

Emerging Approaches for Graphene Oxide Biosensor

“…The system also showed high selectivity of the target against other divalent metal ions. Recently, HCR has been widely adopted for not only simple in vitro analysis but also analytical trials to detect nucleic acids such as mRNA or miRNA within living cells. , …”

“…The system also showed high selectivity of the target against other divalent metal ions. Recently, HCR has been widely adopted for not only simple in vitro analysis 119 but also analytical trials to detect nucleic acids such as mRNA or miRNA within living cells. 120,121 The combination of a GO-based quenching system and HCRbased nonenzymatic signal amplification has also been under investigation for the application of functional analysis on a biomolecule.…”

Cascaded strand displacement for non-enzymatic target recycling amplification and label-free electronic detection of microRNA from tumor cells

“…TSDR can take place without the involvement of any enzymes at room temperature, exhibits high sequence-dependence, and has been widely used in constructing different dynamic DNA devices, including DNA nanomachines [19], DNA circuits [20] and catalytic reaction networks [21]. The application of TSDR for electrochemical detection of inorganic ions [22], proteins [23], DNA and RNA [24,25] has also been reported in recent years.…”